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  • Bovine Serum (Calf Serum)

    Bovine Serum (Calf Serum) Gibco® bovine (aka calf serum) serum is collected from prime cattle with an age range of 12-36 months, but typically less than 24 months. All lots are processed and manufactured in New Zealand. Each lot of bovine serum is tested for its ability to support the growth of VERO cells over three subcultures.

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  • Long-term growth comparison studies of FBS and FBS ...

    2017-6-7u2002·u2002Cells were detached with trypsin (0.05%)-EDTA or Accutase and cultured in medium containing fetal bovine serum (FBS), newborn calf serum (NBCS), and bovine calf serum (CS). Cultured cells were photographed at 18 and 36 h after seeding. (A) TW01, (B) OECM-1, and (C) FaDu cells. Black bar represents 50 μm. NBCS-G: Gibco NBCS; NBCS-H: HyClone NBCS.

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  • Cell Culture and Uses for Fetal Bovine Serum – Innovative ...

    Fetal bovine serum (FBS) is a product used in research laboratories, a blood product which exceeds cell culture testing standards and is used widely to promote growth medium. Because of its high quantity of hormones, carrier proteins and macromolecular proteins, fetal bovine serum is frequently present in culture medium used in in vitro fertilization.

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  • Fetal Bovine Serum - labome.com

    2021-10-17u2002·u2002Fetal bovine serum (FBS) is the liquid fraction of clotted blood from fetal calves, depleted of cells, fibrin and clotting factors, but containing a large number of nutritional and macromolecular factors essential for cell growth. Bovine serum albumin is the major component of FBS. Growth factors in FBS are essential for the maintenance and ...

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  • Introduction to Fetal Bovine Serum Collection

    Fetal bovine serum (FBS) is clotted whole fetal blood that has been centrifuged to remove still-present blood cells and used to supplement cell growth in culture media.. While other animal sera exist, FBS is the most common for research with cells due to its abundance of growth factors, which is ideal for propagating cell proliferation.

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  • Long-term growth comparison studies of FBS and FBS ...

    2017-6-7u2002·u2002Fetal bovine serum (FBS) is depended upon by investigators as an indispensable supplement in cell and tissue culture systems. Due to increased demand and limited availability, the price of FBS has increased by greater than 300% in the past few years. In addition, there are ethical and scientific controversies about the collection and use of FBS in culture systems.

    Get Price
  • Cell Culture Sera and Replacements - Creative Bioarray

    Cell Culture Sera and Replacements. Serum has been widely used as almost a universal growth additive in cell culture for so many years. Serum is a complex organic solution, which contains proteins, polypeptides, growth factors, adhesion and spreading factors, amino acids, vitamins, lipids, hormones, protease inhibitors and many other components.

    Get Price
  • FBS Basics

    Examples are Dialyzed, Charcoal Stripped, Ultra-low IgG, ES Cell, MSC and Exosome-Depleted. Newborn Calf Serum: Newborn Calf Serum (NBCS) is defined as the liquid fraction of clotted blood derived from healthy, slaughtered bovine calves aged less than 20 days, deemed fit for human consumption through ante- and/or post- mortem inspection.

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  • Vaccine Manufacturing - PubMed Central (PMC)

    2017-7-17u2002·u2002Cell culture are grown in roller bottles using fetal calf sera and M199 Hanks media for optimal cell growth. Chick embryo fibroblast cells are further infected by the viral working seed and incubated several days for viral culture. At the end of the viral culture, the cells are disrupted by mechanical lysis to release the virus.

    Get Price
  • Bovine Serum (Calf Serum)

    Bovine Serum (Calf Serum) Gibco® bovine (aka calf serum) serum is collected from prime cattle with an age range of 12-36 months, but typically less than 24 months. All lots are processed and manufactured in New Zealand. Each lot of bovine serum is tested for its ability to support the growth of VERO cells over three subcultures.

    Get Price
  • Long-term growth comparison studies of FBS and FBS ...

    2017-6-7u2002·u2002Cells were detached with trypsin (0.05%)-EDTA or Accutase and cultured in medium containing fetal bovine serum (FBS), newborn calf serum (NBCS), and bovine calf serum (CS). Cultured cells were photographed at 18 and 36 h after seeding. (A) TW01, (B) OECM-1, and (C) FaDu cells. Black bar represents 50 μm. NBCS-G: Gibco NBCS; NBCS-H: HyClone NBCS.

    Get Price
  • Cell Culture and Uses for Fetal Bovine Serum – Innovative ...

    Fetal bovine serum (FBS) is a product used in research laboratories, a blood product which exceeds cell culture testing standards and is used widely to promote growth medium. Because of its high quantity of hormones, carrier proteins and macromolecular proteins, fetal bovine serum is frequently present in culture medium used in in vitro fertilization.

    Get Price
  • Fetal Bovine Serum - labome.com

    2021-10-17u2002·u2002Fetal bovine serum (FBS) is the liquid fraction of clotted blood from fetal calves, depleted of cells, fibrin and clotting factors, but containing a large number of nutritional and macromolecular factors essential for cell growth. Bovine serum albumin is the major component of FBS. Growth factors in FBS are essential for the maintenance and ...

    Get Price
  • Introduction to Fetal Bovine Serum Collection

    Fetal bovine serum (FBS) is clotted whole fetal blood that has been centrifuged to remove still-present blood cells and used to supplement cell growth in culture media.. While other animal sera exist, FBS is the most common for research with cells due to its abundance of growth factors, which is ideal for propagating cell proliferation.

    Get Price
  • Long-term growth comparison studies of FBS and FBS ...

    2017-6-7u2002·u2002Fetal bovine serum (FBS) is depended upon by investigators as an indispensable supplement in cell and tissue culture systems. Due to increased demand and limited availability, the price of FBS has increased by greater than 300% in the past few years. In addition, there are ethical and scientific controversies about the collection and use of FBS in culture systems.

    Get Price
  • Cell Culture Sera and Replacements - Creative Bioarray

    Cell Culture Sera and Replacements. Serum has been widely used as almost a universal growth additive in cell culture for so many years. Serum is a complex organic solution, which contains proteins, polypeptides, growth factors, adhesion and spreading factors, amino acids, vitamins, lipids, hormones, protease inhibitors and many other components.

    Get Price
  • FBS Basics

    Examples are Dialyzed, Charcoal Stripped, Ultra-low IgG, ES Cell, MSC and Exosome-Depleted. Newborn Calf Serum: Newborn Calf Serum (NBCS) is defined as the liquid fraction of clotted blood derived from healthy, slaughtered bovine calves aged less than 20 days, deemed fit for human consumption through ante- and/or post- mortem inspection.

    Get Price
  • Vaccine Manufacturing - PubMed Central (PMC)

    2017-7-17u2002·u2002Cell culture are grown in roller bottles using fetal calf sera and M199 Hanks media for optimal cell growth. Chick embryo fibroblast cells are further infected by the viral working seed and incubated several days for viral culture. At the end of the viral culture, the cells are disrupted by mechanical lysis to release the virus.

    Get Price
  • Cell Culture Media for Vaccine Market Size, Trends, Shares ...

    2021-10-26u2002·u2002Cell culture media consists an appropriate source of energy and compounds, which supports growth of cells and regulate the cell cycle. A typical culture medium is composed of vitamins, inorganic salts, complement of amino acids, glucose, and serum which acts as source of growth factors, hormones, and attachment factors.

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  • Stress-induced signaling pathways in hyalin chondrocytes ...

    2017-1-12u2002·u2002tin-Fallavier, France) supplemented with 10% fetal calf serum (Invitrogen, Eragny, France), 100 U/ml penicillin, 0.1 mg/ml streptomycin and 4 mmol/l L-glutamine (Sigma) at 37 C in the presence of 5% CO 2. In each experiment, cells were made quiescent for 24 h in DMEM without serum and with 2% bo-vine albumin fatty acid free (Sigma).

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  • Basic Fibroblast Growth Factor Evokes a Rapid Glutamate ...

    2020-11-18u2002·u2002Worthington) at 37 °C. The dissociated cells were plated at a final density of 4 or 5 105/cm2 on polyethyleneimine-coated 12- and 24-well plates (4- and 2-cm2 surface area/well, respectively; Corning). The cul-ture medium consisted of 5% precolostrum newborn calf serum, 5% heated-inactivated horse serum, 1% rat serum, and 89% of a 1:1 mix-

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  • Cell Culture Media: A Review - labome.com

    2021-8-26u2002·u2002Cell culture media generally comprise an appropriate source of energy and compounds which regulate the cell cycle. A typical culture medium is composed of a complement of amino acids, vitamins, inorganic salts, glucose, and serum as a source of growth factors, hormones, and attachment factors. In addition to nutrients, the medium also helps ...

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  • Astroglial and Fibroblast Growth Factors Have Neurotrophic ...

    fetal calf serum (FCS) or the defined Ni additives (20) to which 0.25% bovine serum albumin (BSA) was added (HEBM/N1+BSA). Cell yields were as described previously (17-19). Preplating of ciliary ganglion cell suspensions of 2 x 105 cells in 2 ml of DMEM/10% FCS in 35-mm dishes for 2 hr enriched neurons to >95% purity. Eighty-five percent of

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  • Chemically defined medium for rat astroglial cells in ...

    1984-1-1u2002·u2002We have developed a serum-free defined medium that supports the growth in primary culture of rat astroglial cells. Cells dissociated from cerebral hem…

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  • Polymorphonuclear Leukocyte Adhesion Triggers the ...

    mented with 20% newborn calf serum, 50 ~g/ml endothelial cell growth supplement, and 100 p.g/ml heparin (M199 complete medium), and kept at 37°C in a 5% CO2 humidified atmosphere., as previously described (Lam- pugnani et al., 1995). The Joumal of Cell Biology, Volume 135, 1996 498

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  • A non-coding function of TYRP1 mRNA ... - Nature Cell Biology

    2017-10-9u2002·u2002MM lines were propagated in humidified air (37 °C, 5% CO 2) in flasks containing HAM-F10 medium supplemented with 5% heat-inactivated fetal bovine serum (FBS), 5% heat-inactivated newborn calf …

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  • AKT induces senescence in primary esophageal epithelial ...

    2006-10-9u2002·u2002Then cells were raised to a liquid–air interface and cultured in Epidermalization III medium for 6 days containing the same growth supplements as Epidermalization II, except for 2% newborn calf …

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  • Potential of Newborn and Adult Stem Cells for the ...

    Bypass surgeries using native vessels rely on the availability of autologous veins and arteries. An alternative to those vessels could be tissue-engineered vascular constructs made by self-organized tissue sheets. This paper intends to evaluate the potential use of mesenchymal stem cells (MSCs) isolated from two different sources: (1) bone marrow-derived MSCs and (2) umbilical cord blood ...

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  • Promotion of neurite outgrowth and protective effect of ...

    2007-9-9u2002·u2002Objective To clarify the effect of erythropoietin (EPO) on neurite outgrowth of the cultured retinal neurocytes, and investigate whether EPO might potentially be beneficial in protecting cultured retinal neurocytes suffering from glutamate-induced cytotoxity. Methods After the retinal neurocytes were cultured for 48 hours, the culture media was replaced with serum …

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  • Astroglial and Fibroblast Growth Factors Have Neurotrophic ...

    fetal calf serum (FCS) or the defined Ni additives (20) to which 0.25% bovine serum albumin (BSA) was added (HEBM/N1+BSA). Cell yields were as described previously (17-19). Preplating of ciliary ganglion cell suspensions of 2 x 105 cells in 2 ml of DMEM/10% FCS in 35-mm dishes for 2 hr enriched neurons to >95% purity. Eighty-five percent of

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  • A Functional Interaction between the Neuronal Adhesion ...

    1998-8-6u2002·u2002ImmunoResearch (Immunotech, Marseille, France). Cell cultures All of the cell culture media, fetal calf serum (FCS), and horse serum were purchased from Life Technologies (Gaithersburg, MD). Recombinant CHO cell lines. The CHO cell line LR-73 was maintained in DMEM supplemented with 10% FCS, 50 U/ml penicillin, and 50 mg/ml streptomycin.

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  • Food & Function - RSC Publishing Home

    vated newborn calf serum (Gibco), hEGF (10 µg L−1; PeproTech, London, UK), hbFGF (1 µg L−1; Gibco) and dexa-methasone (400 µg L−1) (Sigma), as described previously.32 Myocytes were differentiated upon confluency by switching to adifferentiation medium (5.5 mM glucose in DMEM, 2% horse serum (Gibco)) for up to 4 days.32 For the co ...

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  • Expression the establishment normal fibroblast

    arately encoding these two proteins could promote the estab- ... Saturation Growthability in Cell line* densityt agarosemedium, %* FR3T3 1.5 x 106 <0.002 PY-T21 >1.5 x 107 100 ... Growth properties in medium containing 10% newborn calf serumwere in all cases similar to those of established normal

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  • Address correspondence to

    2020-3-18u2002·u2002Peritoneal cells were harvested using 10 ml of DPBS to lavage the peritoneal cavity. Cells were resuspended in staining buffer (PBS containing 2% newborn calf serum) and pre-incubated with 0.5 µg/ml FcBlock (eBioscience) and stained with biotin-conjugated . Helix Pomatia. agglutinin (HPA: Sigma) and . mAbs and streptavidin-

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  • Ocular Fluid As a Replacement for Serum in Cell ...

    2015-7-2u2002·u2002Cryostorage is of immense interest in biomedical research, especially for stem cell-based therapies and fertility preservation. Several protocols have been developed for efficient cryopreservation of cells and tissues, and a combination of dimethyl sulfoxide (DMSO) and fetal bovine serum (FBS) is commonly used. However, there is a need for an alternative to FBS …

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  • Permissive Fatty Acid Incorporation Promotes ...

    2019-12-17u2002·u2002The fatty acid kinase FakA functions with FakB1 or FakB2, which orient specificity, for fatty acid incorporation in S. aureus (Parsons et al., 2014).The fakA and fakB1fakB2 mutants show attenuated virulence (Lopez et al., 2017), which could reflect the need for host fatty acid incorporation during infection.Although single fakB1 or fakB2 mutants may display partial …

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  • Promotion of neurite outgrowth and protective effect of ...

    2007-9-9u2002·u2002Objective To clarify the effect of erythropoietin (EPO) on neurite outgrowth of the cultured retinal neurocytes, and investigate whether EPO might potentially be beneficial in protecting cultured retinal neurocytes suffering from glutamate-induced cytotoxity. Methods After the retinal neurocytes were cultured for 48 hours, the culture media was replaced with serum …

    Get Price
  • Animals

    Animals. Animals is an international, peer-reviewed, open access journal devoted entirely to animals, including zoology and veterinary sciences, published monthly online by MDPI. Open Access — free for readers, with article processing charges (APC) paid by authors or their institutions.

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  • Short-Chain Glycoceramides Promote Intracellular ...

    2014-10-16u2002·u2002Purpose To improve therapeutic activity of mitoxantrone (MTO)-based chemotherapy by reducing toxicity through encapsulation in nanoliposomes and enhancing intracellular drug delivery using short-chain sphingolipid (SCS) mediated tumor cell membrane permeabilization. Methods Standard (MTOL) and nanoliposomes enriched with the SCS, C8-Glucosylceramide or …

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  • Effect of CPU-XT-008, a combretastatin A-4 analogue, on ...

    2015-11-5u2002·u2002The present study investigated the effect of the combretastatin A-4 analogue CPU-XT‑008 on the proliferation, apoptosis and expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF‑2) in human umbilical vein endothelial cells (HUVECs). The proliferation capacity of HUVECs was analyzed with a cell viability assay, while their apoptosis and migration ...

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  • Short-Chain Glycoceramides Promote Intracellular ...

    2014-10-16u2002·u2002Purpose To improve therapeutic activity of mitoxantrone (MTO)-based chemotherapy by reducing toxicity through encapsulation in nanoliposomes and enhancing intracellular drug delivery using short-chain sphingolipid (SCS) mediated tumor cell membrane permeabilization. Methods Standard (MTOL) and nanoliposomes enriched with the SCS, C8-Glucosylceramide or C8-Galactosylceramide …

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  • Activation of Flk-1/KDR mediates angiogenesis but not ...

    1997-11-1u2002·u2002(A) FBAE cells were seeded at 5000 per 12 multiwell plates in DMEM supplemented with 5% newborn calf serum, 2 mM glutamine and antibiotics. VEGF (2 ng/ml) or AIA or control immunoglobulins (PI-Ab, 8 ng/ml) were inoculated every other day and the cells trypsinized and counted after 4 …

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  • Open Access Journals

    We are an Open Access publisher and international conference Organizer. We own and operate 500 peer-reviewed clinical, medical, life sciences, engineering, and management journals and hosts 3000 scholarly conferences per year in the fields of clinical, medical, pharmaceutical, life sciences, business, engineering and technology.

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  • ASB2 targets filamins A and B to proteasomal degradation ...

    2008-12-15u2002·u2002NB4 and PLB985 cells were used as described. 2 HeLa and HEK293T cells were grown in Dulbecco modified Eagle medium (DMEM) containing 4.5 g/L glucose (Invitrogen, Carlsbad, CA) and 5% fetal bovine serum (PAA Laboratories, Coelbe, Germany). NIH3T3 cells were grown in DMEM containing 4.5 g/L glucose and 5% newborn calf serum (PAA Laboratories).

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  • Animals

    Animals. Animals is an international, peer-reviewed, open access journal devoted entirely to animals, including zoology and veterinary sciences, published monthly online by MDPI. Open Access — free for readers, with article processing charges (APC) paid by authors or their institutions.

    Get Price
  • Stimulating Effect of Bombesin on the Growth of ...

    2013-5-8u2002·u2002growth factor and glucocorticoids, are known to promote the growth of developing animals (5-7). Bombesin (BBS) is a tetradecapeptide isolated from amphibian skin. In mammals, BBS-like immu- noreactivity is present along the digestive tract. Bombesin has been shown to induce gastrin cell

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  • Comparing Astrocytic Cell Lines that Are Inhibitory or ...

    1999-10-15u2002·u2002Astrocytes, oligodendrocytes, and oligodendrocyte/type 2 astrocyte progenitors (O2A cells) can all produce molecules that inhibit axon regeneration. We have shown previously that inhibition of axon growth by astrocytes involves proteoglycans. To identify inhibitory mechanisms, we created astrocyte cell lines that are permissive or nonpermissive and showed that nonpermissive cells produce ...

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  • The Effect of Cinnamon Extract on Spermatogenesis and

    2016-12-23u2002·u2002of smooth muscle cell (SMC) (Philbrick . et al. 1996) and then could promote an adequate gastrointestinal function (Mok et al., 1989). According to Gao and Raj (2005), vasodilatation of SMC due to PTHrP appears to be mediated by cyclic adenosine monophosphate (cAMP) which activates a voltage-operated or calcium-dependent potassium channels.

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  • Cholesterol-regulated Translocation of NPC1L1 to the Cell ...

    Although NPC1L1 is required for intestinal cholesterol absorption, data demonstrating mechanisms by which this protein facilitates the process are few. In this study, a hepatoma cell line stably expressing human NPC1L1 was established, and cholesterol uptake was studied. A relationship between NPC1L1 intracellular trafficking and cholesterol uptake was apparent.

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  • An FGFR3/MYC positive feedback loop provides new ...

    They were cultured in DMEM supplemented with 10% newborn calf serum (NCS), 2 mM glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, and 400 μg/ml G418. For siRNA transfection, MGH-U3 and RT112 cells were used to seed six-well or 24-well plates at a density of 250,000 cells/well for MGH-U3 cells and 200,000 cells/well for RT112 cells.

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  • Cell Proliferation Kit (XTT based) - Biological Industries

    Cell Proliferation Kit (XTT) is an excellent solution for the quantitating of cell proliferation and viability, without using radioactive isotopes. It can be used as a non-radioactive substitute for cytotoxic tests based on the release of 51Cr from cells, maintaining high sensitivity.

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  • Thy1‐positive mesenchymal cells promote the maturation of ...

    2004-4-26u2002·u2002The dissociated cells were washed three times with PBS containing 3% fetal calf serum (FCS; ICN, Aurora, OH) and were used for fluorescence-activated cell sorter (FACS) analysis or FACS sorting. A flow diagram describing the formation of cell aggregates and FACS analysis and cell sorting is shown in Fig. 1.

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  • Medex Supply - Medical, Surgical, Durable Supplies and ...

    About MedexSupply.com . With one of the largest catalogs of medical, surgical, and diagnostic supplies available online, Medex Supply can provide your facility with all the medical equipment necessary to ensure a healthy, safe, and sterile environment.

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  • A New C-Xyloside Induces Modifications of GAG Expression ...

    2012-10-26u2002·u2002Proteoglycans (PGs) are critically involved in major cellular processes. Most PG activities are due to the large interactive properties of their glycosaminoglycan (GAG) polysaccharide chains, whose expression and fine structural features are tightly controlled by a complex and highly regulated biosynthesis machinery. Xylosides are known to bypass PG …

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  • Soft Tissue Repair with Easy-Accessible Autologous Newborn ...

    2017-12-17u2002·u2002Disrupted organogenesis leads to permanent malformations that may require surgical correction. Autologous tissue grafts may be needed in severe lack of orthotopic tissue but include donor site morbidity. The placenta is commonly discarded after birth and has a therapeutic potential. The aim of this study was to determine if the amnion from placenta or plasma rich of growth …

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  • Interkinetic nuclear movements promote apical expansion in ...

    2019-12-23u2002·u2002Author summary Pseudostratified epithelia (PSE) are a common type of epithelia characterized by the choreographed displacement of cells' nuclei along the apicobasal axis during proliferation. These so-called interkinetic movements (INM) were proposed to influence tissue expansion and suggested as culprit in several congenital diseases. INM rely on cytoskeleton …

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  • Technological, Regulatory, and Ethical Aspects of In Vitro ...

    2019-6-13u2002·u2002Defined as meat cultured in a laboratory within a bioreactor under controlled artificial conditions, in vitro meat is a relatively recent area that has opened a whole universe of possibilities and opportunities for the meat sector. With improved chemical and microbial safety and varied options, in vitro meat has been proposed as a green, healthy, environmentally friendly, and …

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  • Stimulating Effect of Bombesin on the Growth of ...

    2013-5-8u2002·u2002growth factor and glucocorticoids, are known to promote the growth of developing animals (5-7). Bombesin (BBS) is a tetradecapeptide isolated from amphibian skin. In mammals, BBS-like immu- noreactivity is present along the digestive tract. Bombesin has been shown to induce gastrin cell

    Get Price
  • Maternal/newborn Flashcards

    The client tells the nurse that her last menstrual cycle started on November 27th. Which of the following dates is the client's expected date of birth? ~September 3rd. ~September 20th. ~August 3rd. ~August 20th. September 3rd. A nurse is preparing to administer hepatitis B immune globulin to a newborn.

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