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  • Intestinal NHE8 is highly expressed in goblet cells and ...

    2015-10-21u2002·u2002the intestine plays an important role in digestion and absorption, the mucus lining the epithelium represents a pivotal function in mucosal protection. Goblet cells are scattered in both the crypts and among enterocytes, and they secrete an important component of mucus, mucin. We have reported that sodium/hydrogen exchanger (NHE) 8 is

    Get Price
  • Enhancement of D-chiro-inositol transport across ...

    2020-10-16u2002·u2002gastrointestinal digestion, were tested as possi - ble modulators of the intestinal permeability of D-chiro-inositol. RESULTS: The absorption of this stereoiso-mer was relatively low and presumably due to passive diffusion, while it was greatly enhanced by the presence of α-Lactalbumin digest. α-Lac-talbumin peptides induced an increase in para-

    Get Price
  • Lipolysis-Permeation Setup for Simultaneous Study of ...

    2019-3-28u2002·u2002drug absorption across the Caco-2 cells (Figure 1). The receiver chamber (185 mL) contained HBSS supplemented with 4% bovine serum albumin (pH 7.4). Digestion studies, in the standard glass reaction vessel or in the upper chamber of the lipolysis-permeation setup, were performed as described previously with minor modifications.18

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  • Availability of polyphenols in fruit beverages subjected ...

    2009-6-1u2002·u20022.2.In vitro gastrointestinal digestion. Fruit beverages were subjected to successive in vitro gastric and intestinal digestion as described previously (Perales, Barberá, Lagarda, & Farré, 2005).Briefly, fruit beverages (80 g) were digested with a mixture of pepsin–HCl (pH 2.0) for 2 h to simulate gastric digestion, followed by a 2 h intestinal digestion with pancreatin and bile salts (pH ...

    Get Price
  • Bioactive Protein Hydrolysate Obtained from Canned

    2020-6-14u2002·u2002Abstract A sardine protein hydrolysate (SPH) was prepared by hydrolysis of sarcoplasmic proteins from canned sardine by-product with proteases from brewer's spent yeast (BSY). The SPH presented in vitro angiotensin converting enzyme-inhibitory (ACE-I) and ferric reducing antioxidant potential (FRAP). However, the challenge before in vivo efficacy studies is to understand the impact of ...

    Get Price
  • Fetal Bovine Serum (FBS): Past-Present-Future. - Free ...

    2018-1-1u2002·u20021.1 Workshops on the replacement of FBS. This is the report of the 3rd workshop devoted to the replacement of fetal bovine serum (FBS) in cell and tissue culture methods. The 1st workshop, held in Utrecht, The Netherlands, in 2003 (van der Valk et al., 2004) was initiated after publication of a comprehensive report on pain and distress ...

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  • Potential prebiotic effect of fruit and vegetable ...

    2020-11-1u2002·u2002The fruit and vegetable byproducts flour (FVBP flour) was obtained from solid byproducts of fruit and vegetable processing for an isotonic drink development, as described by Ferreira et al. (2015).The solid byproducts were dried in an oven at 65 °C for 6 h and then ground for 5 min for obtained the FVBP flour with an average particle size of 350 μm (Andrade et al., 2016, Ferreira et al., 2015).

    Get Price
  • Maturation of the preterm gastrointestinal tract can be ...

    2021-6-17u2002·u2002Functionality of the gastrointestinal tract is essential for growth and development of newborns. Preterm infants have an immature gastrointestinal tract, which is a …

    Get Price
  • Antarctic krill derived peptide as a nanocarrier of iron ...

    2020-8-1u2002·u2002Antarctic krill (Euphausia superba) might have specific peptides that chelate iron ions and are iron carriers.In this study, iron-binding peptides were isolated from Antarctic krill using enzymatic (trypsin) hydrolysis and immobilized metal affinity chromatography (IMAC); the binding mode and surface morphology of Antarctic krill peptides (AKP)-iron complexes as well as their iron delivery ...

    Get Price
  • Selenium Bioaccessibility and Bioavailability in Se ...

    2013-2-9u2002·u2002Selenium Analysis. For verification of the information supplied by the producer, total Se concentration in the FS samples was measured by ICP-MS (820-MS, Varian) after acidic mineralization (50 % nitric acid (v/v)) in microwave (X-PRESS; CEM Corporation, Matthews, NC, USA).Isotopes 77 and 78 of Se were measured with use of 90 ml min −1 of H 2 as reaction gas to remove interferences on …

    Get Price
  • Intestinal NHE8 is highly expressed in goblet cells and ...

    2015-10-21u2002·u2002the intestine plays an important role in digestion and absorption, the mucus lining the epithelium represents a pivotal function in mucosal protection. Goblet cells are scattered in both the crypts and among enterocytes, and they secrete an important component of mucus, mucin. We have reported that sodium/hydrogen exchanger (NHE) 8 is

    Get Price
  • Enhancement of D-chiro-inositol transport across ...

    2020-10-16u2002·u2002gastrointestinal digestion, were tested as possi - ble modulators of the intestinal permeability of D-chiro-inositol. RESULTS: The absorption of this stereoiso-mer was relatively low and presumably due to passive diffusion, while it was greatly enhanced by the presence of α-Lactalbumin digest. α-Lac-talbumin peptides induced an increase in para-

    Get Price
  • Lipolysis-Permeation Setup for Simultaneous Study of ...

    2019-3-28u2002·u2002drug absorption across the Caco-2 cells (Figure 1). The receiver chamber (185 mL) contained HBSS supplemented with 4% bovine serum albumin (pH 7.4). Digestion studies, in the standard glass reaction vessel or in the upper chamber of the lipolysis-permeation setup, were performed as described previously with minor modifications.18

    Get Price
  • Availability of polyphenols in fruit beverages subjected ...

    2009-6-1u2002·u20022.2.In vitro gastrointestinal digestion. Fruit beverages were subjected to successive in vitro gastric and intestinal digestion as described previously (Perales, Barberá, Lagarda, & Farré, 2005).Briefly, fruit beverages (80 g) were digested with a mixture of pepsin–HCl (pH 2.0) for 2 h to simulate gastric digestion, followed by a 2 h intestinal digestion with pancreatin and bile salts (pH ...

    Get Price
  • Bioactive Protein Hydrolysate Obtained from Canned

    2020-6-14u2002·u2002Abstract A sardine protein hydrolysate (SPH) was prepared by hydrolysis of sarcoplasmic proteins from canned sardine by-product with proteases from brewer's spent yeast (BSY). The SPH presented in vitro angiotensin converting enzyme-inhibitory (ACE-I) and ferric reducing antioxidant potential (FRAP). However, the challenge before in vivo efficacy studies is to understand the impact of ...

    Get Price
  • Fetal Bovine Serum (FBS): Past-Present-Future. - Free ...

    2018-1-1u2002·u20021.1 Workshops on the replacement of FBS. This is the report of the 3rd workshop devoted to the replacement of fetal bovine serum (FBS) in cell and tissue culture methods. The 1st workshop, held in Utrecht, The Netherlands, in 2003 (van der Valk et al., 2004) was initiated after publication of a comprehensive report on pain and distress ...

    Get Price
  • Potential prebiotic effect of fruit and vegetable ...

    2020-11-1u2002·u2002The fruit and vegetable byproducts flour (FVBP flour) was obtained from solid byproducts of fruit and vegetable processing for an isotonic drink development, as described by Ferreira et al. (2015).The solid byproducts were dried in an oven at 65 °C for 6 h and then ground for 5 min for obtained the FVBP flour with an average particle size of 350 μm (Andrade et al., 2016, Ferreira et al., 2015).

    Get Price
  • Maturation of the preterm gastrointestinal tract can be ...

    2021-6-17u2002·u2002Functionality of the gastrointestinal tract is essential for growth and development of newborns. Preterm infants have an immature gastrointestinal tract, which is a …

    Get Price
  • Antarctic krill derived peptide as a nanocarrier of iron ...

    2020-8-1u2002·u2002Antarctic krill (Euphausia superba) might have specific peptides that chelate iron ions and are iron carriers.In this study, iron-binding peptides were isolated from Antarctic krill using enzymatic (trypsin) hydrolysis and immobilized metal affinity chromatography (IMAC); the binding mode and surface morphology of Antarctic krill peptides (AKP)-iron complexes as well as their iron delivery ...

    Get Price
  • Selenium Bioaccessibility and Bioavailability in Se ...

    2013-2-9u2002·u2002Selenium Analysis. For verification of the information supplied by the producer, total Se concentration in the FS samples was measured by ICP-MS (820-MS, Varian) after acidic mineralization (50 % nitric acid (v/v)) in microwave (X-PRESS; CEM Corporation, Matthews, NC, USA).Isotopes 77 and 78 of Se were measured with use of 90 ml min −1 of H 2 as reaction gas to remove interferences on …

    Get Price
  • Dietary fructose improves intestinal cell survival and ...

    2021-8-18u2002·u2002HCT116 and DLD1 cells were obtained from ATCC and cultured in DMEM (Corning, 10-013-CV) supplemented with 10% dialysed fetal bovine serum (dFBS) and 100 U ml −1 penicillin and 100 μg ml −1 ...

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  • Enhancement of D-chiro-inositol transport across ...

    2020-10-16u2002·u2002gastrointestinal digestion, were tested as possi - ble modulators of the intestinal permeability of D-chiro-inositol. RESULTS: The absorption of this stereoiso-mer was relatively low and presumably due to passive diffusion, while it was greatly enhanced by the presence of α-Lactalbumin digest. α-Lac-talbumin peptides induced an increase in para-

    Get Price
  • Modulatory Role of PYY in Transport and Metabolism of ...

    2012-7-23u2002·u2002Background Gastrointestinal peptides are involved in modulating appetite. Other biological functions attributed to them include the regulation of lipid homeostasis. However, data concerning PYY remain fragmentary. The objectives of the study were: (i) To determine the effect of PYY on intestinal transport and synthesis of cholesterol, the biogenesis of apolipoproteins …

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  • Chondroprotective Properties of Human-Enriched Serum ...

    Polyphenols are widely acknowledged for their health benefits, especially for the prevention of inflammatory and age-related diseases. We previously demonstrated that hydroxytyrosol (HT) and procyanidins (PCy), alone or in combination, drive preventive anti-osteoathritic effects in vivo. However, the lack of sufficient clinical evidences on the relationship between dietary …

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  • Mechanisms of oral absorption improvement for insoluble ...

    oral absorption improvement for insoluble drugs by the combination of phospholipid complex and SNEDDS, Drug Delivery, 26:1, 1155-1166, DOI: 10.1080/10717544.2019.1686086 To link to …

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  • Circulating Ara h 6 as a marker of peanut protein ...

    Ara h 6, a peanut 2S albumin, is a small and compact protein which is highly resistant to gastrointestinal digestion and is able to cross the intestinal epithelial barrier in a Caco‐2 cell model. 25-27 It is a major allergenic determinant of peanut allergy, 28, 29 which is the most common cause of food‐related anaphylaxis in Europe. 30, 31 ...

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  • Evaluation of In Vitro - Hindawi

    2019-7-31u2002·u2002e fermentation process has been shown to improve the antioxidant properties of plants and vegetables [, ]. More speci cally, fermentation increases the phenolic content of plant products [ ]andapositivecorrelation between the polyphenols and antioxidant activities of herbs has been reported [ ]. In parallel, fermentation can also

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  • Structural alterations of the intestinal epithelial ...

    2015-3-10u2002·u2002Functional and morphological alterations of the intestinal epithelial barrier (IEB) have been consistently reported in digestive disorders such as irritable bowel syndrome and inflammatory bowel disease. There is mounting evidence that Parkinson's disease (PD) is not only a brain disease but also a digestive disorder. Gastrointestinal involvement is a frequent and early …

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  • Canine colostrum - Vet Focus

    2021-3-19u2002·u2002In addition, the quantity of average colostrum that must be ingested for satisfactory immunity is 1.3 mL per 100 g of puppy body weight (assuming the puppy's IgG serum levels reach 2.3 g/L, with a digestive absorption rate of 40%, a hematocrit of 35%, and IgG levels in the colostrum of 20 g/L).

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  • The Influence of Micronutrients in Cell Culture: A ...

    Micronutrients, including minerals and vitamins, are indispensable to DNA metabolic pathways and thus are as important for life as macronutrients. Without the proper nutrients, genomic instability compromises homeostasis, leading to chronic diseases and certain types of cancer. Cell-culture media try to mimic the in vivo environment, providing in vitro models used to infer cells ...

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  • Intestinal NHE8 is highly expressed in goblet cells and ...

    2015-10-21u2002·u2002the intestine plays an important role in digestion and absorption, the mucus lining the epithelium represents a pivotal function in mucosal protection. Goblet cells are scattered in both the crypts and among enterocytes, and they secrete an important component of mucus, mucin. We have reported that sodium/hydrogen exchanger (NHE) 8 is

    Get Price
  • Enhancement of D-chiro-inositol transport across ...

    2020-10-16u2002·u2002gastrointestinal digestion, were tested as possi - ble modulators of the intestinal permeability of D-chiro-inositol. RESULTS: The absorption of this stereoiso-mer was relatively low and presumably due to passive diffusion, while it was greatly enhanced by the presence of α-Lactalbumin digest. α-Lac-talbumin peptides induced an increase in para-

    Get Price
  • Lipolysis-Permeation Setup for Simultaneous Study of ...

    2019-3-28u2002·u2002drug absorption across the Caco-2 cells (Figure 1). The receiver chamber (185 mL) contained HBSS supplemented with 4% bovine serum albumin (pH 7.4). Digestion studies, in the standard glass reaction vessel or in the upper chamber of the lipolysis-permeation setup, were performed as described previously with minor modifications.18

    Get Price
  • Availability of polyphenols in fruit beverages subjected ...

    2009-6-1u2002·u20022.2.In vitro gastrointestinal digestion. Fruit beverages were subjected to successive in vitro gastric and intestinal digestion as described previously (Perales, Barberá, Lagarda, & Farré, 2005).Briefly, fruit beverages (80 g) were digested with a mixture of pepsin–HCl (pH 2.0) for 2 h to simulate gastric digestion, followed by a 2 h intestinal digestion with pancreatin and bile salts (pH ...

    Get Price
  • Bioactive Protein Hydrolysate Obtained from Canned

    2020-6-14u2002·u2002Abstract A sardine protein hydrolysate (SPH) was prepared by hydrolysis of sarcoplasmic proteins from canned sardine by-product with proteases from brewer's spent yeast (BSY). The SPH presented in vitro angiotensin converting enzyme-inhibitory (ACE-I) and ferric reducing antioxidant potential (FRAP). However, the challenge before in vivo efficacy studies is to understand the impact of ...

    Get Price
  • Fetal Bovine Serum (FBS): Past-Present-Future. - Free ...

    2018-1-1u2002·u20021.1 Workshops on the replacement of FBS. This is the report of the 3rd workshop devoted to the replacement of fetal bovine serum (FBS) in cell and tissue culture methods. The 1st workshop, held in Utrecht, The Netherlands, in 2003 (van der Valk et al., 2004) was initiated after publication of a comprehensive report on pain and distress ...

    Get Price
  • Potential prebiotic effect of fruit and vegetable ...

    2020-11-1u2002·u2002The fruit and vegetable byproducts flour (FVBP flour) was obtained from solid byproducts of fruit and vegetable processing for an isotonic drink development, as described by Ferreira et al. (2015).The solid byproducts were dried in an oven at 65 °C for 6 h and then ground for 5 min for obtained the FVBP flour with an average particle size of 350 μm (Andrade et al., 2016, Ferreira et al., 2015).

    Get Price
  • Maturation of the preterm gastrointestinal tract can be ...

    2021-6-17u2002·u2002Functionality of the gastrointestinal tract is essential for growth and development of newborns. Preterm infants have an immature gastrointestinal tract, which is a …

    Get Price
  • Antarctic krill derived peptide as a nanocarrier of iron ...

    2020-8-1u2002·u2002Antarctic krill (Euphausia superba) might have specific peptides that chelate iron ions and are iron carriers.In this study, iron-binding peptides were isolated from Antarctic krill using enzymatic (trypsin) hydrolysis and immobilized metal affinity chromatography (IMAC); the binding mode and surface morphology of Antarctic krill peptides (AKP)-iron complexes as well as their iron delivery ...

    Get Price
  • Selenium Bioaccessibility and Bioavailability in Se ...

    2013-2-9u2002·u2002Selenium Analysis. For verification of the information supplied by the producer, total Se concentration in the FS samples was measured by ICP-MS (820-MS, Varian) after acidic mineralization (50 % nitric acid (v/v)) in microwave (X-PRESS; CEM Corporation, Matthews, NC, USA).Isotopes 77 and 78 of Se were measured with use of 90 ml min −1 of H 2 as reaction gas to remove interferences on …

    Get Price
  • Early-Childhood Membranous Nephropathy Due to

    2019-9-10u2002·u2002Heat treatment of bovine serum albumin denatures the protein and results in reduced proteolysis 24 in the relatively high pH (3 or 4) of infants' stomach as compared with that of adults (pH 2). 25 ...

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  • Nanoscale Delivery of Resveratrol towards Enhancement

    2017-5-28u2002·u2002Caco-2 cells were grown in DMEM supplemented with 10% fetal bovine serum, 1% fungizone, 1% Pen Strep at 37 C, and 5% CO 2. Cells were supplied with a fresh medium every 2 days and subcultured by treatment with 0.25% trypsin–EDTA when …

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  • Feeding the Newborn Dairy Calf - Penn State Extension

    2017-9-4u2002·u2002Whey passes directly into the small intestine for absorption and/or digestion within 10 minutes after feeding. From the small intestine, immunoglobulins can be absorbed into the calf's blood stream. Again, the newborn's limited digestive capacity aids the calf by enabling rapid absorption of the immunoglobulins that it needs.

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  • MicroRNA-132 reverses cisplatin resistance and

    2019-5-17u2002·u2002containing 10% fetal bovine serum (FBS; Gibco, Rockville, MD, USA), 1% penicillin-streptomy-cin dual antibody solution, and 1% nonessential amino acids, and placed in an incubator with 5% CO 2 at 37°C. Transfection of MiR-132 MiR-132 mimic and miR-132 mimic negative control (NC) were purchased from GenePharma (Shanghai, China). SKOV3 and SKOV3/DDP

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  • Effects of silver nanoparticles and ions on a co-culture ...

    2016-2-17u2002·u2002The increased incorporation of silver nanoparticles (Ag NPs) into consumer products makes the characterization of potential risk for humans and other organisms essential. The oral route is an important uptake route for NPs, therefore the study of the gastrointestinal tract in respect to NP uptake and toxicity is very timely. The aim of the present study was to evaluate the effects of Ag NPs ...

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  • VWR, Part of Avantor - Chemicals and Laboratory Scientific ...

    VWR, Part of Avantor - Chemicals and Laboratory Scientific Supplies. VWR, Part of Avantor. Carousel. Moving Forward. Together. So much has changed during this unprecedented time, except your ability to count on Avantor. We continue to set science in motion, and know safety is …

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  • Two million children innoculated with BSE vaccines

    2001-2-12u2002·u20024. J have a measles vaccine using bovine serum from the UK. There are 440,000 units of stock. They have also got MMR using bovine serum from the UK. 5. K have influenza, rubella, measles, MMR vaccines likely to be used in children. Of those they think that only MMR contains bovine material which is probably a French origin. 6.

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  • Inhibition of KIT RNAi mediated with adenovirus in ...

    Inhibition of KIT RNAi mediated with adenovirus in gastrointestinal stromal tumor xenograft. Inhibition of KIT RNAi mediated with adenovirus in gastrointestinal stromal tumor xenograft. Inhibition of KIT RNAi mediated with adenovirus in gastrointestinal stromal tumor xenograft

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  • Patient Education on Blood, Urine, and ... - Lab Tests Online

    Lab Tests Online (LTO) is an award-winning health information web resource designed to help patients & caregivers understand the many lab tests that are a vital part of medical care. Lab and medical professionals develop and review all content, including articles on lab tests, conditions/diseases, screenings & more. The site is produced by AACC, a global scientific and medical professional ...

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  • UpToDate

    2021-9-23u2002·u2002{{configCtrl2.info.metaDescription}}

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  • (PDF) In vitro cellular models of human hepatic fatty acid ...

    2017-12-1u2002·u2002Storage and synthesis of triacylglycerol (TAG) in Huh7 and HepG2 cells. Cells were cultured in either 2% human serum (HS) or 10% fetal bovine serum (FBS) …

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  • Salivary and Gut Microbiomes Play a Significant Role in in ...

    2018-11-7u2002·u2002The release of a toxicant from a food matrix during the gastrointestinal digestion is a crucial determinant of the toxicant's oral bioavailability. We present a modified setup of the human simulator of the gut microbial ecosystem (SHIME), with four sequential gastrointestinal reactors (oral, stomach, small intestine, and colon), including the salivary and colonic microbiomes.

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  • Gastrointestinal cancer cells treatment with bevacizumab ...

    activity. Interestingly, VEGF inhibition with bevacizumab (100μg/ml) increased hTERT expression 42.3% in AGS, 94.1% in Caco2, and 52.5% in HepG2/C3A, and increased telo-

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  • Protective Effects of Intestinal Trefoil Factor against ...

    2017-12-31u2002·u2002Therefore, we performed the initial digestion at 37 AdegC and used the residual tissue for digestion at 4 AdegC overnight to achieve higher cell viability and improve the yield. In the present study, we used 5% fetal bovine serum to reduce the …

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  • Frontiers

    The description of 'serum sickness' more than a century ago in humans transfused with animal sera eventually led to identification of a class of human antibodies directed against glycans terminating in the common mammalian sialic acid N-Glycolylneuraminic acid (Neu5Gc), hereafter called 'Neu5Gc-glycans.' The detection of such glycans in malignant and fetal human tissues initially ...

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  • Frontiers

    Human milk is uniquely optimized for the needs of the developing infant. Its composition is complex and dynamic, driven primarily by maternal genetics, and to a lesser extent by diet and environment. One important component that is gaining attention is the milk fat globule (MFG). The MFG is composed of a triglyceride-rich core surrounded by a tri-layer membrane, also known as the milk …

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  • Crocin from Crocus sativus possesses significant anti ...

    2007-9-1u2002·u2002Non-cancer young adult mouse colon (YAMC) cells (RPMI 1640) were obtained from Prof. E.B. Chang in the Digestive Diseases Research Core Center at the University of Chicago. For cancer cell culture, indicated medium containing 10% fetal bovine serum and penicillin/streptomycin was placed in a humidified atmosphere with 5% CO 2 at 37 °C.

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  • The Administration Matrix Modifies the Beneficial ...

    2020-8-26u2002·u2002Consumption of dairy products is one of the most natural ways to introduce probiotics. However, the beneficial effects of the probiotics might depend on the administration form. The aim of this study was to investigate the beneficial properties of two probiotic strains: Bifidobacterium animalis subsp. lactis (BB-12) and Lactobacillus acidophilus (LA-5) in different administration …

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  • Production and purification of mannan oligosaccharide with ...

    2021-11-1u2002·u2002Background Mannanan oligosaccharide (MOS) is well-known as effective supplement food for livestock to increase their nutrients absorption and health status, but the structure and identification of bioactive MOS remain unclear. In this study, MOS production was accomplished, using enzymatic hydrolysis of pretreated coconut meal substrate with recombinant mannanase.

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  • In Vitro Assessment of Cadmium Bioavailability in Chinese ...

    The minimum concentration of cadmium (Cd), by Chinese cabbage grown on Cd contaminated soils that can initiate toxicity in human liver cells using in vitro digestion coupled with Caco-2/HL-7702 cell models was studied. Cadmium bioaccessibility in the gastric phase for yellow soil (YS) cabbage (40.84%) and calcareous soil (CS) cabbage (21.54%) was significantly higher than small …

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