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2016-7-10u2002·u2002HT29-MTX cells were obtained from Dr. Thecla Lesuffleur (INSERM, Paris, France). The cell culture medium for the two types of cells was Dulbecco's Modified Medium containing 1% of nonessential amino acid, 1% of l-glutamine, 1% of penicillin (100 IU/mL) and streptomycin (100 mg/mL) and 10% of fetal bovine serum
Get Price2018-1-1u2002·u20021.1 Workshops on the replacement of FBS. This is the report of the 3rd workshop devoted to the replacement of fetal bovine serum (FBS) in cell and tissue culture methods. The 1st workshop, held in Utrecht, The Netherlands, in 2003 (van der Valk et al., 2004) was initiated after publication of a comprehensive report on pain and distress ...
Get Price2019-10-9u2002·u2002The Matrigel was removed from 3D colonoids and the colonoids were washed with an ice-cold solution of 0.5 mM EDTA in 1× PBS and dissociated with 0.05% trypsin/0.5 mM EDTA at 37°C for 4 min. Following the incubation, the trypsin was inactivated with advanced DMEM/F12, 1× Glutamax, 1× HEPES + 10% fetal bovine serum.
Get Price2019-3-28u2002·u2002drug absorption across the Caco-2 cells (Figure 1). The receiver chamber (185 mL) contained HBSS supplemented with 4% bovine serum albumin (pH 7.4). Digestion studies, in the standard glass reaction vessel or in the upper chamber of the lipolysis-permeation setup, were performed as described previously with minor modifications.18
Get Price2020-10-16u2002·u2002gastrointestinal digestion, were tested as possi - ble modulators of the intestinal permeability of D-chiro-inositol. RESULTS: The absorption of this stereoiso-mer was relatively low and presumably due to passive diffusion, while it was greatly enhanced by the presence of α-Lactalbumin digest. α-Lac-talbumin peptides induced an increase in para-
Get PriceJ.A. Foley. D.E. Otterby. Bovine colostrum imparts passive immunity to newborn calves during the first 24 h of life and generally has been fed for the first 3 days after birth. Surplus colostrum ...
Get Price2012-7-23u2002·u2002Background Gastrointestinal peptides are involved in modulating appetite. Other biological functions attributed to them include the regulation of lipid homeostasis. However, data concerning PYY remain fragmentary. The objectives of the study were: (i) To determine the effect of PYY on intestinal transport and synthesis of cholesterol, the biogenesis of apolipoproteins …
Get Price2021-6-1u2002·u2002The GI digestion products interact with the intestinal lining cells and can be modified and/or pass through them. These processes were simulated using monolayers of Caco2-TC7 cells. The chromatograms corresponding to the assay done with the soluble fraction of APId (1.16 mg peptides/mL) are shown in Fig. 4 .
Get Price2020-6-1u2002·u2002Briefly, Caco-2 cells (HTB-37, ATCC) were cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 4.5 g/L glucose (Sigma, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS ...
Get Price2016-7-10u2002·u2002HT29-MTX cells were obtained from Dr. Thecla Lesuffleur (INSERM, Paris, France). The cell culture medium for the two types of cells was Dulbecco's Modified Medium containing 1% of nonessential amino acid, 1% of l-glutamine, 1% of penicillin (100 IU/mL) and streptomycin (100 mg/mL) and 10% of fetal bovine serum
Get Price2018-1-1u2002·u20021.1 Workshops on the replacement of FBS. This is the report of the 3rd workshop devoted to the replacement of fetal bovine serum (FBS) in cell and tissue culture methods. The 1st workshop, held in Utrecht, The Netherlands, in 2003 (van der Valk et al., 2004) was initiated after publication of a comprehensive report on pain and distress ...
Get Price2019-10-9u2002·u2002The Matrigel was removed from 3D colonoids and the colonoids were washed with an ice-cold solution of 0.5 mM EDTA in 1× PBS and dissociated with 0.05% trypsin/0.5 mM EDTA at 37°C for 4 min. Following the incubation, the trypsin was inactivated with advanced DMEM/F12, 1× Glutamax, 1× HEPES + 10% fetal bovine serum.
Get Price2020-10-9u2002·u2002Caco-2 or MDCK cells (ATCC ® CCL-34™) were seeded at a density of 5×10 3 cells/well in 100 µL of DMEM containing 10% (v/v) fetal bovine serum and 1% penicillin/streptomycin. After cells had been incubated at 37°C for 24 h, they were treated with 100 µL of OP, OP/DLM, and ODSF in free DMEM (equivalent to 1, 10, 25, 50, 100, 250, and 500 ...
Get Price2019-3-28u2002·u2002drug absorption across the Caco-2 cells (Figure 1). The receiver chamber (185 mL) contained HBSS supplemented with 4% bovine serum albumin (pH 7.4). Digestion studies, in the standard glass reaction vessel or in the upper chamber of the lipolysis-permeation setup, were performed as described previously with minor modifications.18
Get Price2020-10-16u2002·u2002gastrointestinal digestion, were tested as possi - ble modulators of the intestinal permeability of D-chiro-inositol. RESULTS: The absorption of this stereoiso-mer was relatively low and presumably due to passive diffusion, while it was greatly enhanced by the presence of α-Lactalbumin digest. α-Lac-talbumin peptides induced an increase in para-
Get PriceJ.A. Foley. D.E. Otterby. Bovine colostrum imparts passive immunity to newborn calves during the first 24 h of life and generally has been fed for the first 3 days after birth. Surplus colostrum ...
Get Price2012-7-23u2002·u2002Background Gastrointestinal peptides are involved in modulating appetite. Other biological functions attributed to them include the regulation of lipid homeostasis. However, data concerning PYY remain fragmentary. The objectives of the study were: (i) To determine the effect of PYY on intestinal transport and synthesis of cholesterol, the biogenesis of apolipoproteins …
Get Price2021-6-1u2002·u2002The GI digestion products interact with the intestinal lining cells and can be modified and/or pass through them. These processes were simulated using monolayers of Caco2-TC7 cells. The chromatograms corresponding to the assay done with the soluble fraction of APId (1.16 mg peptides/mL) are shown in Fig. 4 .
Get Price2020-6-1u2002·u2002Briefly, Caco-2 cells (HTB-37, ATCC) were cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 4.5 g/L glucose (Sigma, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS ...
Get Price2016-7-10u2002·u2002HT29-MTX cells were obtained from Dr. Thecla Lesuffleur (INSERM, Paris, France). The cell culture medium for the two types of cells was Dulbecco's Modified Medium containing 1% of nonessential amino acid, 1% of l-glutamine, 1% of penicillin (100 IU/mL) and streptomycin (100 mg/mL) and 10% of fetal bovine serum
Get Price2018-1-1u2002·u20021.1 Workshops on the replacement of FBS. This is the report of the 3rd workshop devoted to the replacement of fetal bovine serum (FBS) in cell and tissue culture methods. The 1st workshop, held in Utrecht, The Netherlands, in 2003 (van der Valk et al., 2004) was initiated after publication of a comprehensive report on pain and distress ...
Get Price2019-10-9u2002·u2002The Matrigel was removed from 3D colonoids and the colonoids were washed with an ice-cold solution of 0.5 mM EDTA in 1× PBS and dissociated with 0.05% trypsin/0.5 mM EDTA at 37°C for 4 min. Following the incubation, the trypsin was inactivated with advanced DMEM/F12, 1× Glutamax, 1× HEPES + 10% fetal bovine serum.
Get Price2020-10-9u2002·u2002Caco-2 or MDCK cells (ATCC ® CCL-34™) were seeded at a density of 5×10 3 cells/well in 100 µL of DMEM containing 10% (v/v) fetal bovine serum and 1% penicillin/streptomycin. After cells had been incubated at 37°C for 24 h, they were treated with 100 µL of OP, OP/DLM, and ODSF in free DMEM (equivalent to 1, 10, 25, 50, 100, 250, and 500 ...
Get Price2019-3-28u2002·u2002drug absorption across the Caco-2 cells (Figure 1). The receiver chamber (185 mL) contained HBSS supplemented with 4% bovine serum albumin (pH 7.4). Digestion studies, in the standard glass reaction vessel or in the upper chamber of the lipolysis-permeation setup, were performed as described previously with minor modifications.18
Get Price2020-10-16u2002·u2002gastrointestinal digestion, were tested as possi - ble modulators of the intestinal permeability of D-chiro-inositol. RESULTS: The absorption of this stereoiso-mer was relatively low and presumably due to passive diffusion, while it was greatly enhanced by the presence of α-Lactalbumin digest. α-Lac-talbumin peptides induced an increase in para-
Get PriceJ.A. Foley. D.E. Otterby. Bovine colostrum imparts passive immunity to newborn calves during the first 24 h of life and generally has been fed for the first 3 days after birth. Surplus colostrum ...
Get Price2012-7-23u2002·u2002Background Gastrointestinal peptides are involved in modulating appetite. Other biological functions attributed to them include the regulation of lipid homeostasis. However, data concerning PYY remain fragmentary. The objectives of the study were: (i) To determine the effect of PYY on intestinal transport and synthesis of cholesterol, the biogenesis of apolipoproteins (apos) and assembly of ...
Get Price2021-6-1u2002·u2002The GI digestion products interact with the intestinal lining cells and can be modified and/or pass through them. These processes were simulated using monolayers of Caco2-TC7 cells. The chromatograms corresponding to the assay done with the soluble fraction of APId (1.16 mg peptides/mL) are shown in Fig. 4 .
Get Price2020-6-1u2002·u2002Briefly, Caco-2 cells (HTB-37, ATCC) were cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 4.5 g/L glucose (Sigma, St. Louis, MO, …
Get Price2016-7-10u2002·u2002HT29-MTX cells were obtained from Dr. Thecla Lesuffleur (INSERM, Paris, France). The cell culture medium for the two types of cells was Dulbecco's Modified Medium containing 1% of nonessential amino acid, 1% of l-glutamine, 1% of penicillin (100 IU/mL) and streptomycin (100 mg/mL) and 10% of fetal bovine serum
Get Price2018-1-1u2002·u20021.1 Workshops on the replacement of FBS. This is the report of the 3rd workshop devoted to the replacement of fetal bovine serum (FBS) in cell and tissue culture methods. The 1st workshop, held in Utrecht, The Netherlands, in 2003 (van der Valk et al., 2004) was initiated after publication of a comprehensive report on pain and distress ...
Get Price2019-10-9u2002·u2002The Matrigel was removed from 3D colonoids and the colonoids were washed with an ice-cold solution of 0.5 mM EDTA in 1× PBS and dissociated with 0.05% trypsin/0.5 mM EDTA at 37°C for 4 min. Following the incubation, the trypsin was inactivated with advanced DMEM/F12, 1× Glutamax, 1× HEPES + 10% fetal bovine serum.
Get Price2020-10-9u2002·u2002Caco-2 or MDCK cells (ATCC ® CCL-34™) were seeded at a density of 5×10 3 cells/well in 100 µL of DMEM containing 10% (v/v) fetal bovine serum and 1% penicillin/streptomycin. After cells had been incubated at 37°C for 24 h, they were treated with 100 µL of OP, OP/DLM, and ODSF in free DMEM (equivalent to 1, 10, 25, 50, 100, 250, and 500 ...
Get Price2019-3-28u2002·u2002drug absorption across the Caco-2 cells (Figure 1). The receiver chamber (185 mL) contained HBSS supplemented with 4% bovine serum albumin (pH 7.4). Digestion studies, in the standard glass reaction vessel or in the upper chamber of the lipolysis-permeation setup, were performed as described previously with minor modifications.18
Get Price2020-10-16u2002·u2002gastrointestinal digestion, were tested as possi - ble modulators of the intestinal permeability of D-chiro-inositol. RESULTS: The absorption of this stereoiso-mer was relatively low and presumably due to passive diffusion, while it was greatly enhanced by the presence of α-Lactalbumin digest. α-Lac-talbumin peptides induced an increase in para-
Get PriceJ.A. Foley. D.E. Otterby. Bovine colostrum imparts passive immunity to newborn calves during the first 24 h of life and generally has been fed for the first 3 days after birth. Surplus colostrum ...
Get Price2012-7-23u2002·u2002Background Gastrointestinal peptides are involved in modulating appetite. Other biological functions attributed to them include the regulation of lipid homeostasis. However, data concerning PYY remain fragmentary. The objectives of the study were: (i) To determine the effect of PYY on intestinal transport and synthesis of cholesterol, the biogenesis of apolipoproteins (apos) and assembly of ...
Get Price2021-6-1u2002·u2002The GI digestion products interact with the intestinal lining cells and can be modified and/or pass through them. These processes were simulated using monolayers of Caco2-TC7 cells. The chromatograms corresponding to the assay done with the soluble fraction of APId (1.16 mg peptides/mL) are shown in Fig. 4 .
Get Price2020-6-1u2002·u2002Briefly, Caco-2 cells (HTB-37, ATCC) were cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 4.5 g/L glucose (Sigma, St. Louis, MO, …
Get Price2016-7-10u2002·u2002HT29-MTX cells were obtained from Dr. Thecla Lesuffleur (INSERM, Paris, France). The cell culture medium for the two types of cells was Dulbecco's Modified Medium containing 1% of nonessential amino acid, 1% of l-glutamine, 1% of penicillin (100 IU/mL) and streptomycin (100 mg/mL) and 10% of fetal bovine serum
Get Price2018-1-1u2002·u20021.1 Workshops on the replacement of FBS. This is the report of the 3rd workshop devoted to the replacement of fetal bovine serum (FBS) in cell and tissue culture methods. The 1st workshop, held in Utrecht, The Netherlands, in 2003 (van der Valk et al., 2004) was initiated after publication of a comprehensive report on pain and distress ...
Get Price2019-10-9u2002·u2002The Matrigel was removed from 3D colonoids and the colonoids were washed with an ice-cold solution of 0.5 mM EDTA in 1× PBS and dissociated with 0.05% trypsin/0.5 mM EDTA at 37°C for 4 min. Following the incubation, the trypsin was inactivated with advanced DMEM/F12, 1× Glutamax, 1× HEPES + 10% fetal bovine serum.
Get Price2020-10-9u2002·u2002Caco-2 or MDCK cells (ATCC ® CCL-34™) were seeded at a density of 5×10 3 cells/well in 100 µL of DMEM containing 10% (v/v) fetal bovine serum and 1% penicillin/streptomycin. After cells had been incubated at 37°C for 24 h, they were treated with 100 µL of OP, OP/DLM, and ODSF in free DMEM (equivalent to 1, 10, 25, 50, 100, 250, and 500 ...
Get Price2019-3-28u2002·u2002drug absorption across the Caco-2 cells (Figure 1). The receiver chamber (185 mL) contained HBSS supplemented with 4% bovine serum albumin (pH 7.4). Digestion studies, in the standard glass reaction vessel or in the upper chamber of the lipolysis-permeation setup, were performed as described previously with minor modifications.18
Get Price2020-10-16u2002·u2002gastrointestinal digestion, were tested as possi - ble modulators of the intestinal permeability of D-chiro-inositol. RESULTS: The absorption of this stereoiso-mer was relatively low and presumably due to passive diffusion, while it was greatly enhanced by the presence of α-Lactalbumin digest. α-Lac-talbumin peptides induced an increase in para-
Get PriceJ.A. Foley. D.E. Otterby. Bovine colostrum imparts passive immunity to newborn calves during the first 24 h of life and generally has been fed for the first 3 days after birth. Surplus colostrum ...
Get Price2012-7-23u2002·u2002Background Gastrointestinal peptides are involved in modulating appetite. Other biological functions attributed to them include the regulation of lipid homeostasis. However, data concerning PYY remain fragmentary. The objectives of the study were: (i) To determine the effect of PYY on intestinal transport and synthesis of cholesterol, the biogenesis of apolipoproteins (apos) and assembly of ...
Get Price2021-6-1u2002·u2002The GI digestion products interact with the intestinal lining cells and can be modified and/or pass through them. These processes were simulated using monolayers of Caco2-TC7 cells. The chromatograms corresponding to the assay done with the soluble fraction of APId (1.16 mg peptides/mL) are shown in Fig. 4 .
Get Price2020-6-1u2002·u2002Briefly, Caco-2 cells (HTB-37, ATCC) were cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 4.5 g/L glucose (Sigma, St. Louis, MO, …
Get Price2016-7-10u2002·u2002HT29-MTX cells were obtained from Dr. Thecla Lesuffleur (INSERM, Paris, France). The cell culture medium for the two types of cells was Dulbecco's Modified Medium containing 1% of nonessential amino acid, 1% of l-glutamine, 1% of penicillin (100 IU/mL) and streptomycin (100 mg/mL) and 10% of fetal bovine serum
Get Price2018-1-1u2002·u20021.1 Workshops on the replacement of FBS. This is the report of the 3rd workshop devoted to the replacement of fetal bovine serum (FBS) in cell and tissue culture methods. The 1st workshop, held in Utrecht, The Netherlands, in 2003 (van der Valk et al., 2004) was initiated after publication of a comprehensive report on pain and distress ...
Get Price2019-10-9u2002·u2002The Matrigel was removed from 3D colonoids and the colonoids were washed with an ice-cold solution of 0.5 mM EDTA in 1× PBS and dissociated with 0.05% trypsin/0.5 mM EDTA at 37°C for 4 min. Following the incubation, the trypsin was inactivated with advanced DMEM/F12, 1× Glutamax, 1× HEPES + 10% fetal bovine serum.
Get Price2020-10-9u2002·u2002Caco-2 or MDCK cells (ATCC ® CCL-34™) were seeded at a density of 5×10 3 cells/well in 100 µL of DMEM containing 10% (v/v) fetal bovine serum and 1% penicillin/streptomycin. After cells had been incubated at 37°C for 24 h, they were treated with 100 µL of OP, OP/DLM, and ODSF in free DMEM (equivalent to 1, 10, 25, 50, 100, 250, and 500 ...
Get Price2019-3-28u2002·u2002drug absorption across the Caco-2 cells (Figure 1). The receiver chamber (185 mL) contained HBSS supplemented with 4% bovine serum albumin (pH 7.4). Digestion studies, in the standard glass reaction vessel or in the upper chamber of the lipolysis-permeation setup, were performed as described previously with minor modifications.18
Get Price2020-10-16u2002·u2002gastrointestinal digestion, were tested as possi - ble modulators of the intestinal permeability of D-chiro-inositol. RESULTS: The absorption of this stereoiso-mer was relatively low and presumably due to passive diffusion, while it was greatly enhanced by the presence of α-Lactalbumin digest. α-Lac-talbumin peptides induced an increase in para-
Get PriceJ.A. Foley. D.E. Otterby. Bovine colostrum imparts passive immunity to newborn calves during the first 24 h of life and generally has been fed for the first 3 days after birth. Surplus colostrum ...
Get Price2012-7-23u2002·u2002Background Gastrointestinal peptides are involved in modulating appetite. Other biological functions attributed to them include the regulation of lipid homeostasis. However, data concerning PYY remain fragmentary. The objectives of the study were: (i) To determine the effect of PYY on intestinal transport and synthesis of cholesterol, the biogenesis of apolipoproteins (apos) and assembly of ...
Get Price2021-6-1u2002·u2002The GI digestion products interact with the intestinal lining cells and can be modified and/or pass through them. These processes were simulated using monolayers of Caco2-TC7 cells. The chromatograms corresponding to the assay done with the soluble fraction of APId (1.16 mg peptides/mL) are shown in Fig. 4 .
Get Price2020-6-1u2002·u2002Briefly, Caco-2 cells (HTB-37, ATCC) were cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 4.5 g/L glucose (Sigma, St. Louis, MO, USA) supplemented with 10% fetal bovine serum …
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