Rapid and reliable hybridoma screening method that is ...
2021-6-1u2002·u2002Hybridomas were subjected to antigen-mediated fluorescent labeling. His-EGFP recombinant protein (2 nmol) was mixed with hybridoma in 1 mL RPMI 1640 medium supplemented with 10% Newborn Calf Serum (Biowest, Nuaillé, France), and the cells were incubated for 2 h at 37 °C in 5% CO 2. The cells were centrifuged in RPMI 1640 medium at 800 rpm for ...
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