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  • versatile in Polyethylenimine

    Strasbourg,France), HeLa(A. Hall, MedicalResearchCoun-cil, London), and MRC-5 (Transgene, Strasbourg, France) cells were cultured in 75-cm2 flasks (Costar) in Dulbecco's modified Eagle's medium (DMEM, GIBCO) supplemented with 10% fetal calf serum (GIBCO), streptomycin at 100,ug/ml (GIBCO), penicillin at 100 international units/ml

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  • Factors associated with time to neonatal diarrhoea in ...

    2005-5-10u2002·u2002Calf stocking density (the number of calves per available area) was considered sufficient when a calf had at least 1.6 or 1 m 2 in the tie stall or free stall, respectively (Vallet et al., 1985). To assess passive immunity and the effectiveness of colostrum feeding, calves were sampled for total protein in the serum.

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  • Intermediate Conductance Ca2+-Activated K+ Channels ...

    2014-3-3u2002·u2002After each digestion, 100 ml of supernatant were obtained, layered onto 5 ml newborn calf serum and spun for 10 min at 2200 rpm (1000×g) at 20°C. Afterwards, pellets were resuspended in 1 ml Dulbecco's modified Earle's medium (DMEM; Invitrogen, Paisley, UK) and centrifuged for 10 min at 2200 rpm.

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  • Isolation, preliminary characterize Ltion, and interferon ...

    newborn calf serum, glutamine (2 mM), and antibiotics. For some experimental procedures, the serum concentration was decreased to 2%. Medium prepared without tryptose, glucose, or serum but with antibiotics was used for cell agglutination tests. Viruses. Encephalomyocarditis virus (EMC) and …

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  • Calf health from birth to weaning. I. General aspects of ...

    2011-9-16u2002·u2002Calfhood diseases have a major impact on the economic viability of cattle operations. This is the first in a three part review series on calf health from birth to weaning, focusing on preventive measures. The review considers both pre- and periparturient management factors influencing calf health, colostrum management in beef and dairy calves and further nutrition and …

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  • Open Access Journals

    We are an Open Access publisher and international conference Organizer. We own and operate 500 peer-reviewed clinical, medical, life sciences, engineering, and management journals and hosts 3000 scholarly conferences per year in the fields of clinical, medical, pharmaceutical, life sciences, business, engineering and technology.

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  • Home - DeLaval

    Emerging Stronger Together. Maintaining a 135-year legacy of innovations for dairy farmers doesn't come without its challenges – including pandemics and crises. Throughout two world wars and several pandemics and outbreaks, DeLaval has adapted to continue providing world-class solutions to dairy farmers around the globe.

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  • Anticancer Effect of Plant-Derived Polysaccharides on Mice

    2013-12-24u2002·u2002pelling wind, removing dampness, diminishing inflam- mation, relieving pain and other effects, it is widely used to treat diphtheria, sore throat, stomach pain, bruises, rheumatism, carbuncle swollen sore, snake bites and ra- bies, etc. Previous studies found that Tupistra chinensis has anti-inflammatory and other pharmacological activi-

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  • The Trusted Provider of Medical Information since 1899

    The Trusted Provider of Medical Information since 1899. The Merck Manuals are a comprehensive medical information source covering thousands of topics in all fields of medicine. They are offered as a free public service to health care professionals and the general public. Share.

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  • Calf health from birth to weaning. I. General aspects of ...

    2011-9-16u2002·u2002Calfhood diseases have a major impact on the economic viability of cattle operations. This is the first in a three part review series on calf health from birth to weaning, focusing on preventive measures. The review considers both pre- and periparturient management factors influencing calf health, colostrum management in beef and dairy calves and further nutrition and weaning in dairy calves.

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  • Factors associated with time to neonatal diarrhoea in ...

    2005-5-10u2002·u20021.. IntroductionWe studied diarrhoea in newborn calves (the major cause of neonatal mortality) Sivula et al., 1996a, Wells et al., 1996, Bendali et al., 1999b.Calves are at the greatest risk of diarrhoea during the first week of life, and this risk decreases with age Vallet et al., 1985, Waltner-Toews et al., 1986.The incidence risks of diarrhoea in calves <30 days old reported by several ...

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  • Humoral immune response to repeated lumpy skin disease ...

    2019-3-6u2002·u2002VNT is the only OIE-validated test for detection of LSDV specific antibody. For VNT and IFAT, MDBK cells were grown using DMEM supplemented with 10% foetal calf serum (FCS) and incubated at 37 °C with 5% CO2. VNT. All tested serum samples including positive and negative control serum were incubated at 56 °C for 30 min.

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  • Open Access Journals

    We are an Open Access publisher and international conference Organizer. We own and operate 500 peer-reviewed clinical, medical, life sciences, engineering, and management journals and hosts 3000 scholarly conferences per year in the fields of clinical, medical, pharmaceutical, life sciences, business, engineering and technology.

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  • Magnesium therapy improves outcome in

    2017-12-17u2002·u2002For induction of PLY-based animal brain swelling, 29 infant SD rats (P10–14)wereused.Thechoiceofthemodelwas based on our earlier experience that showed more efficient i.c.v. toxin distribution in the ventricular system of rats, than in mice, when toxin-induced swelling was studied (Hupp et al., 2012). Rats were randomly distributed to form

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  • Scope Heal - Be at Ease without Disease

    Latest news on Health, Nutrition, Physical Exercise, Psychology and Well-being from experts in the sector. With ScopeHeal you will learn to feel good. Do not miss it!

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  • (PDF) Jin15 Angewandte Chemie SI - ResearchGate

    115 µ L with D.I. water. Then 5 µ L of freshly prepared 1M tris (2-carboxyethyl) phosphine. (TCEP, non sulfur based reducing agent) was spiked into the solution and mixed by brief. vortexing ...

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  • (PDF) Proc. Natl. Acad. Sci

    PDF On Jul 1, 1995, O. Boussif and others published Proc. Natl. Acad. Sci Find, read and cite all the research you need on ResearchGate

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  • ZARA Official Website

    Albania Algérie Andorra Armenia Argentina Aruba Australia Azerbaijan Bahrain Belgium Беларусь/Belarus Bosnia And Herzegovina Brasil България Bulgaria Canada Chile MAINLAND CHINA 中国大陆 Hong Kong SAR 香港特別行政區 Macau SAR 澳門特別行政區 Taiwan, China 中國台灣 Colombia Costa Rica Cyprus Česká republika Danmark Deutschland Germany Ecuador ...

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  • Study 136 Terms

    5. Pain in calf muscles 6. Warmth and redness over vein 7. Swelling in calf or leg 8. Cyanosis or pallor extremity 9. Skin that is cool to touch 10. Edema 11. Thin shiny skin w …

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  • versatile in Polyethylenimine

    Strasbourg,France), HeLa(A. Hall, MedicalResearchCoun-cil, London), and MRC-5 (Transgene, Strasbourg, France) cells were cultured in 75-cm2 flasks (Costar) in Dulbecco's modified Eagle's medium (DMEM, GIBCO) supplemented with 10% fetal calf serum (GIBCO), streptomycin at 100,ug/ml (GIBCO), penicillin at 100 international units/ml

    Get Price
  • Isolation, preliminary characterize Ltion, and interferon ...

    newborn calf serum, glutamine (2 mM), and antibiotics. For some experimental procedures, the serum concentration was decreased to 2%. Medium prepared without tryptose, glucose, or serum but with antibiotics was used for cell agglutination tests. Viruses. Encephalomyocarditis virus (EMC) and vesicular

    Get Price
  • Intermediate Conductance Ca2+-Activated K+ Channels ...

    2014-3-3u2002·u2002After each digestion, 100 ml of supernatant were obtained, layered onto 5 ml newborn calf serum and spun for 10 min at 2200 rpm (1000×g) at 20°C. Afterwards, pellets were resuspended in 1 ml Dulbecco's modified Earle's medium (DMEM; Invitrogen, Paisley, UK) and centrifuged for …

    Get Price
  • Factors associated with time to neonatal diarrhoea in ...

    2005-5-10u2002·u2002Calf stocking density (the number of calves per available area) was considered sufficient when a calf had at least 1.6 or 1 m 2 in the tie stall or free stall, respectively (Vallet et al., 1985). To assess passive immunity and the effectiveness of colostrum feeding, calves were sampled for total protein in the serum.

    Get Price
  • Calf health from birth to weaning. I. General aspects of ...

    2011-9-16u2002·u2002Calfhood diseases have a major impact on the economic viability of cattle operations. This is the first in a three part review series on calf health from birth to weaning, focusing on preventive measures. The review considers both pre- and periparturient management factors influencing calf health, colostrum management in beef and dairy calves and further nutrition and weaning in dairy calves.

    Get Price
  • Anticancer Effect of Plant-Derived Polysaccharides on Mice

    2013-12-24u2002·u2002pelling wind, removing dampness, diminishing inflam- mation, relieving pain and other effects, it is widely used to treat diphtheria, sore throat, stomach pain, bruises, rheumatism, carbuncle swollen sore, snake bites and ra- bies, etc. Previous studies found that Tupistra chinensis has anti-inflammatory and other pharmacological activi-

    Get Price
  • Home - DeLaval

    Emerging Stronger Together. Maintaining a 135-year legacy of innovations for dairy farmers doesn't come without its challenges – including pandemics and crises. Throughout two world wars and several pandemics and outbreaks, DeLaval has adapted to continue providing world-class solutions to dairy farmers around the globe.

    Get Price
  • Open Access Journals

    We are an Open Access publisher and international conference Organizer. We own and operate 500 peer-reviewed clinical, medical, life sciences, engineering, and management journals and hosts 3000 scholarly conferences per year in the fields of clinical, medical, pharmaceutical, life sciences, business, engineering and technology.

    Get Price
  • The Trusted Provider of Medical Information since 1899

    The Trusted Provider of Medical Information since 1899. The Merck Manuals are a comprehensive medical information source covering thousands of topics in all fields of medicine. They are offered as a free public service to health care professionals and the general public. Share.

    Get Price
  • intranet.emmc.org

    intranet.emmc.org

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  • Maternal ω3 docosapentaenoic acid inhibits infant allergic ...

    2020-2-6u2002·u2002The dorsal halves of the ears were incubated for 30 min at 37°C in 0.5 M ammonium thiocyanate. Subsequently, the dermal sheets were separated and fixed in acetone for 10 min at –20°C. To block nonspecific binding, the sheets were incubated for 30 min in PBS containing 2% newborn calf serum (NCS) at room temperature.

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  • Evaluation of in vitro culture systems for the maintenance ...

    2018-5-2u2002·u2002Evaluation of the effect of each basic culture medium on the viability of L. loa L3 and mf. The T 90 values of the parasite motility in different basic media were evaluated and results are presented in Fig. 1 and the statistical report in Table 1.The values of T 90 ranged from 3.0 day to 6.5 days for L3. In the absence of protein supplement and feeder cells, there were significant difference ...

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  • Rotavirus in Calves and Its Zoonotic Importance

    Rotavirus is a major pathogen responsible for diarrheal disease in calves, resulting in loss of productivity and economy of farmers. However, various facets of diarrheal disease caused by rotavirus in calves in the world are inadequately understood, considering that diarrheal disease caused by rotavirus is a vital health problem in calves that interrupts production benefits with reduced weight ...

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  • Mutations in the cofilin partner Aip1/Wdr1 cause ...

    2007-10-1u2002·u2002Clonal cultures of hematopoietic cells were performed as described. 40 Cultures of 2.5 × 10 4 adult bone marrow cells or 5 × 10 4 spleen cells in 1 mL of 0.3% agar in Dulbecco modified Eagle medium (DMEM) supplemented with newborn calf serum (20%) were stimulated with 100 ng/mL murine stem cell factor (SCF), 10 ng/mL murine IL-3, and 4 U/mL ...

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  • An overview of calf diarrhea - infectious etiology ...

    Calf diarrhea (also known as calf scouring) is a commonly reported disease and a major cause of economic loss to cattle producers. The 2007 National Animal Health Monitoring System (NAHMS) for U.S. dairy [] reported that 57% of weaning calf mortality was due to diarrhea and most cases occurred in calves less than 1 month old.A similar mortality rate (53.4%) for dairy calves due to calf ...

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  • Frontiers

    2020-11-23u2002·u2002Mycobacterial diseases of cattle are responsible for considerable production losses worldwide. In addition to their importance in animals, these infections offer a nuanced approach to understanding persistent mycobacterial infection in native host species. Mycobacteriumavium ssp. paratuberculosis (MAP) is an enteric pathogen that establishes a persistent, asymptomatic infection in …

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  • Neuroprotective Effect of TREM-2 in Aging and

    After centrifugation for 3 min at 1000 g, cells were re-suspended in culture medium [in DME, 10% fetal calf serum, Sera Lab, UK; 1% PSF, Gibco] and plated in 75 cm 3 poly-l-lysine-coated tissue culture flasks (Nunclon, Life Technologies, Paisley, Scotland) at a density of 2 cortices per flask. To prepare the microglial culture, after the cells ...

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  • Boots Advanced Adjustable Ankle Support - Boots

    Boots has products available in other retail outlets in a number of countries, select from the country-specific sites below to find out more: Bahrain Hong Kong Kuwait Qatar. Saudi Arabia Singapore Thailand United Arab Emirates. Boots Health Hub. Online access …

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    Kids' Halloween Skeleton Matching Family Pajama Set - Black. $7.99. Hero Cosmetics Mighty Patch Original Acne Pimple Patches - 24ct. $99.99. Apple AirTag (4 Pack) $30.00 - $329.00. Vintage Distressed Rug - Threshold™. $7.99. Baby 3pk Long Sleeve Bodysuit - Just One You® made by carter's White.

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  • Cell-volume-dependent vascular smooth muscle

    2004-8-4u2002·u2002Cell volume modulation in hyperosmotically (a, b) and isosmotically (c) shrunken vascular smooth muscle rings (VSMC). a Kinetics of cell volume modulation in hyperosmotic medium.WKY-7 cells were incubated for time intervals indicated on the x-axis in 0.5 ml physiologically balanced salt solution (PSS) containing 2 μCi/ml [14 C]-urea. At the time point indicated by the arrow, 0.5 ml of PSS (1 ...

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  • Controlling the kinetic chain length of the crosslinks in ...

    2013-1-31u2002·u2002Samples were then extracted with serum free culture medium (DMEM), 1 ml per 3 cm 2 surface area) at 37 °C for 24 h. The supernatants were stored at −20 °C until further use. NIH 3T3 fibroblasts were cultured in T75 cell culture flasks in DMEM medium supplemented with 10 % newborn calf serum and 1 % penicillin/streptomycin.

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  • Attenuation of RNA viruses by redirecting their evolution ...

    2017-6-5u2002·u2002HeLa cells were maintained in DMEM medium with 10% new born calf serum (NBCS), and MDCK cells were maintained in MEM medium with 5% fetal calf serum (FCS). ... then resuspended in 1 ml swelling ...

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  • Edema: Diagnosis and Management - AAFP Home

    2013-7-15u2002·u2002Acute swelling of a limb over a period of less than 72 hours is ... distribution in later stages Soft, pitting edema with reddish-hued skin; predilection for …

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  • NCLX prevents cell death during adrenergic activation of ...

    2020-7-3u2002·u2002Finally, cell pellets (preadipocytes) were re-suspended 5 ml growth medium (DMEM supplemented with 20% newborn calf serum (NCS), 4 mM glutamine, 10 mM HEPES, 0.1 mg/ml sodium ascorbate, 50 U/ml ...

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  • Differential Diagnosis, Investigation, and Current ...

    The Leg-O-Meter (François Zuccarelli, MD, Hôpital St-Michel, Service de Chirurgie Vasculaire, Départment de Phlébologie et d'Angeiologie, Paris, France) is designed to measure the circumference of the ankle or calf. 43 This has high interobserver reliability and is easy to use. It has been mainly used in assessing leg swelling related to ...

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  • Rotavirus in Calves and Its Zoonotic Importance

    Rotavirus is a major pathogen responsible for diarrheal disease in calves, resulting in loss of productivity and economy of farmers. However, various facets of diarrheal disease caused by rotavirus in calves in the world are inadequately understood, considering that diarrheal disease caused by rotavirus is a vital health problem in calves that interrupts production benefits with …

    Get Price
  • In vitro and in vivo activities of DW-3-15, a commercial ...

    2019-5-3u2002·u2002The effects of commercial DW-3-15 on the viability and oviposition of adult and juvenile S. japonicum worms were assessed in vitro.Moreover, the antiparasitic activity of commercial DW-3-15 in a mouse model of S. japonicum infection was investigated, using PZQ as a positive control. Worm burden, egg burden and diameter of granuloma in the liver were evaluated in all the …

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  • Swollen Face on One Side

    2020-8-27u2002·u2002Swelling on one side of the face can present itself in different parts of the face like a swollen cheek or eye. Common causes of swelling could be allergy symptoms on one side of the face, a facial injury, or a skin infection. Read below for more information on associated symptoms, other causes, and treatment options on how to reduce facial swelling on one side.

    Get Price
  • OJHAS: The Online Journal of Health and Allied Sciences

    2018-1-1u2002·u2002Title. A Survey Report of Non-Communicable Diseases in Village Khubi of Karad Block Maharashtra, India: A Retrospective Observational Study. Status. Submitted: Aug 1, 2018 Not accepted Oct 14, 2018. # 1997. Title. Integrated Approach in the management of acid reflux, hypertension and obesity.

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  • Preservation of Preloaded DMEK Lenticules in Dextran and ...

    2016-7-2u2002·u2002Purpose. To determine the optimum preservation conditions for preloading DMEK lenticules using organ culture system. Methods. 8.5 mm DMEK lenticules were stripped and preserved with endothelium flap-in for 4 days at RT in an IOL cartridge that was blocked with rubber stoppers from each end. In C1, tissues were collected from tissue culture medium (TCM) and …

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  • Attenuation of RNA viruses by redirecting their evolution ...

    2017-6-5u2002·u2002HeLa cells were maintained in DMEM medium with 10% new born calf serum (NBCS), and MDCK cells were maintained in MEM medium with 5% fetal …

    Get Price
  • Edema: Diagnosis and Management - AAFP Home

    2013-7-15u2002·u2002Edema Table 2. Diagnosis and Management of Common Causes of Localized Edema. Etiology Onset and location Examination findings Evaluation methods Treatment

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  • Viral Polymerase-Helicase Complexes Regulate Replication ...

    Cells and viruses. The mammalian Vero, HeLa, and BHK-21 cell lines were maintained in Dulbecco modified Eagle medium (DMEM; Gibco) supplemented with 10% newborn calf serum (Gibco) and 1% penicillin-streptomycin (Sigma) at 37°C with 5% CO 2.. Wild-type (WT) CHIKV was generated from the La Reunion strain 06-049 infectious clone, as previously described ().

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  • NCLX prevents cell death during adrenergic activation of ...

    2020-7-3u2002·u2002Finally, cell pellets (preadipocytes) were re-suspended 5 ml growth medium (DMEM supplemented with 20% newborn calf serum (NCS), 4 mM glutamine, 10 mM HEPES, 0.1 mg/ml sodium ascorbate, 50 U/ml ...

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  • Frusemix-M Tablets Frusemix Tablets - NPS MedicineWise

    2021-11-4u2002·u2002The name of your medicine is FRUSEMIX-M (20 mg tablet) FRUSEMIX (40 mg tablets). It contains the active ingredient furosemide (frusemide). Furosemide (frusemide) belongs to a family of drugs called diuretics. A diuretic helps reduce the amount of excess fluid in the body by increasing the amount of urine produced.

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  • Preservation of Preloaded DMEK Lenticules in Dextran and ...

    2016-7-2u2002·u2002Purpose. To determine the optimum preservation conditions for preloading DMEK lenticules using organ culture system. Methods. 8.5 mm DMEK lenticules were stripped and preserved with endothelium flap-in for 4 days at RT in an IOL cartridge that was blocked with rubber stoppers from each end. In C1, tissues were collected from tissue culture medium (TCM) and preserved in TCM.

    Get Price
  • In vitro and in vivo activities of DW-3-15, a commercial ...

    2019-5-3u2002·u2002The effects of commercial DW-3-15 on the viability and oviposition of adult and juvenile S. japonicum worms were assessed in vitro.Moreover, the antiparasitic activity of commercial DW-3-15 in a mouse model of S. japonicum infection was investigated, using PZQ as a positive control. Worm burden, egg burden and diameter of granuloma in the liver were evaluated in all the treatment groups.

    Get Price
  • Swollen Face on One Side

    2020-8-27u2002·u2002Swelling on one side of the face can present itself in different parts of the face like a swollen cheek or eye. Common causes of swelling could be allergy symptoms on one side of the face, a facial injury, or a skin infection. Read below for more information on associated symptoms, other causes, and treatment options on how to reduce facial swelling on one side.

    Get Price
  • OJHAS: The Online Journal of Health and Allied Sciences

    2018-1-1u2002·u2002Title. A Survey Report of Non-Communicable Diseases in Village Khubi of Karad Block Maharashtra, India: A Retrospective Observational Study. Status. Submitted: Aug 1, 2018 Not accepted Oct 14, 2018. # 1997. Title. Integrated Approach in the management of …

    Get Price
  • Step To Health - This website publishes free articles on ...

    This website publishes free articles on natural remedies, physical and mental health, and how to take care of your body.

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