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  • Up-Regulation of Superoxide Dismutase 2 in 3D Spheroid ...

    Superoxide dismutase activity was measured using the superoxide dismutase assay kit, according to the manufacturer's instructions (Sigma). SOD2 activity was measured in the presence of a copper/zinc superoxide dismutase inhibitor (Cu/Zn SOD). Data were normalized to protein content. Absorbance values were measured at 450 nm.

    Get Price
  • Assay of superoxide dismutase activity in a plate assay ...

    2016-12-1u2002·u2002One of the most convenient methods for measuring superoxide dismutase activity is a plate assay using xanthine oxidase and the water soluble tetrazolium WST-1.

    Get Price
  • Conjugates of Superoxide Dismutase 1 with Amphiphilic

    The enzyme superoxide dismutase 1 (SOD1, also known as Cu,Zn/SOD) efficiently catalyzes the dismutation of superoxide into oxygen and hydrogen peroxide. It is a stable homodimer of identical 16 kDa subunits held together primarily by hydrophobic interactions and cofactored with copper and zinc 8, …

    Get Price
  • Autophagy and mitochondrial dysfunction in adjuvant ...

    2016-9-9u2002·u2002Superoxide anion is regulated by enzymes such as superoxide dismutase (SOD) and peroxidases, as well as endogenous supplies of antioxidants such as glutathione (GSH) 40. Resveratrol is a phenolic compound, which is widely found in various fruits and vegetables, possess antioxidant, anti-aging, regulation of lipid metabolism, anti-cancer ...

    Get Price
  • Changes of stress oxidative enzymes in rat mammary

    Blood samples u200ewere u200ecentrifuged at 2,000 g for 10 min at 4 ˚C u200eu200f)u200fModel 5804 centrifuge; Eppendorf AG, Hamburg, Germanyu200e)u200e to obtain serum and stored in – 20 ˚C until enzyme analysis.u200e The procedures were u200eperformed according to u200ethe u200emanufacturer's protocol like u200ethe mammary tissues supernatant.

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  • Tumor suppressive activity of a variant isoform of ...

    This led to the previously reported observation that a liposarcoma‐derived cell line (LSA), released (a) factor(s) able to kill some tumor‐derived mammary epithelial cells, while sparing the corresponding normal cells. 11, 12, 13 In the present investigation, a variant form of human manganese superoxide dismutase (MnSOD) was identified as a ...

    Get Price
  • Protective Effect of Melatonin and Catalase in Bovine ...

    Superoxide production by bovine neutrophils. Neutrophils (1 × 10 6 /ml) were incubated in presence or absence of lipopolysaccharide (LPS) (1 μ g/ml) for 48 hrs at 37 ° C and 5% CO 2 .

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  • Autophagy and mitochondrial dysfunction in adjuvant ...

    2016-9-9u2002·u2002Resveratrol is a polyphenol derivatives which exhibits a pro-apoptotic effect in a variety of human cancers by triggering mitochondria apoptosis pathway and autophagy. However, there are …

    Get Price
  • The protective effects of Ribes diacanthum Pall on ...

    2016-2-3u2002·u2002Ethnopharmacological relevance. Ribes diacanthum Pall. (Saxifragaceae), a Mongolian folk medicinal plant, was used to treat urinary system diseases. The present work aims to investigate the protective effects of Ribes diacanthum Pall (RDP) against cisplatin-induced nephrotoxicity.

    Get Price
  • NF-κB-Gasdermin D (GSDMD) Axis Couples Oxidative Stress ...

    2018-8-30u2002·u2002Superoxide dismutase (SOD) activity. SOD activity was evaluated using a kit according to the manufacturer's instructions. Cells (1×10 7) were homogenized in 10 mM phosphate buffer solution (1 mL; pH 7.4).

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  • Swimming attenuates inflammation, oxidative stress, and ...

    2017-1-31u2002·u2002Swimming attenuates DSS-induced spleen enlargement, reduction of BW gain, and colon injury. We initially found that oral administration of DSS caused significant decrease in BW gain (Figure (Figure1A), 1A), spleen swelling (Figures (Figures1B 1B and and1C), 1C), elevated serum LDH (Figure (Figure1D), 1D), colon shortening (Figure (Figure1E), 1E), and colonic barrier …

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  • Acute ethanol exposure increases the susceptibility of the ...

    2012-11-14u2002·u2002Densitometric analysis of bands for (A) superoxide dismutase (SOD)-1 showed a significant increase after 6 h and 24 h ethanol administration, and (B) glutathione peroxidase (GPx)-4, (C) cytochrome-c oxidase (cyto-c oxi) and (D) MMP-9 following 24 h, compared with the control group. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

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  • Superoxide dismutase 1 and glutathione peroxidase 1 are ...

    2016-1-1u2002·u2002Total RNA was extracted from harvested cells using the Chomczynski extraction method (Chomczynski and Sacchi, 1987).The quality and integrity of the RNA were assessed by gel electrophoresis using a 1.0% agarose, and the purity was measured based on the 260/280 nm and 260/230 nm ratios.The RNA (1 μl) was reverse-transcribed with oligo dT primers using the one …

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  • Assay of superoxide dismutase activity in a plate assay ...

    2016-12-1u2002·u2002One of the most convenient methods for measuring superoxide dismutase activity is a plate assay using xanthine oxidase and the water soluble tetrazolium WST-1.

    Get Price
  • Tumor suppressive activity of a variant isoform of ...

    This led to the previously reported observation that a liposarcoma‐derived cell line (LSA), released (a) factor(s) able to kill some tumor‐derived mammary epithelial cells, while sparing the corresponding normal cells. 11, 12, 13 In the present investigation, a variant form of human manganese superoxide dismutase (MnSOD) was identified as a ...

    Get Price
  • (PDF) Minute quantities of misfolded mutant superoxide ...

    Mutant forms of superoxide dismutase-1 (SOD1) cause amyotrophic lateral sclerosis (ALS) by an unknown noxious mechanism. Using an antibody against a novel epitope in …

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  • Protective Effect of Melatonin and Catalase in Bovine ...

    Superoxide production by bovine neutrophils. Neutrophils (1 × 10 6 /ml) were incubated in presence or absence of lipopolysaccharide (LPS) (1 μ g/ml) for 48 hrs at 37 ° C and 5% CO 2 .

    Get Price
  • (PDF) ASSESSMENT OF IMMUNE PROTECTIVE CAPACITY OF

    2011-12-30u2002·u2002assessment of immune protective capacity of the recombinant iron superoxide dismutase (fesod) from bordetella pertussis December 2011 DOI: 10.13140/2.1.4746.0808

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  • The protective effects of Ribes diacanthum Pall on ...

    2016-2-3u2002·u2002Ethnopharmacological relevance. Ribes diacanthum Pall. (Saxifragaceae), a Mongolian folk medicinal plant, was used to treat urinary system diseases. The present work aims to investigate the protective effects of Ribes diacanthum Pall …

    Get Price
  • NF-κB-Gasdermin D (GSDMD) Axis Couples Oxidative

    2018-8-30u2002·u2002Superoxide dismutase (SOD) activity. SOD activity was evaluated using a kit according to the manufacturer's instructions. Cells (1×10 7) were homogenized in 10 mM phosphate buffer solution (1 …

    Get Price
  • (PDF) ASSESSMENT OF IMMUNE PROTECTIVE CAPACITY

    2011-12-30u2002·u2002assessment of immune protective capacity of the recombinant iron superoxide dismutase (fesod) from bordetella pertussis December 2011 DOI: 10.13140/2.1.4746.0808

    Get Price
  • Ceria-based nanotheranostic agent for rheumatoid arthritis

    2020-10-25u2002·u2002Treatment of rheumatoid arthritis by targeting macrophages through folic acid tailored superoxide dismutase and serratiopeptidase. J Drug Deliv Sci Technol. 2017;41:431-435 50. Younus H. Therapeutic potentials of superoxide dismutase. Int J Health Sci. 2018;12:88-93 51. Tan H-Y, Wang N, Li S, Hong M, Wang X, Feng Y.

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  • Jiuzhuan Huangjing Pills relieve mitochondrial dysfunction ...

    2021-10-1u2002·u2002Background. Metabolic dysfunction-associated fatty liver disease (MAFLD) is a common global chronic liver disease. Jiuzhuan Huangjing Pills (JHP) have been used for the treatment of human disease for over a thousand years, but their efficacy and underlying mechanism(s) of action against MAFLD are unknown.

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  • Regulation of Heat Shock Proteins 27 and 70, p-Akt/p

    2013-12-6u2002·u2002After completion of the reperfusion period, blood samples were withdrawn from the heart and serum was separated via centrifugation (Heraeus Biofuge, Germany) at 3000g for 5 min. Thereafter, animals were sacrificed under an overdose of anesthesia (pentobarbitone sodium 100 mg/kg, i.v.), and their hearts were excised and processed for ...

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  • (PDF) Minute quantities of misfolded mutant superoxide ...

    Mutant forms of superoxide dismutase-1 (SOD1) cause amyotrophic lateral sclerosis (ALS) by an unknown noxious mechanism. Using an antibody against …

    Get Price
  • Glycoursodeoxycholic Acid Reduces Matrix

    2014-5-22u2002·u2002Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that affects mainly motor neurons (MNs). NSC-34 MN-like cells carrying the G93A mutation in human superoxide dismutase-1 (hSOD1G93A) are a common model to study the molecular mechanisms of neurodegeneration in ALS. Although the underlying pathways of MN failure still remain elusive, increased apoptosis and oxidative ...

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  • Protective Effect of Crocodile Hemoglobin and Whole

    2016-11-12u2002·u2002A putative protective effect of cHb and cWb against H2O2-induced oxidative damage was evaluated in detail using MRC-5 cells. In addition, the carrageenan (Carr)-induced mouse paw edema model and the cotton pellet-induced granuloma model were employed to examine the in vivo anti-inflammatory activity of cHb and cWb in mice. It was demonstrated that both cHb and cWb treatments …

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  • Beneficial effect of 20‑hydroxyecdysone exerted by ...

    2017-8-29u2002·u2002Rheumatoid arthritis is a chronic autoimmune disease characterized by an elevated synovial inflammatory response, with destruction or erosion of articular cartilage in major joints. The aim of the present study was to examine whether 20‑hydroxyecdysone (HES) is able to ameliorate oxidative stress and inflammatory responses in a collagen‑induced rheumatoid arthritis (CIA) rat model.

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  • Antioxidants

    As a well-known hepatoprotective and antioxidant agent, dimethyl diphenyl bicarboxylate (DDB) has frequently been employed to remedy various liver diseases. However, it is still uncertain whether DDB exerts consistent hepatoprotective and antioxidative activities against varying degrees of hepatic damage. Therefore, DDB (100, 25, 5, or 50 mg/kg depending on the model) was administered to ...

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  • Functional analysis of the superoxide dismutase family in ...

    2010-2-9u2002·u2002SOD deletions showed that SOD4 is an essential gene. To analyse the functions of AfSOD1 to AfSOD4 in A. fumigatus, mutants were constructed by the replacement of each gene by a hygromycin‐resistance cassette in an akuB strain background. A correct and unique integration event of corresponding drug‐resistance gene was seen at the expected locus for SOD1‐3, as verified by PCR …

    Get Price
  • Jiuzhuan Huangjing Pills relieve mitochondrial dysfunction ...

    2021-10-1u2002·u2002Background. Metabolic dysfunction-associated fatty liver disease (MAFLD) is a common global chronic liver disease. Jiuzhuan Huangjing Pills (JHP) have been used for the treatment of human disease for over a thousand years, but their efficacy and underlying mechanism(s) of action against MAFLD are unknown.

    Get Price
  • Regulation of Heat Shock Proteins 27 and 70, p-Akt/p

    2013-12-6u2002·u2002After completion of the reperfusion period, blood samples were withdrawn from the heart and serum was separated via centrifugation (Heraeus Biofuge, Germany) at 3000g for 5 min. Thereafter, animals were sacrificed under an overdose of anesthesia (pentobarbitone sodium 100 mg/kg, i.v.), and their hearts were excised and processed for ...

    Get Price
  • Enhancer of zeste homolog 2 modulates oxidative stress ...

    2019-11-27u2002·u2002Kidney function was assessed by detecting serum creatinine (Cr) and blood urea nitrogen (BUN) of 2 mL blood from mice using commercial kit in accordance with the manufacturer's instructions (Nanjing Jiancheng Co., China). 2.7 Superoxide dismutase and malondialdehyde measurement

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  • Frontiers

    2020-11-27u2002·u2002Many studies proposed that oxidative stress and apoptosis are key mechanisms in the pathogenesis of contrast-induced acute kidney injury (CI-AKI). Xylose-pyrogallol conjugate (XP) is an original effective antioxidant that showed decent antioxidant and anti-apoptosis effect before. Thus the therapeutic effect and mechanism of XP in preventing CI-AKI in the short and long term were …

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  • Resveratrol and its derivative pterostilbene attenuate ...

    1. Introduction. Oxidative stress, an imbalance between the formation of free radicals and the intracellular antioxidative system, is a common and critical event in the initiation and development of intestinal diseases, such as ischemic intestinal injury, inflammatory bowel diseases, and even colorectal cancers [].Oxidative stress destroys the intestinal structure and induces apoptosis and ...

    Get Price
  • Consumption of hydrogen-rich water alleviates renal injury ...

    2014-3-21u2002·u2002In hypertensive animals and patients, oxidative stress represents the primary risk factor for progression of renal disease. Recently, it has been demonstrated that hydrogen, as a novel antioxidant, can selectively reduce hydroxyl radicals and peroxynitrite anion to exert therapeutic antioxidant activity. Herein, we investigated the protective effect of hydrogen-rich water (HW) against renal ...

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  • Superoxide Flux in Endothelial Cells via the Chloride ...

    2007-3-14u2002·u2002Reactive oxygen species (ROS) have been implicated in both cell signaling and pathology. A major source of ROS in endothelial cells is NADPH oxidase, which generates superoxide (O2.−) on the extracellular side of the plasma membrane but can result in intracellular signaling. To study possible transmembrane flux of O2.−, pulmonary microvascular endothelial cells were preloaded with the O2 ...

    Get Price
  • Protection of Pentoxifylline against Testis Injury Induced ...

    PTX was administered for 32 days. Blood and tissue samples were collected 7 days thereafter. Serum malondialdehyde levels were used to assess lipid peroxidation; ferric-reducing antioxidant power (FRAP), superoxide dismutase, and catalase and glutathione peroxidase enzyme activities were assessed to determine antioxidant capacity in various ...

    Get Price
  • Role of dopamine D2 receptors in ischemia/reperfusion ...

    2011-2-16u2002·u2002Myocardial ischemia/reperfusion injury is the major cause of morbidity and mortality for cardiovascular diseases. Dopamine D2 receptors are expressed in cardiac tissues. However, the roles of dopamine D2 receptors in myocardial ischemia/reperfusion injury and cardiomyocyte apoptosis are unclear. Here we investigated the effects of both dopamine D2 receptors agonist (bromocriptine) and ...

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  • Prevention of Udder Edema in Dairy Cows – DAIReXNET

    2019-8-16u2002·u2002Pitzen (1993) has employed the prooxidant-to-antioxidant ratio (PAR) to minimize oxidative stress and related problems in dairy cows. The PAR increases when prooxidants such as Fe exceed antioxidants and cofactors such as vitamin E, β-carotene, Cu, Zn, Se, and Mn. Pitzen's (1993) approach is to lower prooxidant intake when possible, but when ...

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  • Functional analysis of the superoxide dismutase family in ...

    2010-2-9u2002·u2002SOD deletions showed that SOD4 is an essential gene. To analyse the functions of AfSOD1 to AfSOD4 in A. fumigatus, mutants were constructed by the replacement of each gene by a hygromycin‐resistance cassette in an akuB strain background. A correct and unique integration event of corresponding drug‐resistance gene was seen at the expected locus for SOD1‐3, as verified by PCR …

    Get Price
  • :: JVS :: Journal of Veterinary Science

    Superoxide dismutase (SOD) activity in erythrocytes was determined based on the ability of SOD to inhibit the reduction of nitro-blue tetrazolium (NBT) by superoxide . One unit of SOD activity is defined as the amount of enzyme that causes a reduction of half the maximum inhibition of NBT; this reduction is obtained by measuring the absorbance ...

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  • Advances in Experimental and Clinical Research of the ...

    2021-10-20u2002·u2002Gouty arthritis (GA) is a multifactorial disease whose pathogenesis is utterly complex, and the current clinical treatment methods cannot wholly prevent GA development. Western medicine is the primary treatment strategy for gouty arthritis, but it owns an unfavorable prognosis. Therefore, the prevention and treatment of GA are essential. In China, traditional Chinese medicine (TCM) has been ...

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  • Reference Studies – Deerland Probiotics and Enzymes

    Antioxidant enzymes, such as superoxide dismutase (SOD), are candidate drugs for controlling this. This study from 2004 was designed to determine the therapeutic value of antioxidant enzymes in an experimental model of colitis, and to study the mechanisms …

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  • Superoxide dismutase mimic - Duke University

    37. A mimic of superoxide dismutase activity comprising an atom of manganese having an actual valence of 3 complexed with three hydroxamate groups. 38. The mimic of superoxide dismutase activity according to claiim 37 wherein said hydroxamate groups are derived from a single molecule of desferrioxamine. 39.

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  • Molecular Pharmacology, Department of Biofunctional ...

    reduction. In wet AMD, abnormal blood vessels grow beneath the retina forming a neovascular membrane. These vessels can leak fluid and blood that cause swelling and damage of the photoreceptors in the macular area. The pathogenesis of AMD is multifactorial and many genetic and environmental factors have been implicated. The well-known

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  • Resveratrol and its derivative pterostilbene attenuate ...

    1. Introduction. Oxidative stress, an imbalance between the formation of free radicals and the intracellular antioxidative system, is a common and critical event in the initiation and development of intestinal diseases, such as ischemic intestinal injury, inflammatory bowel diseases, and even colorectal cancers [].Oxidative stress destroys the intestinal structure and induces apoptosis and ...

    Get Price
  • Superoxide Flux in Endothelial Cells via the Chloride ...

    2007-3-14u2002·u2002Reactive oxygen species (ROS) have been implicated in both cell signaling and pathology. A major source of ROS in endothelial cells is NADPH oxidase, which generates superoxide (O2.−) on the extracellular side of the plasma membrane but can result in intracellular signaling. To study possible transmembrane flux of O2.−, pulmonary microvascular endothelial cells were preloaded with the O2 ...

    Get Price
  • Protection of Pentoxifylline against Testis Injury Induced ...

    PTX was administered for 32 days. Blood and tissue samples were collected 7 days thereafter. Serum malondialdehyde levels were used to assess lipid peroxidation; ferric-reducing antioxidant power (FRAP), superoxide dismutase, and catalase and glutathione peroxidase enzyme activities were assessed to determine antioxidant capacity in various ...

    Get Price
  • Up-Regulation of Superoxide Dismutase 2 in 3D Spheroid ...

    Superoxide dismutase activity was measured using the superoxide dismutase assay kit, according to the manufacturer's instructions (Sigma). SOD2 activity was measured in the presence of a copper/zinc superoxide dismutase inhibitor (Cu/Zn SOD). Data were normalized to protein content. Absorbance values were measured at 450 nm.

    Get Price
  • Assay of superoxide dismutase activity in a plate assay ...

    2016-12-1u2002·u2002One of the most convenient methods for measuring superoxide dismutase activity is a plate assay using xanthine oxidase and the water soluble tetrazolium WST-1.

    Get Price
  • Conjugates of Superoxide Dismutase 1 with Amphiphilic

    The enzyme superoxide dismutase 1 (SOD1, also known as Cu,Zn/SOD) efficiently catalyzes the dismutation of superoxide into oxygen and hydrogen peroxide. It is a stable homodimer of identical 16 kDa subunits held together primarily by hydrophobic interactions and cofactored with copper and zinc 8, …

    Get Price
  • Ceria-based nanotheranostic agent for rheumatoid arthritis

    2020-10-25u2002·u2002Treatment of rheumatoid arthritis by targeting macrophages through folic acid tailored superoxide dismutase and serratiopeptidase. J Drug Deliv Sci Technol. 2017;41:431-435 50. Younus H. Therapeutic potentials of superoxide dismutase. Int J Health Sci. 2018;12:88-93 51. Tan H-Y, Wang N, Li S, Hong M, Wang X, Feng Y.

    Get Price
  • Autophagy and mitochondrial dysfunction in adjuvant ...

    2016-9-9u2002·u2002Superoxide anion is regulated by enzymes such as superoxide dismutase (SOD) and peroxidases, as well as endogenous supplies of antioxidants such as glutathione (GSH) 40. Resveratrol is a phenolic compound, which is widely found in various fruits and vegetables, possess antioxidant, anti-aging, regulation of lipid metabolism, anti-cancer ...

    Get Price
  • Changes of stress oxidative enzymes in rat mammary

    Blood samples u200ewere u200ecentrifuged at 2,000 g for 10 min at 4 ˚C u200eu200f)u200fModel 5804 centrifuge; Eppendorf AG, Hamburg, Germanyu200e)u200e to obtain serum and stored in – 20 ˚C until enzyme analysis.u200e The procedures were u200eperformed according to u200ethe u200emanufacturer's protocol like u200ethe mammary tissues supernatant.

    Get Price
  • Tumor suppressive activity of a variant isoform of ...

    This led to the previously reported observation that a liposarcoma‐derived cell line (LSA), released (a) factor(s) able to kill some tumor‐derived mammary epithelial cells, while sparing the corresponding normal cells. 11, 12, 13 In the present investigation, a variant form of human manganese superoxide dismutase (MnSOD) was identified as a ...

    Get Price
  • Protective Effect of Melatonin and Catalase in Bovine ...

    Superoxide production by bovine neutrophils. Neutrophils (1 × 10 6 /ml) were incubated in presence or absence of lipopolysaccharide (LPS) (1 μ g/ml) for 48 hrs at 37 ° C and 5% CO 2 .

    Get Price
  • Autophagy and mitochondrial dysfunction in adjuvant ...

    2016-9-9u2002·u2002Resveratrol is a polyphenol derivatives which exhibits a pro-apoptotic effect in a variety of human cancers by triggering mitochondria apoptosis pathway and autophagy. However, there are …

    Get Price
  • The protective effects of Ribes diacanthum Pall on ...

    2016-2-3u2002·u2002Ethnopharmacological relevance. Ribes diacanthum Pall. (Saxifragaceae), a Mongolian folk medicinal plant, was used to treat urinary system diseases. The present work aims to investigate the protective effects of Ribes diacanthum Pall (RDP) against cisplatin-induced nephrotoxicity.

    Get Price