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  • Bovine Serum Albumin fatty acid free, low endotoxin ...

    Bovine serum albumin (BSA) is a water-soluble serum protein, 583 amino acids in length, with a calculated molecular weight of 66,430 Daltons. Six a-helices form its three homologous domains. Depending on pH, bovine serum albumin undergoes reversible conformational isomerization. BSA native structure becomes reactive and flexible on heating.

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  • Plant-based and cell-based approaches to meat production

    2020-12-8u2002·u2002Fetal bovine serum is a common additive to cell culture media and it provides essential growth factors for mammalian cell culture. In 2003, it was estimated that blood from 1,000,000 bovine fetuses was harvested to generate the annual production of 500,000 liters of fetal bovine serum 81. Serum-free alternatives include co-culture approaches or ...

    Get Price
  • The Effect of Oleic and Palmitic Acid on Induction of ...

    2020-4-2u2002·u2002medium without fetal bovine serum with the addition of 1) oleic acid (OA) at a concentration of 0.125, 0.25, 0.5, 0.75, 1 and 2 mmol/l, 2) palmitic acid (PA) at the same concentrations and 3) combination of OA and PA at

    Get Price
  • Plant-based and cell-based approaches to meat production ...

    2020-12-8u2002·u2002In 2003, it was estimated that blood from 1,000,000 bovine fetuses was harvested to generate the annual production of 500,000 liters of fetal bovine serum 81. …

    Get Price
  • Effects of tumour necrosis factor alpha (TNF(x) on glucose

    2017-8-29u2002·u2002mented with 10 % fetal bovine serum and inoculated into 35- mm or 100-ram dishes at a density of 30,000 to 50,000 cells per cm 2. After a 16-h incubation period, cultures were repeatedly washed with PBS to remove non-adhering material and re-fed with a serum-free medium consisting of Dulbecco's Modified

    Get Price
  • Bioprocess development for scalable production of ...

    2020-6-22u2002·u2002Upon thawing, the cells were cultured in T-flasks using a growth medium comprising of α-modified Eagle's medium (1 g/L glucose; Lonza, UK) supplemented with 10% (vol/vol) fetal bovine serum (Sigma-Aldrich, UK), 1 ng/ml bFGF (PeproTech, UK) and 2 mM UltraGlutamine (Lonza). bASCs between passage numbers 2 and 10 were used for the planar study ...

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  • Meat no longer requires animal slaughter

    2021-9-28u2002·u2002Growing meat directly from animal cells offers a way of squaring the circle, while also satisfying the moral demands of consumers uneasy about factory farming and animal slaughter. But it …

    Get Price
  • Corning

    Corning Incorporated is a global-leading innovator in materials science, with 170 years of life-changing inventions and category-defining products.

    Get Price
  • Bovine Serum Albumin fatty acid free, low endotoxin ...

    Bovine serum albumin (BSA) is a water-soluble serum protein, 583 amino acids in length, with a calculated molecular weight of 66,430 Daltons. Six a-helices form its three homologous domains. Depending on pH, bovine serum albumin undergoes reversible conformational isomerization. BSA native structure becomes reactive and flexible on heating.

    Get Price
  • Plant-based and cell-based approaches to meat production

    2020-12-8u2002·u2002Fetal bovine serum is a common additive to cell culture media and it provides essential growth factors for mammalian cell culture. In 2003, it was estimated that blood from 1,000,000 bovine fetuses was harvested to generate the annual production of 500,000 liters of fetal bovine serum 81. Serum-free alternatives include co-culture approaches or ...

    Get Price
  • The Effect of Oleic and Palmitic Acid on Induction of ...

    2020-4-2u2002·u2002medium without fetal bovine serum with the addition of 1) oleic acid (OA) at a concentration of 0.125, 0.25, 0.5, 0.75, 1 and 2 mmol/l, 2) palmitic acid (PA) at the same concentrations and 3) combination of OA and PA at

    Get Price
  • Plant-based and cell-based approaches to meat

    2020-12-8u2002·u2002In 2003, it was estimated that blood from 1,000,000 bovine fetuses was harvested to generate the annual production of 500,000 liters of fetal bovine serum 81. …

    Get Price
  • Effects of tumour necrosis factor alpha (TNF(x) on glucose

    2017-8-29u2002·u2002mented with 10 % fetal bovine serum and inoculated into 35- mm or 100-ram dishes at a density of 30,000 to 50,000 cells per cm 2. After a 16-h incubation period, cultures were repeatedly washed with PBS to remove non-adhering material and re-fed with a serum-free medium consisting of Dulbecco's Modified

    Get Price
  • Frontiers

    2020-2-7u2002·u2002The global population, 7.3 billion today, is expected to surpass 9 billion by 2050. The Food and Agriculture Organization (FAO) has forecast that in 2050, 70% more food will be needed to fulfill the demand of the growing population, which is a great challenge due to resource and arable land limitations. Even if meat consumption is decreasing in ...

    Get Price
  • Bioprocess development for scalable production of ...

    2020-6-22u2002·u2002Upon thawing, the cells were cultured in T-flasks using a growth medium comprising of α-modified Eagle's medium (1 g/L glucose; Lonza, UK) supplemented with 10% (vol/vol) fetal bovine serum (Sigma-Aldrich, UK), 1 ng/ml bFGF (PeproTech, UK) and 2 mM UltraGlutamine (Lonza). bASCs between passage numbers 2 and 10 were used for the planar study ...

    Get Price
  • Meat no longer requires animal slaughter

    2021-9-28u2002·u2002Growing meat directly from animal cells offers a way of squaring the circle, while also satisfying the moral demands of consumers uneasy about …

    Get Price
  • Corning

    Corning Incorporated is a global-leading innovator in materials science, with 170 years of life-changing inventions and category-defining products.

    Get Price
  • A protocol for the isolation and cultivation of brown bear ...

    Biopsies were taken from 8 captive male (N = 6) and female (N = 2) brown bears, ages 2-12 years. Plastic adherent, fibroblast-like cells were proliferated and subsequently cryopreserved or differentiated. Differentiation conditions were optimized with respect to fetal bovine serum content and time spent in differentiation medium.

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  • USDA APHIS

    2021-4-13u2002·u2002Diets for Laboratory or Zoo Animals. Fetal Bovine Serum Collected in the United States and/or Canada - August 2018 (pdf 45kb) Forwarding Certification for Animal Products Legally Imported into the United States for Further Export to Canada - December 2017 (pdf 65kb) Insect Meal for Pet and Ornamental Fish Food or Livestock Feed.

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  • Role of Aramchol in Steatohepatitis and Fibrosis in Mice

    2017-11-28u2002·u2002mented with 10% fetal bovine serum (FBS) in a 5% CO 2–95% air incubator at 378C. After 2 hours of attachment, the culture medium was removed and replaced by serum-free MEM. After 3 hours in cul-ture, the medium was replaced by serum-free MEM or MCD medium, with or without Aramchol (10 lM), and cultured for an additional 48 hours. The human

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  • PalmitoleateReversesHighFat-inducedProinflammatory ...

    2020-9-1u2002·u2002fibroblasts were grown to confluence in low glucose DMEM (Wisent) supplemented with 10% fetal bovine serum and 1 min, and protein concentration was determined in superna-antibiotic-antimycotic(Wisent)andthenwashedwithPBSand serum-starved for 10 days. Medium was then collected and fil-ter-sterilized, aliquoted, and stored at 20 °C as L929 condi-

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  • Richard A. Holt, Jim Bertolini,* Kenneth Cain** and Amy

    2021-8-22u2002·u2002of 1 to 5% fetal calf serum to cytophaga agar. Also, the addition of 0.2 to 0.5% sterile skim milk (added just prior to pouring plates) or 0.5% bovine serum albumin (filter sterilized) to TYES may promote heavier and more rapid growth. Growth and maintenance of . F. psychrophilum. has been recently reviewed (Cain and LaFrentz 2007).

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  • Serum: Advanced Wavetable Synthesizer - Xfer Records

    Serum will let you stack a single oscillator to use up to 16 voices. Each of the wavetable oscillators have a number of unison advanced parameters. Stack settings allow for note (e.g. octave) layering to get a fuller sound for a single note-press. Set the unison voices to all have their unique waveform at once with the Unison WT Pos setting, or ...

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  • Technological, Regulatory, and Ethical Aspects of In Vitro ...

    2019-6-13u2002·u2002Defined as meat cultured in a laboratory within a bioreactor under controlled artificial conditions, in vitro meat is a relatively recent area that has opened a whole universe of possibilities and opportunities for the meat sector. With improved chemical and microbial safety and varied options, in vitro meat has been proposed as a green, healthy, environmentally friendly, and nutritionally ...

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  • About Us - Sigma-Aldrich

    Life Science. We are MilliporeSigma, a global science and technology company determined to solve the toughest problems in life science. Our tools, services, and digital platforms empower scientists and engineers at every stage, helping to deliver scientific breakthroughs. Learn more about us.

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  • Cultured meat: state of the art and future

    2018-3-19u2002·u2002Cultured meat or in vitro meat offers a safe and disease-free way forward to meet increasing meat requirement without involving animal sacrifices and at the same time, reducing greenhouse emissions, as compared to conventional meat. However, its cost, scale-up, public neophobia and technophobia, and incomplete understanding of health benefits, limits its commercial viability. This …

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  • ADIPOQ - Adiponectin precursor - Bos taurus (Bovine ...

    2005-9-27u2002·u2002Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as ...

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  • Bovine Serum Albumin fatty acid free, low endotoxin ...

    Bovine serum albumin (BSA) is a water-soluble serum protein, 583 amino acids in length, with a calculated molecular weight of 66,430 Daltons. Six a-helices form its three homologous domains. Depending on pH, bovine serum albumin undergoes reversible conformational isomerization. BSA native structure becomes reactive and flexible on heating.

    Get Price
  • Plant-based and cell-based approaches to meat production

    2020-12-8u2002·u2002Fetal bovine serum is a common additive to cell culture media and it provides essential growth factors for mammalian cell culture. In 2003, it was estimated that blood from 1,000,000 bovine fetuses was harvested to generate the annual production of 500,000 liters of fetal bovine serum 81. Serum-free alternatives include co-culture approaches or ...

    Get Price
  • The Effect of Oleic and Palmitic Acid on Induction of ...

    2020-4-2u2002·u2002medium without fetal bovine serum with the addition of 1) oleic acid (OA) at a concentration of 0.125, 0.25, 0.5, 0.75, 1 and 2 mmol/l, 2) palmitic acid (PA) at the same concentrations and 3) combination of OA and PA at

    Get Price
  • Plant-based and cell-based approaches to meat

    2020-12-8u2002·u2002In 2003, it was estimated that blood from 1,000,000 bovine fetuses was harvested to generate the annual production of 500,000 liters of fetal bovine serum 81. Serum-free alternatives include co ...

    Get Price
  • Effects of tumour necrosis factor alpha (TNF(x) on glucose

    2017-8-29u2002·u2002mented with 10 % fetal bovine serum and inoculated into 35- mm or 100-ram dishes at a density of 30,000 to 50,000 cells per cm 2. After a 16-h incubation period, cultures were repeatedly washed with PBS to remove non-adhering material and re-fed with a serum-free medium consisting of Dulbecco's Modified

    Get Price
  • Frontiers

    2020-2-7u2002·u2002The global population, 7.3 billion today, is expected to surpass 9 billion by 2050. The Food and Agriculture Organization (FAO) has forecast that in 2050, 70% more food will be needed to fulfill the demand of the growing population, which is a great challenge due to resource and arable land limitations. Even if meat consumption is decreasing in ...

    Get Price
  • Bioprocess development for scalable production of ...

    2020-6-22u2002·u2002Upon thawing, the cells were cultured in T-flasks using a growth medium comprising of α-modified Eagle's medium (1 g/L glucose; Lonza, UK) supplemented with 10% (vol/vol) fetal bovine serum (Sigma-Aldrich, UK), 1 ng/ml bFGF (PeproTech, UK) and 2 mM UltraGlutamine (Lonza). bASCs between passage numbers 2 and 10 were used for the planar study ...

    Get Price
  • Meat no longer requires animal slaughter

    2021-9-28u2002·u2002Growing meat directly from animal cells offers a way of squaring the circle, while also satisfying the moral demands of consumers uneasy about …

    Get Price
  • Corning

    Corning Incorporated is a global-leading innovator in materials science, with 170 years of life-changing inventions and category-defining products.

    Get Price
  • Jingyi Ding Nov 16 BIO-320-003-2017F lab

    2018-10-10u2002·u2002of FBS is the globular protein called bovine serum albumin (BSA), such proteins have a rich variety and high level of nutrients which allows FBS maintains cultured cells in media to survive, grow and divide. (Carlo, 2002) By adding a different amount of camptothecin and FBS into the cell cultures media caused the diversity of cells growth

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  • Serum: Advanced Wavetable Synthesizer - Xfer Records

    Serum will let you stack a single oscillator to use up to 16 voices. Each of the wavetable oscillators have a number of unison advanced parameters. Stack settings allow for note (e.g. octave) layering to get a fuller sound for a single note-press. Set the unison voices to all have their unique waveform at once with the Unison WT Pos setting, or ...

    Get Price
  • Chylomicron-specific Enhancement of Acylation Stimulating ...

    2020-11-12u2002·u2002Essentially fatty acid-free bovine serum albumin, collagenase type II, lipid substrate, cholesterol, and human transthyretin (TTR) were from Sigma. All tissue culture medium, Dulbecco's phosphate-buffered saline * This work was supported by Medical Research Council of Canada Grant MT-13716 (to K. C.). The costs of publication of this article were

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  • MilliporeSigma

    MilliporeSigma. Our purpose is to solve the toughest problems in life science by collaborating with the global scientific community – and through that, we aim to accelerate access to better health for people everywhere. We provide scientists and engineers with best-in-class lab materials, technologies and services.

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  • iHerb.com - Vitamins, Supplements & Natural Health Products

    Chocolove, Extreme Dark Chocolate, 88% Cocoa Content, 3.2 oz (90 g) 3706. $2.94. Add to cart. Coconut Secret, The Original Coconut Aminos, Soy-Free Alternative to Soy Sauce, 8 fl oz (237 ml) 7501. $5.33. Add to cart. Neutrogena, The Anti-Residue Shampoo, All Hair Types, 6 …

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  • Fatty acid profile and proliferation of bovine blood ...

    2012-6-5u2002·u2002Conjugated linoleic acids (CLA) are in focus of dairy cattle research because of its milk fat reducing effects. Little is known about the impact of CLA on immune function in dairy cows. Therefore, in the present study we investigated the effects of a long term supplementation of dairy cows with CLA on the fatty acid profile of peripheral blood mononuclear cells (PBMC) and their …

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  • Challenges and possibilities for bio-manufacturing ...

    2020-3-1u2002·u2002Therefore, it is important to develop efficient, safe, and large-scale production of cultured meat to reduce the production costs effectively ( Arshad et al., 2017 ). One of the challenges to cultured meat production is the selection of an appropriate cell source for the animal tissue culture ( Post, 2012 ).

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  • Bioprinted Vascularized Mature Adipose Tissue with ...

    2021-6-18u2002·u2002Research Article Bioprinted Vascularized Mature Adipose Tissue with Collagen Microfibers for Soft Tissue Regeneration Fiona Louis,1 Marie Piantino,2 Hao Liu,2 Dong-Hee Kang,2 Yoshihiro Sowa,3 Shiro Kitano,1,4 and Michiya Matsusaki 1,2 1Joint Research Laboratory (TOPPAN) for Advanced Cell Regulatory Chemistry, Graduate School of Engineering, Osaka …

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  • Lab-grown meat: a rosy future? – Juan Pascual

    2019-1-2u2002·u2002The environment: It is alleged, as an axiom, that lab-grown meat will have a much smaller impact on the environment than traditional meat. According to FAO data, global livestock production emits 14.5% of total greenhouse gases, it uses high energy resources, and a …

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  • Is Lab Meat About to Hit Your Dinner Plate? – Mother Jones

    2021-8-2u2002·u2002Fetal bovine serum is a slaughterhouse byproduct (it's blood extracted from the unborn fetuses of slaughtered cows) used mainly by the biomedical …

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  • The clean meat industry is racing to ditch its reliance on ...

    2018-3-20u2002·u2002A single litre of foetal bovine serum costs between £300 and £700, and the clean meat industry is getting through buckets of the stuff every day. Mark Post, co …

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  • Challenges and possibilities for bio-manufacturing ...

    2020-3-1u2002·u2002Therefore, it is important to develop efficient, safe, and large-scale production of cultured meat to reduce the production costs effectively ( Arshad et al., 2017 ). One of the challenges to cultured meat production is the selection of an appropriate cell source for the animal tissue culture ( Post, 2012 ).

    Get Price
  • Lab Equipment and Lab Supplies

    Lab Equipment and Lab Supplies Fisher Scientific. The fishersci.com site will be undergoing scheduled maintenance from 10 a.m. to 12 p.m. ET, Saturday, October 30. We thank you for your patience during this time. Contact Customer Service for assistance at 1-800-766-7000 from 7 a.m. ET to 8 p.m. ET, Monday through Friday.

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  • Genetic Engineering and Biotechnology of Growth

    2018-9-25u2002·u2002applied in a determined period of time. The taste of bovine meat and milk treated with rBGH is not altered, but the fat content is less. 3.2 Caprine growth hormone (CHGH) For small ruminants there are studies in lactatin g goats in which the administration of rBGH increased milk production 23% and stimulated mammary gland growth more than in those

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  • Hybrid products could drive first wave of cell-based meat ...

    2020-7-23u2002·u2002Hybrid products combining meat grown from cells in bioreactors with plant-based proteins and fats could drive the first wave of cell-based product launches, predicts the founder of UK-based ...

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  • Hoxton Farms grows animal fat for meat substitutes ...

    'So far, growing fat cells has not been done cost-efficiently or at a larger scale,' he explains. Traditionally, cells are cultured in vitro using fetal bovine serum, an unethical and expensive ingredient derived from the blood of cow foetuses. It is often added to culture media where the growth factors it …

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  • Transcriptome response of enterocytes to dietary lipids ...

    2008-11-1u2002·u2002Intestine contributes to lipid homeostasis through the absorption of dietary lipids, which reach the apical pole of enterocytes as micelles. The present study aimed to identify the specific impact of these dietary lipid-containing micelles on gene expression in enterocytes. We analyzed, by microarray, the modulation of gene expression in Caco-2/TC7 cells in response to different lipid supply ...

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  • The effect of culture media on large-scale expansion and ...

    2019-8-5u2002·u2002Adipose tissue-derived mesenchymal stromal cells (ASCs) have been shown to exhibit some promising properties of their use in regenerative medicine as advanced therapy medicinal products (ATMP). However, different sources of their origin, methods of isolation, and expansion procedures cause the laboratory and clinical results difficult to compare.

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  • Culture Media for Animal Cells: An Overview

    2021-11-4u2002·u2002Serum: Serum is a natural biological fluid, and is rich in various components to support cell proliferation. The major constituents found in different types of sera are listed in Table 34.3. The most commonly used sera are calf serum (CS), fetal bovine serum (FBS), horse serum and human serum.

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  • HigherSteaks' cultured pork should be ready in three years

    2019-12-16u2002·u2002Dutch startup Meatable recently announced it has raised $10m for production of its cell-grown pork. Higher Steaks has so far raised a pre-seed round of about $200,000, and will require funding of ...

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