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  • The Influence of Micronutrients in Cell Culture: A ...

    2013-5-27u2002·u2002In many common culture media, the sole source of micronutrients is fetal bovine serum (FBS), which contributes to only 5–10% of the media composition. Minimal attention has been dedicated to FBS composition, micronutrients in cell cultures as a whole, or the influence of micronutrients on the viability and genetics of cultured cells.

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  • Flexible gastrointestinal endoscope processing challenges ...

    2021-4-1u2002·u2002The results for each endoscope model (Pentax EC38-i10L, Olympus HQ190L and Olympus TJF Q180V) showed that all channels in top and bottom trays became sterile in the presence of a soil load containing G. stearothermophilus spores (>10 6 cfu) …

    Get Price
  • Improved in vitro bioavailability of a newly developed ...

    2021-6-30u2002·u20021. Introduction. Calcium (Ca) is an essential nutrient necessary for many functions in human health such as nerve conduction, muscle and vascular contraction, mitosis, blood coagulation, hormonal secretion and structural support of the skeleton (Miller, Jarvis & McBean, 2001).It is the most abundant mineral in the body with 99% found in teeth and bone and only 1% is found in serum.

    Get Price
  • A LCMS Metabolomic Workflow to Investigate Metabolic ...

    Human intestinal Caco-2 cells were purchased from the American Type Culture Collection (ATCC Cat# HTB-37, RRID:CVCL 0025) at passage 17. The cells were continuously cultured (37°C, 5% CO 2, 95% humidified air) in Minimum Essential Medium (MEM) supplemented with 10% Fetal Bovine Serum (FBS) and Normocin (InvivoGen, San Diego, United States) and ...

    Get Price
  • Oral administration of interferon-α2b-transformed ...

    2011-12-8u2002·u2002Introduction. The oral use of low doses of interferon (IFN)-α has been shown to exhibit beneficial effects in mice or human with acquired immunodeficiency syndrome (AIDS) [], hepatitis B [], aphthous stomatitis [], and measles [].These studies have indicated that IFN-α/-β given orally provides a viable alternative to the current high-dose treatment intramuscularly [5,6].

    Get Price
  • In vitro analysis of gastrointestinal tolerance and ...

    2012-12-1u2002·u2002Effect of carrier food type on in vitro gastrointestinal survival and adhesion ability of probiotic Lactobacillus acidophilus LA-5, Bifidobacterium animalis subsp. lactis BB-12 and Propionibacterium jensenii 702 were evaluated using goat's milk ice cream, plain and fruit yogurts. Carrier food matrix had a significant influence on the in vitro gastrointestinal tolerance of all three probiotics ...

    Get Price
  • Modulatory Role of PYY in Transport and Metabolism of ...

    2012-7-23u2002·u2002Background Gastrointestinal peptides are involved in modulating appetite. Other biological functions attributed to them include the regulation of lipid homeostasis. However, data concerning PYY remain fragmentary. The objectives of the study were: (i) To determine the effect of PYY on intestinal transport and synthesis of cholesterol, the biogenesis of apolipoproteins (apos) and assembly of ...

    Get Price
  • Intracellular antioxidant activity and intestinal ...

    2021-6-1u2002·u2002The GI digestion products interact with the intestinal lining cells and can be modified and/or pass through them. These processes were simulated using monolayers of Caco2-TC7 cells. The chromatograms corresponding to the assay done with the soluble fraction of APId (1.16 mg peptides/mL) are shown in Fig. 4 .

    Get Price
  • Enhanced stability of berry pomace polyphenols delivered ...

    2020-11-30u2002·u2002Stability of protein-polyphenol aggregate particles, created by complexing polyphenols from blueberry and muscadine grape pomaces with a rice-pea protein isolate blend, was evaluated in an in vitro gastrointestinal model. Recovery index (RI; % total phenolics present post-digestion) was 69% and 62% from blueberry and muscadine grape protein-polyphenol particles, compared to 23% and 31% for …

    Get Price
  • Rosmarinus officinalis L. Essential Oil Protects ...

    2018-12-12u2002·u2002Journal of haracolog Clinical Research How to cite this article: Abashwashi M, Palomino O, Gómez-Serranillos M. Rosmarinus officinalis L. Essential Oil Protects Astrocytes against Oxidative 002 Damage. J of Pharmacol & Clin Res. 2017; 2(5): 555598. DOI: 10.19080/JPCR.2017.02.555598.

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  • Title: Iron bioavailability from commercially available ...

    2016-3-7u2002·u2002v/v fetal bovine serum (LCG Standards, 30-2020), 1% penicillin-streptomycin, 4 mmol/L L-glutamine, 1% non-essential amino acids, and Plasmocin 5 mg/ml (Source Bioscience). 24 hours prior to the initiation of in vitro digestion experiments media was changed to supplemented MEM without foetal bovine serum but with 10 mmol/L PIPES

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  • Pharmacological Modulation of Immune Responses by ...

    2021-10-1u2002·u2002The incidence of noncommunicable diseases (NCDs) has increased over the last few decades, and one of the major contributors to this is lifestyle, especially diet. High intake of saturated fatty acids and low intake of dietary fiber is linked to an increase in NCDs. Conversely, a low intake of saturated fatty acids and a high intake of dietary fiber seem to have a protective effect on general ...

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  • The potential probiotic Lactobacillus rhamnosus CNCM I ...

    2019-4-1u2002·u2002The genus Lactobacillus is a phylogenetically diverse group of Gram-positive bacteria. It includes more than 200 species found in diverse ecosystems, including the …

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  • Inhibition of the facilitative sugar transporters (GLUTs ...

    2021-6-25u2002·u2002erties by inhibiting sugar transporters in the small intestine and improving insulin sensitivity. In this report, we studied the effects of teas and tea catechins on the small intestinal sugar transporters, SGLT1 and GLUTs (GLUT1, 2 and 5). Green tea extract (GT), oolong tea extract (OT), and black tea extract (BT) inhibited glu-

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  • Sci-Hub

    Sci-Hub,mg.scihub.ltd,sci-hub.tw,The project is supported by user donations. Imagine the world with free access to knowledge for everyone ‐ a world without any paywalls.

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  • Fermentation products of Danshen relieved dextran sulfate ...

    2021-8-10u2002·u2002Cell supernatants, serum and tissue homogenates were collected respectively for the determination of IL-6, IL-1β and TNF-α concentrations according to the manufacturers' instructions ...

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  • Bromelain and N-acetylcysteine inhibit proliferation and ...

    2014-11-12u2002·u2002Bromelain and N-acetylcysteine are two natural, sulfhydryl-containing compounds with good safety profiles which have been investigated for their benefits and application in health and disease for more than fifty years. As such, the potential values of these agents in cancer therapy have been variably reported in the literature. In the present study, the efficacy of bromelain and N ...

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  • Effects of Boswellia serrata in mouse models of chemically ...

    2005-4-1u2002·u2002Extracts from Boswellia serrata have been reported to have anti-inflammatory activity, primarily via boswellic acid-mediated inhibition of leukotriene synthesis. In three small clinical trials, boswellia was shown to improve symptoms of ulcerative colitis and Crohn's disease, and because of its alleged safety, boswellia was considered superior over mesalazine in terms of a benefit-risk ...

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  • Fothergill, Linda J., Callaghan, Brid, Rivera, Leni R ...

    nutrients Article Effects of Food Components That Activate TRPA1 Receptors on Mucosal Ion Transport in the Mouse Intestine Linda J. Fothergill 1,*, Brid Callaghan 1, Leni R. Rivera 1,2, TinaMarie Lieu 3, Daniel P. Poole 1,3, Hyun-Jung Cho 4, David M. Bravo 5 and John B. Furness 1,6,* 1 Department of Anatomy & Neuroscience, University of Melbourne, Parkville VIC 3010, Australia;

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  • Oral Ursodeoxycholic Acid Crosses the Blood Retinal ...

    2021-2-3u2002·u2002The main function of BA is the emulsification, absorption, and digestion of lipids. However, the hydrophilic secondary BAs, ursodeoxycholic acid (UDCA) and tauroursodeoxycholic acid (TUDCA), the taurine conjugate of UDCA, have also shown neuroprotective effects in neurodegenerative [ 5 ] and retinal disease [ 6 ].

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  • The Influence of Micronutrients in Cell Culture: A ...

    2013-5-27u2002·u2002In many common culture media, the sole source of micronutrients is fetal bovine serum (FBS), which contributes to only 5–10% of the media composition. Minimal attention has been dedicated to FBS composition, micronutrients in cell cultures as a whole, or the influence of micronutrients on the viability and genetics of cultured cells.

    Get Price
  • Flexible gastrointestinal endoscope processing challenges ...

    2021-4-1u2002·u2002The results for each endoscope model (Pentax EC38-i10L, Olympus HQ190L and Olympus TJF Q180V) showed that all channels in top and bottom trays became sterile in the presence of a soil load containing G. stearothermophilus spores (>10 6 cfu) in 300 ppm AOAC hard water and 5% fetal bovine serum.

    Get Price
  • Improved in vitro bioavailability of a newly developed ...

    2021-6-30u2002·u20021. Introduction. Calcium (Ca) is an essential nutrient necessary for many functions in human health such as nerve conduction, muscle and vascular contraction, mitosis, blood coagulation, hormonal secretion and structural support of the skeleton (Miller, Jarvis & McBean, 2001).It is the most abundant mineral in the body with 99% found in teeth and bone and only 1% is found in serum.

    Get Price
  • A LCMS Metabolomic Workflow to Investigate Metabolic ...

    Human intestinal Caco-2 cells were purchased from the American Type Culture Collection (ATCC Cat# HTB-37, RRID:CVCL 0025) at passage 17. The cells were continuously cultured (37°C, 5% CO 2, 95% humidified air) in Minimum Essential Medium (MEM) supplemented with 10% Fetal Bovine Serum (FBS) and Normocin (InvivoGen, San Diego, United States) and ...

    Get Price
  • Oral administration of interferon-α2b-transformed ...

    2011-12-8u2002·u2002Introduction. The oral use of low doses of interferon (IFN)-α has been shown to exhibit beneficial effects in mice or human with acquired immunodeficiency syndrome (AIDS) [], hepatitis B [], aphthous stomatitis [], and measles [].These studies have indicated that IFN-α/-β given orally provides a viable alternative to the current high-dose treatment intramuscularly [5,6].

    Get Price
  • In vitro analysis of gastrointestinal tolerance and ...

    2012-12-1u2002·u2002Effect of carrier food type on in vitro gastrointestinal survival and adhesion ability of probiotic Lactobacillus acidophilus LA-5, Bifidobacterium animalis subsp. lactis BB-12 and Propionibacterium jensenii 702 were evaluated using goat's milk ice cream, plain and fruit yogurts. Carrier food matrix had a significant influence on the in vitro gastrointestinal tolerance of all three probiotics ...

    Get Price
  • Modulatory Role of PYY in Transport and Metabolism of ...

    2012-7-23u2002·u2002Background Gastrointestinal peptides are involved in modulating appetite. Other biological functions attributed to them include the regulation of lipid homeostasis. However, data concerning PYY remain fragmentary. The objectives of the study were: (i) To determine the effect of PYY on intestinal transport and synthesis of cholesterol, the biogenesis of apolipoproteins (apos) and assembly of ...

    Get Price
  • Intracellular antioxidant activity and intestinal ...

    2021-6-1u2002·u2002The GI digestion products interact with the intestinal lining cells and can be modified and/or pass through them. These processes were simulated using monolayers of Caco2-TC7 cells. The chromatograms corresponding to the assay done with the soluble fraction of APId (1.16 mg peptides/mL) are shown in Fig. 4 .

    Get Price
  • Enhanced stability of berry pomace polyphenols delivered ...

    2020-11-30u2002·u2002Stability of protein-polyphenol aggregate particles, created by complexing polyphenols from blueberry and muscadine grape pomaces with a rice-pea protein isolate blend, was evaluated in an in vitro gastrointestinal model. Recovery index (RI; % total phenolics present post-digestion) was 69% and 62% from blueberry and muscadine grape protein-polyphenol particles, compared to 23% and 31% for …

    Get Price
  • Rosmarinus officinalis L. Essential Oil Protects ...

    2018-12-12u2002·u2002Journal of haracolog Clinical Research How to cite this article: Abashwashi M, Palomino O, Gómez-Serranillos M. Rosmarinus officinalis L. Essential Oil Protects Astrocytes against Oxidative 002 Damage. J of Pharmacol & Clin Res. 2017; 2(5): 555598. DOI: 10.19080/JPCR.2017.02.555598.

    Get Price
  • Oral bovine lactoferrin improves bone status of ...

    2009-6-1u2002·u2002The aim of the present study was to evaluate the effect of dietary lactoferrin on bone metabolism in vivo using a postmenopausal animal model. We investigated whether bovine lactoferrin (bLF) ingestion could prevent bone loss in ovariectomized mice. Twelve-week-old female C3H mice either ovariectomized or sham operated were fed for 27 wk with the control diet (AIN …

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  • Oral administration of interferon-α2b-transformed ...

    2011-12-8u2002·u2002Multiple reports have claimed that low-dose orally administered interferon (IFN)-α is beneficial in the treatment of many infectious diseases and provides a viable alternative to high-dose intramuscular treatment. However, research is needed on how to express IFN stably in the gut. Bifidobacterium may be a suitable carrier for human gene expression and secretion in the …

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  • Sci-Hub: removing barriers in the way of science

    The Sci-Hub project supports Open Access movement in science. Research should be published in open access, i.e. be free to read. The Open Access is a new and advanced form of scientific communication, which is going to replace outdated subscription models.

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  • Sucrosomial® Iron Supplementation in Mice: Effects on ...

    Eagle's Medium respectively, containing 10% endotoxin-free fetal bovine serum (FBS) (Sigma-Aldrich), 0.04 mg/mL gentamicin (Gibco), and 2 mM L -glutamine (Gibco) at 37 C under an atmosphere of 5 ...

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  • Role of Leptin in the Regulation of Glucagon-Like Peptide ...

    Glucagon-like peptide-1 (GLP-1), released from intestinal endocrine L cells, is a potent insulinotropic hormone. GLP-1 secretion is diminished in obese patients. Because obesity is linked to abnormal leptin signaling, we hypothesized that leptin may modulate GLP-1 secretion. Leptin significantly stimulated GLP-1 secretion (by up to 250% of control) from fetal rat intestinal cells, …

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  • R.L. Baldwin's research works

    R.L. Baldwin's 139 research works with 5,298 citations and 13,692 reads, including: Evaluating model predictions of partitioning nitrogen excretion using the dairy cow model, Molly

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  • 'Oil-soluble' reversed lipid nanoparticles for oral ...

    2020-7-17u2002·u2002In this study, we aimed to design a novel oral insulin delivery system, named 'oil-soluble' reversed lipid nanoparticles (ORLN), in which a hydrophilic insulin molecule is encapsulated by a phospholipid (PC) shell and dissolved in oil to prevent the enzymatic degradation of insulin. ORLN was characterized by transmission electron microscopy and dynamic light scattering.

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  • Organization of lipids in milks, infant milk formulas and ...

    The microstructure of milk fat in processed dairy products is poorly known despite its importance in their functional, sensorial and nutritional properties. However, for the last 10 years, several research groups including our laboratory have significantly contributed to increasing knowledge on the organization of lipids in situ in dairy products. This paper provides an overview of recent ...

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  • Food proteins as a source of bioactive peptides with ...

    2012-8-1u2002·u2002Intact bioactive proteins and peptides with direct effects on the intestine. Milk is a fluid specifically designed to meet the nutritional and developmental needs of the new-born and undoubtedly has a role beyond nutrition, acting in the infant luminally in the gut at the mucosal surface and after absorption acting systemically to affect gut maturation (Reference Kelly and …

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  • Intraperitoneally infused human mesenchymal stem cells ...

    2016-2-10u2002·u2002Mesenchymal stem/progenitor cells (MSC) have shown beneficial effects in many models of disease in part by modulating excessive inflammatory and immune responses. Frequently the beneficial effects of MSC persist long after their disappearance from host tissues, suggesting that MSC interact with intermediate cells in the host that relay or amplify their effects.

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  • The Influence of Micronutrients in Cell Culture: A ...

    2013-5-27u2002·u2002In many common culture media, the sole source of micronutrients is fetal bovine serum (FBS), which contributes to only 5–10% of the media composition. Minimal attention has been dedicated to FBS composition, micronutrients in cell cultures as a whole, or the influence of micronutrients on the viability and genetics of cultured cells.

    Get Price
  • Flexible gastrointestinal endoscope processing challenges ...

    2021-4-1u2002·u2002The results for each endoscope model (Pentax EC38-i10L, Olympus HQ190L and Olympus TJF Q180V) showed that all channels in top and bottom trays became sterile in the presence of a soil load containing G. stearothermophilus spores (>10 6 cfu) …

    Get Price
  • Improved in vitro bioavailability of a newly developed ...

    2021-6-30u2002·u20021. Introduction. Calcium (Ca) is an essential nutrient necessary for many functions in human health such as nerve conduction, muscle and vascular contraction, mitosis, blood coagulation, hormonal secretion and structural support of the skeleton (Miller, Jarvis & McBean, 2001).It is the most abundant mineral in the body with 99% found in teeth and bone and only 1% is found in serum.

    Get Price
  • A LCMS Metabolomic Workflow to Investigate Metabolic ...

    Human intestinal Caco-2 cells were purchased from the American Type Culture Collection (ATCC Cat# HTB-37, RRID:CVCL 0025) at passage 17. The cells were continuously cultured (37°C, 5% CO 2, 95% humidified air) in Minimum Essential Medium (MEM) supplemented with 10% Fetal Bovine Serum (FBS) and Normocin (InvivoGen, San Diego, United States) and ...

    Get Price
  • Oral administration of interferon-α2b-transformed ...

    2011-12-8u2002·u2002Introduction. The oral use of low doses of interferon (IFN)-α has been shown to exhibit beneficial effects in mice or human with acquired immunodeficiency syndrome (AIDS) [], hepatitis B [], aphthous stomatitis [], and measles [].These studies have indicated that IFN-α/-β given orally provides a viable alternative to the current high-dose treatment intramuscularly [5,6].

    Get Price
  • In vitro analysis of gastrointestinal tolerance and ...

    2012-12-1u2002·u2002Effect of carrier food type on in vitro gastrointestinal survival and adhesion ability of probiotic Lactobacillus acidophilus LA-5, Bifidobacterium animalis subsp. lactis BB-12 and Propionibacterium jensenii 702 were evaluated using goat's milk ice cream, plain and fruit yogurts. Carrier food matrix had a significant influence on the in vitro gastrointestinal tolerance of all three probiotics ...

    Get Price
  • Modulatory Role of PYY in Transport and Metabolism of ...

    2012-7-23u2002·u2002Background Gastrointestinal peptides are involved in modulating appetite. Other biological functions attributed to them include the regulation of lipid homeostasis. However, data concerning PYY remain fragmentary. The objectives of the study were: (i) To determine the effect of PYY on intestinal transport and synthesis of cholesterol, the biogenesis of apolipoproteins (apos) and assembly of ...

    Get Price
  • Intracellular antioxidant activity and intestinal ...

    2021-6-1u2002·u2002The GI digestion products interact with the intestinal lining cells and can be modified and/or pass through them. These processes were simulated using monolayers of Caco2-TC7 cells. The chromatograms corresponding to the assay done with the soluble fraction of APId (1.16 mg peptides/mL) are shown in Fig. 4 .

    Get Price
  • Enhanced stability of berry pomace polyphenols delivered ...

    2020-11-30u2002·u2002Stability of protein-polyphenol aggregate particles, created by complexing polyphenols from blueberry and muscadine grape pomaces with a rice-pea protein isolate blend, was evaluated in an in vitro gastrointestinal model. Recovery index (RI; % total phenolics present post-digestion) was 69% and 62% from blueberry and muscadine grape protein-polyphenol particles, compared to 23% and 31% for …

    Get Price
  • Rosmarinus officinalis L. Essential Oil Protects ...

    2018-12-12u2002·u2002Journal of haracolog Clinical Research How to cite this article: Abashwashi M, Palomino O, Gómez-Serranillos M. Rosmarinus officinalis L. Essential Oil Protects Astrocytes against Oxidative 002 Damage. J of Pharmacol & Clin Res. 2017; 2(5): 555598. DOI: 10.19080/JPCR.2017.02.555598.

    Get Price
  • Role of Gastrointestinal Hormones in the Proliferation of ...

    Gastrointestinal (GI) hormones are chemical messengers that regulate the physiological functions of the intestine and pancreas, including secretion, motility, absorption, and digestion. In addition to these well-defined physiological effects, GI hormones can stimulate proliferation of the nonneoplastic intestinal mucosa and pancreas.

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  • Early-Childhood Membranous Nephropathy Due to

    2019-9-10u2002·u2002Although direct interaction of cationic bovine serum albumin with the anionic glomerular capillary wall most likely is the triggering event, one can speculate that circulating IgG4-containing ...

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  • The quantitative assessment of body iron

    2003-5-1u2002·u2002The serum ferritin assay was standardized with recrystallized human liver ferritin that was diluted to 1000 μg/L in buffered bovine serum albumin (BSA) and stored in aliquots at −70°C. Fresh standard material was thawed at the beginning of each investigation and was calibrated against WHO International Standard 80-578, obtained from the ...

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  • Development of an enhanced human gastrointestinal ...

    2013-9-21u2002·u2002Objective The technology for the growth of human intestinal epithelial cells is rapidly progressing. An exciting possibility is that this system could serve as a platform for individualised medicine and research. However, to achieve this goal, human epithelial culture must be enhanced so that biopsies from individuals can be used to reproducibly generate cell lines in a short time frame so ...

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  • Induced migration of endothelial cells into 3D scaffolds ...

    2017-4-11u2002·u2002The medium was then extracted, and the released cells were collected by centrifugation at 1000 rpm/min. Next, 3 × 10 5 cells were incubated in a well of 24 well culture plate containing 1 ml DMEM supplemented with 100 U/ml penicillin and 100 µg/ml streptomycin, 10% fetal bovine serum and 0.05 mM 2-mercaptoethanol at 37ºC and 5% CO 2.

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  • Home - DeLaval

    Emerging Stronger Together. Maintaining a 135-year legacy of innovations for dairy farmers doesn't come without its challenges – including pandemics and crises. Throughout two world wars and several pandemics and outbreaks, DeLaval has adapted to continue providing world-class solutions to dairy farmers around the globe.

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  • Bioactive proteins in breast milk - Lönnerdal - 2013 ...

    2013-3-1u2002·u2002Human milk proteins may be largely resistant against digestion in the gastrointestinal tract, be partially digested into bioactive peptides, or be more or less completely digested and utilised as a source of amino acids. These events can be studied using an in vitro digestion model, which is useful for predicting results in human infants. Some ...

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  • The use of biochar in animal feeding [PeerJ]

    2019-5-7u2002·u2002Biochar, that is, carbonized biomass similar to charcoal, has been used in acute medical treatment of animals for many centuries. Since 2010, livestock farmers increasingly use biochar as a regular feed supplement to improve animal health, increase nutrient intake efficiency and thus productivity. As biochar gets enriched with nitrogen-rich organic compounds during the digestion …

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  • Two million children innoculated with BSE vaccines

    2001-2-12u2002·u20024. J have a measles vaccine using bovine serum from the UK. There are 440,000 units of stock. They have also got MMR using bovine serum from the UK. 5. K have influenza, rubella, measles, MMR vaccines likely to be used in children. Of those they think that only MMR contains bovine material which is probably a French origin. 6.

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  • Antihypertensive Peptides from Food Proteins

    2013-1-30u2002·u2002Antihypertensive peptides from milk, egg, animal (including meat and marine animals), plant and macroalgae have recently been reviewed [4; 25; 83-86]. Ile-Pro-Pro (β-casein f74-76; κ-casein f108-110) and Val-Pro-Pro (β-casein f84-86) were among the first dietary peptides found to …

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