Intermediate Conductance Ca2+-Activated K+ Channels ...
2014-3-3u2002·u2002After each digestion, 100 ml of supernatant were obtained, layered onto 5 ml newborn calf serum and spun for 10 min at 2200 rpm (1000×g) at 20°C. Afterwards, pellets were resuspended in 1 ml Dulbecco's modified Earle's medium (DMEM; Invitrogen, Paisley, UK) and centrifuged for 10 min at 2200 rpm.
Get Price