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  • Fetal Bovine Serum - labome.comExplore further

    2021-10-17u2002·u2002Fetal Bovine Serum. Fetal bovine serum (FBS) is the liquid fraction of clotted blood from fetal calves, depleted of cells, fibrin and clotting factors, but containing a large number of nutritional and macromolecular factors essential for cell growth. Bovine serum albumin is the major component of FBS. Growth factors in FBS are essential for the ...

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  • Biowest

    Serum Replacement. Biowest is the European leader in the collection and processing of animal sera and cell culture media, offering the widest range of sera and solutions available on the market. Biowest products are in use throughout the academic, industrial and government research sectors in fields spanning cell biology, genomics, proteomics ...

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  • EDQM Certified FBS - FBS, Fetal Bovine Serum, Animal

    Biowest is committed to the highest standards and guarantees quality, reproducibility, traceability and premium service. Biowest's market success is founded on a combination of competitive prices and a wide range of geographical origins, along with scrupulous attention to accountability and traceability and a fully vertically integrated end-to-end service in which the entire sourcing ...

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  • Isoquinoline-Based Lanthanide Complexes: Bright NIR ...

    2021-3-12u2002·u2002The relaxivity of GdL1 is doubled at 40 MHz and 298 K in fetal bovine serum (r 1 = 16.1 vs 8.5 mM –1 s –1 in HEPES buffer), due to hydrophobic interactions between the chelate and serum proteins. The isoquinoline core allows for the optimization of the optical properties of the luminescent lanthanide complexes in comparison to the pyridinic ...

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  • Pre-set extrusion bioprinting for multiscale heterogeneous ...

    2018-6-6u2002·u2002The final concentration of cell-laden collagen was 3%. For pre-gelation of collagen, the bioink (e.g., cell-laden collagen) was printed onto DMEM with 10% fetal bovine serum (FBS) and 5% penicillin/streptomycin at 37 °C for 1 h, and then cut by surgical blade and moved to a …

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  • Phospho-eEF2k (Ser366) Antibody - Cell Signaling

    IP. Western blot analysis of extracts from SW-13 cells (starved for 18 hours) treated with calf intestinal alkaline phosphatase (CIP) or 20% fetal bovine serum for 30 minutes, and extracts from HeLa cells (starved for 18 hours) treated with 200 nM TPA for 30 minutes, using Phospho-eEF2k (Ser366) Antibody (upper) or eEF2k Antibody #3692 (lower).

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  • Two million children innoculated with BSE vaccines

    2001-2-12u2002·u20024. J have a measles vaccine using bovine serum from the UK. There are 440,000 units of stock. They have also got MMR using bovine serum from the UK. 5. K have influenza, rubella, measles, MMR vaccines likely to be used in children. Of those they think that only MMR contains bovine material which is probably a French origin. 6.

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  • High-Efficiency Transduction of Primary Human ...

    2016-10-19u2002·u2002We have reported that of the 10 commonly used AAV serotype vectors, AAV6 is the most efficient in transducing primary human hematopoietic stem/progenitor cells …

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  • Monitoring Viral Infection of Mammalian Cells using ...

    HeLa cells were cultured in DMEM supplemented with 10% fetal bovine serum and penicillinstreptomycin at 37 °C in 5% CO 2. Cultures were routinely trypsinized (0.05% Trypsin-EDTA) at 80% confluency. Virus infection. For experiments, cells were plated into Corning 3904 black‑sided clear-bottom 96-well microplates at 5,000 cells per well.

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  • In vitro immunotherapy potency assays using real-time cell ...

    2018-3-2u2002·u2002To make the complete basal growth medium, the following components were added to Iscove's Modified Dulbecco's Medium: 100 units/mL recombinant human IL-2, 2.5 microgram/mL human albumin, 0.5 microgram/mL D-mannitol, and fetal bovine serum to a final concentration of 20%. Raji lymphoma cells were cultured in RPMI1640 with 10% fetal bovine serum.

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  • Specificity of RNAi, LNA and CRISPRi as loss-of-function ...

    2017-12-15u2002·u2002D6429) supplemented with 10% Fetal bovine serum (FBS, Thermo Fisher Scientific). Both cell lines 5 were obtained from American Type Culture Collection (ATCC) and were cultured at 37°C with 5% CO 2. HeLa Kyoto (EGFP-α-tubulin H2B-mCherry) cells were obtained from Jan Ellenberg (EMBL, Heidelberg) (24) and cultured in DMEM with 10% FBS (25).

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  • EasySep™ Buffer for Immunomagnetic Cell Separation ...

    Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum. Cell culture buffer ... represents an attractive therapeutic modality for potentially a wide range of clinical indications but requires uridine chemistry modification and/or tuning of the production process to prevent activation of cellular innate immune sensors and a concomitant ...

    Get Price
  • Phospho-eEF2k (Ser366) Antibody - Cell Signaling

    IP. Western blot analysis of extracts from SW-13 cells (starved for 18 hours) treated with calf intestinal alkaline phosphatase (CIP) or 20% fetal bovine serum for 30 minutes, and extracts from HeLa cells (starved for 18 hours) treated with 200 nM TPA for 30 minutes, using Phospho-eEF2k (Ser366) Antibody (upper) or eEF2k Antibody #3692 (lower).

    Get Price
  • Mesenchymal Stem Cells for Clinical Application - PubMed

    Mesenchymal Stem Cells/Multipotent Marrow Stromal Cells (MSC) are multipotent adult stem cells present in all tissues, as part of the perivascular population. As multipotent cells, MSCs can differentiate into different tissues originating from mesoderm ranging from bone and cartilage, to cardiac mus …

    Get Price
  • Webinars - Eurofins Scientific

    2021-9-29u2002·u2002During this webinar, our experts will discuss relevant biosafety testing solutions to help rapidly deliver results for the patients to receive their modified cells and help support these new therapeutic approaches in the autologous cell therapy area, including: Rapid Sterility Testing. Rapid Mycoplasma Testing.

    Get Price
  • Human SOD2 ELISA Kit (ab178012)

    Human SOD2 (Superoxide Dismutase 2) ELISA kit (ab178012) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SOD2 protein in human cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human SOD2 with 220 pg/ml sensitivity.

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  • Two million children innoculated with BSE vaccines

    2001-2-12u2002·u20024. J have a measles vaccine using bovine serum from the UK. There are 440,000 units of stock. They have also got MMR using bovine serum from the UK. 5. K have influenza, rubella, measles, MMR vaccines likely to be used in children. Of those they think that only MMR contains bovine material which is probably a French origin. 6.

    Get Price
  • Cow Parts

    2001-7-31u2002·u2002Cow feet and hooves are rendered for human and pet food, gelatin, glue, buttons, handles, lubricants, cow- heel jelly, bonemeal, soaps, the foam in fire extinguishers, and fertilizers. At first glance, these seem like lists of parts that could have very little use.

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  • Novel Coronavirus hCoV-EMC Infects Humans & Animals

    2012-12-12u2002·u2002Novel Coronavirus hCoV-EMC Infects Humans & Animals. The hCoV-EMC virus could still enter bat cells (blue) even when the ACE2 receptor was blocked by antibodies (red). Studies in monkey kidney cells (left) also showed that blocking ACE2 prevented the entry of the SARS virus (top right) but not hCoV-EMC (bottom right).

    Get Price
  • In vitro immunotherapy potency assays using real-time cell ...

    2018-3-2u2002·u2002To make the complete basal growth medium, the following components were added to Iscove's Modified Dulbecco's Medium: 100 units/mL recombinant human IL-2, 2.5 microgram/mL human albumin, 0.5 microgram/mL D-mannitol, and fetal bovine serum to a final concentration of 20%. Raji lymphoma cells were cultured in RPMI1640 with 10% fetal bovine serum.

    Get Price
  • Specificity of RNAi, LNA and CRISPRi as loss-of-function ...

    2017-12-15u2002·u2002D6429) supplemented with 10% Fetal bovine serum (FBS, Thermo Fisher Scientific). Both cell lines 5 were obtained from American Type Culture Collection (ATCC) and were cultured at 37°C with 5% CO 2. HeLa Kyoto (EGFP-α-tubulin H2B-mCherry) cells were obtained from Jan Ellenberg (EMBL, Heidelberg) (24) and cultured in DMEM with 10% FBS (25).

    Get Price
  • EasySep™ Buffer for Immunomagnetic Cell Separation ...

    Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum. Cell culture buffer ... represents an attractive therapeutic modality for potentially a wide range of clinical indications but requires uridine chemistry modification and/or tuning of the production process to prevent activation of cellular innate immune sensors and a concomitant ...

    Get Price
  • Phospho-eEF2k (Ser366) Antibody - Cell Signaling

    IP. Western blot analysis of extracts from SW-13 cells (starved for 18 hours) treated with calf intestinal alkaline phosphatase (CIP) or 20% fetal bovine serum for 30 minutes, and extracts from HeLa cells (starved for 18 hours) treated with 200 nM TPA for 30 minutes, using Phospho-eEF2k (Ser366) Antibody (upper) or eEF2k Antibody #3692 (lower).

    Get Price
  • Mesenchymal Stem Cells for Clinical Application - PubMed

    Mesenchymal Stem Cells/Multipotent Marrow Stromal Cells (MSC) are multipotent adult stem cells present in all tissues, as part of the perivascular population. As multipotent cells, MSCs can differentiate into different tissues originating from mesoderm ranging from bone and cartilage, to cardiac mus …

    Get Price
  • Webinars - Eurofins Scientific

    2021-9-29u2002·u2002During this webinar, our experts will discuss relevant biosafety testing solutions to help rapidly deliver results for the patients to receive their modified cells and help support these new therapeutic approaches in the autologous cell therapy area, including: Rapid Sterility Testing. Rapid Mycoplasma Testing.

    Get Price
  • Human SOD2 ELISA Kit (ab178012)

    Human SOD2 (Superoxide Dismutase 2) ELISA kit (ab178012) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SOD2 protein in human cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human SOD2 with 220 pg/ml sensitivity.

    Get Price
  • Two million children innoculated with BSE vaccines

    2001-2-12u2002·u20024. J have a measles vaccine using bovine serum from the UK. There are 440,000 units of stock. They have also got MMR using bovine serum from the UK. 5. K have influenza, rubella, measles, MMR vaccines likely to be used in children. Of those they think that only MMR contains bovine material which is probably a French origin. 6.

    Get Price
  • Cow Parts

    2001-7-31u2002·u2002Cow feet and hooves are rendered for human and pet food, gelatin, glue, buttons, handles, lubricants, cow- heel jelly, bonemeal, soaps, the foam in fire extinguishers, and fertilizers. At first glance, these seem like lists of parts that could have very little use.

    Get Price
  • Novel Coronavirus hCoV-EMC Infects Humans & Animals

    2012-12-12u2002·u2002Novel Coronavirus hCoV-EMC Infects Humans & Animals. The hCoV-EMC virus could still enter bat cells (blue) even when the ACE2 receptor was blocked by antibodies (red). Studies in monkey kidney cells (left) also showed that blocking ACE2 prevented the entry of the SARS virus (top right) but not hCoV-EMC (bottom right).

    Get Price
  • Fetal Bovine Serum Market 2021: Industry Size, Regions,

    2021-10-11u2002·u2002Fetal Bovine Serum Market 2021-2027 Research Report provides a detailed analysis of global fetal bovine serum industry competition, driving …

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  • General catalog 7 th edition - FBS, Fetal Bovine Serum ...

    2020-2-27u2002·u2002Serum provides nearly all the components required for both adherent and suspension cell cultures, most of which are not yet chemically defined, such as growth factors, attachment factors, transporter proteins, lipids and hormones. Fetal Bovine Serum is the most widely used supplement for in vitro mammalian cell culture.

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  • ESPA

    2021-10-3u2002·u2002There is a wide range of applications for fetal bovine serum. The most important is in the field of biopharmaceuticals and vaccines. It is used in the research, manufacture and control of human and veterinary vaccines and of drugs, many of which are at the cutting edge of drug development. Fetal bovine serum is also used extensively in research.

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  • Fetal Bovine Serum Heat Inactivated at Thomas Scientific

    Heat inactivated fetal bovine serum is for in vitro diagnostic use, not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law. Certified Fetal Bovine Sera meets USDA requirements for abattoir-sourced animals, traceability and country of origin.…

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  • Systematic investigation and in vitro biocompatibility ...

    2021-10-12u2002·u2002maintained in DMEM medium containing 10% fetal bovine serum, 100 U/ml penicillin and 100 µg/ml streptomycin, in a humidified atmosphere (5% CO2) at 37 0C. The culture medium was changed every 2 days until cells reached confluence and then were trypsinized with 0.25% (w/v) Trypsin - 0.53 mM EDTA (Sigma-Aldrich). 2.3.2 F-actin staining.

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  • MDCK.2

    Documentation. To download a certificate of analysis for MDCK.2 ( CRL-2936 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of MDCK.2 ( CRL-2936) is not currently available online. Complete this …

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  • Pre-set extrusion bioprinting for multiscale heterogeneous ...

    2018-6-6u2002·u2002The final concentration of cell-laden collagen was 3%. For pre-gelation of collagen, the bioink (e.g., cell-laden collagen) was printed onto DMEM with 10% fetal bovine serum (FBS) and 5% penicillin/streptomycin at 37 °C for 1 h, and then cut by surgical blade and moved to a …

    Get Price
  • Medical devices utilizing animal tissue

    Manufacturers of medical devices that use tissues of animal origin should be aware that, depending on the nature of the animal tissue or derivative, may need to consider regulation 722/2012. BSI recognizes that the requirements for medical device manufacturers who utilize tissues of …

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  • DMEM - Dulbecco's Modified Eagle Medium

    Gibco Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

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  • ClonaCell™-TCS Medium

    2015-3-6u2002·u2002ClonaCell™-TCS Medium is a serum-containing methylcellulose-based semi-solid medium that can be used for selecting and cloning a variety of suspension-adapted cell lines, including CHO-S and hybridomas. It can also be used for semi-solid cloning of some cell lines that grow adherently in the presence of serum, including CHO-K1, BHK-1, and HEK ...

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  • General catalog 7 th edition - FBS, Fetal Bovine Serum ...

    2020-2-27u2002·u2002Serum provides nearly all the components required for both adherent and suspension cell cultures, most of which are not yet chemically defined, such as growth factors, attachment factors, transporter proteins, lipids and hormones. Fetal Bovine Serum is the most widely used supplement for in vitro mammalian cell culture.

    Get Price
  • Fetal Bovine Serum

    Fetal Bovine Serum (FBS) is the most common serum used for this purpose; however, there are other serum types used in the culture of cells. Serum alternatives are also available to support cell growth if applicable for the cell type. VWR offers a wide variety of sera to meet the needs of cell culturists. Please select from the attributes below ...

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  • Fetal Bovine Serum

    2021-7-3u2002·u2002FETAL BOVINE SERUM Origin: United States triple 0.1 micron filtered - FBS-02-0050. Display Name : ... technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland ...

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  • INTERNATIONAL SERUM INDUSTRY ASSOCIATION

    2011-5-11u2002·u2002bovine serum, and these must be respected. Let us take the manipulation of fetal bovine serum and donor bovine serum, destined for use in the manufacture of a pharmaceutical product, as examples of processing and testing. The aseptically collected fetal or donor blood is refrigerated immediately after collection until coagulation is complete.

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  • Food safety considerations and research priorities for the ...

    Fetal bovine serum (FBS) is a common component of media, supplementing many components required for healthy cell growth (Post et al., 2020). However, due to ethical concerns related to the harvesting of FBS, along with high costs, batch-to-batch variability, and unpredictable availability, many cell-cultured meat and seafood developers are ...

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  • Medical devices utilizing animal tissue

    Manufacturers of medical devices that use tissues of animal origin should be aware that, depending on the nature of the animal tissue or derivative, may need to consider regulation 722/2012. BSI recognizes that the requirements for medical device manufacturers who utilize tissues of animal origin can be burdensome.

    Get Price
  • Testing of Chemicals - an overview

    1997-7-21u2002·u2002Indeed, fetal bovine serum, formulated media, and rudimentary cell lines were not available. In the 1950s, investigators developed media by defining the types of nutrients and their concentrations needed to achieve optimum growth of cells derived from animal and human tumors, though they recognized that undefined growth factors from fetal ...

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  • Assessing Toxicity and Nuclear and Mitochondrial DNA ...

    2018-4-12u2002·u2002Amplification reactions were performed with the mouse mitochondrial primers, for both long and short PCR, in a total volume of 50 μl containing 15 ng DNA template, KAPA Long Range Buffer (KAPA #3503), 100-ng/μl bovine serum albumin, 200 μM each of the four deoxynucleotide triphosphates, 400 nM each of the forward and reverse primers, 1.5-mM ...

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  • Antiproliferative and Antibacterial Activities of Cirsium ...

    Several Cirsium species are known for their uses in traditional medicine and consequently are studied for their phytochemical content and their biological activities. In the framework of a previous study conducted on eight extremophile plants from Tunisia, we highlighted that the crude methanolic extract of C. scabrum, a not investigated thistle, showed moderate but quite …

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  • Optimization of the isolation procedure and culturing ...

    2021-1-22u2002·u2002Abstract. Liver fibrosis (LF) mortality rate is approximately 2 million per year. Irrespective of the etiology of LF, a key element in its development is the transition of hepatic stellate cells (HSCs) from a quiescent phenotype to a myofibroblast-like cell with the production of fibrotic proteins. It is necessary to define optimal isolation and culturing conditions for good …

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  • Introduction to Fetal Bovine Serum Class - Biological ...

    Serum is commonly used as a supplement to basal growth medium in cell culture. The most common type of serum is fetal bovine serum (FBS) because of its high content of embryonic growth promoting factors. In cell culture, serum provides a wide variety of macromolecular proteins, low molecular weight nutrients, carrier proteins for water ...

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  • What BSA (Bovine Serum Albumin) Should I Use – Your BSA ...

    First, we should address what types of BSA exist in the market. GoldBio offers two types: protease free and fatty acid free ( I'll explain why fatty acid free is a good choice for many experiments later on in this article ). But then you have other types of BSA, such as standard BSA, low endotoxin, immunoglobulin free and blocking agent BSA.

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  • Multus Media: Crafting a sustainable future with ethically ...

    2020-11-12u2002·u2002The Need for Serum. With all of these mentioned challenges above, why do researchers and pharmaceutical companies still use it to grow cells? In short: fetal bovine serum supports the growth of a broad range of cell types from many relevant species and the cells tend to grow better in this serum than the alternatives that have been developed so far.

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  • Buy Cholesterol Concentrate from Bovine Se

    Bovine Cholesterol Concentrate is a concentrated cholesterol additive consisting mainly of bovine high density lipoproteins. The lipoproteins are substantially free of other serum proteins. Typical cholesterol concentrations are in the range of 6,000 to 8,000 mg/dL.

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  • Testing Services - VMRD

    2019-6-7u2002·u2002Bovine virus testing of bovine origin products EM-AOP-BOV Bovine (B): Serum EP 100ml/10g 22 days Comparative Titration/Serum Inhibitory Test EM-CT-AOP-BOV Bovine (B): Serum CPMP/BWP/1793/02 (2003) 5.3.4 100ml/10g 28 days Quantitative BVD assay test QUANT-AOP-BOV Bovine (B): Serum CVMP/CHMP/CPMP 100ml/10g 7 days

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  • Frontiers

    Salmonella Gallinarum only infects avian species, where it causes a severe systemic infection in birds of all ages. It is generally accepted that interaction with phagocytic cells plays an important role in the development of systemic, host-specific Salmonella infections. The current study detailed the interaction of S. Gallinarum with macrophages derived from chicken (HD11) and cattle …

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  • High-Efficiency Transduction of Primary Human ...

    2016-10-19u2002·u2002We have reported that of the 10 commonly used AAV serotype vectors, AAV6 is the most efficient in transducing primary human hematopoietic stem/progenitor cells (HSPCs). However, the transduction ...

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  • Equine Fetal, Adult, and Embryonic Stem Cell-Derived ...

    2019-10-23u2002·u2002Undifferentiated ESCs were cultured on mitotically inactivated mouse embryonic fibroblasts at 37°C, 5% CO 2 in ESC medium (DMEM/F12 containing 15% fetal bovine serum, 2 mM l-glutamine, 1% nonessential amino acids, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol) (all from Invitrogen), and 1,000 U/mL leukemia inhibitory factor (LIF) (Sigma ...

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  • Antiproliferative and Antibacterial Activities of Cirsium ...

    Several Cirsium species are known for their uses in traditional medicine and consequently are studied for their phytochemical content and their biological activities. In the framework of a previous study conducted on eight extremophile plants from Tunisia, we highlighted that the crude methanolic extract of C. scabrum, a not investigated thistle, showed moderate but quite …

    Get Price
  • 2019's 100 Best Companies To Work For In Utah - Zippia

    5. Autoliv. Zippia Score 4.8. Company Description: Autoliv Inc. is a Swedish automotive safety supplier with sales to all leading car manufacturers worldwide. Together with its joint ventures, Autoliv has over 67,000 employees in 27 countries, of whom 5700 are involved in research, development and engineering.

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