germany calf ossotide promote cell growth factory

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  • Thermo Scientific HyClone FetalClone I, II and III: Serum ...

    2013-10-22u2002·u2002bovine calf serum on 6 cell types. Data demonstrates that these alternatives match or outperform FBS in cell culture growth promotion in many applications. As these products are made from raw materials more readily available than FBS, they are recommended as cost effective alternatives to standard FBS. Introduction

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  • Bovine Calf Serum USA origin, for cell culture, sterile ...

    Animal serum is commonly used to supplement basal media formulations for the optimal growth of many cell types in vitro. Bovine Calf Serum (BCS) is collected from calves that are less than 12 months old. The serum contains approximately double the protein of fetal bovine serum (FBS). BCS will promote growth in a variety of mammalian cell lines ...

    Get Price
  • Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

    As a putative autologous replacement for animal-derived supplements such as fetal calf serum (FCS), PRP has been applied as cell culture supplement for the expansion of stem and progenitor cells for tissue engineering applications and cell therapies. Attributed to the high content of growth factors …

    Get Price
  • Cell Culture Serum Products

    Serum is added to what's called basal media to promote growth and maintenance of eukaryotic cells, including mammalian and insect cell lines. This common cell culture supplement provides nutrients to cells. Among these are hormones, growth factors, and minerals. Serum is typically added to basal media to a final concentration of 5% of 10%.

    Get Price
  • Animal‐cell culture media: History, characteristics, and ...

    2017-3-21u2002·u20021. Introduction. The influence of cell culture technology on human society has been immeasurable. Progress in biology in recent years, for example, has depended heavily on cell culture technology. 1 In addition, cell culture‐based practical technologies have been developed in various areas, including the assessment of the efficacy and toxicity of new drugs, manufacture of vaccines and ...

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  • Sensitization of epithelial growth factor receptors by ...

    2011-11-15u2002·u2002In comparison, KP372-1 had no negative effect on nicotine-mediated growth promotion. Again, concurrent treatment with KP372-1 and PD168393 completely blocked the nicotine-mediated effect on the growth of MCF10A and MDA-MB-231 cells. The data further supported the notion that nicotine may sensitize EGFR/ERK1/2/E2F1 signaling to promote cell growth.

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  • Boehringer Ingelheim - Value Through Innovation

    2 days agou2002·u2002Boehringer Ingelheim is one of the world's leading research-driven pharmaceutical companies with 130 years of experience.

    Get Price
  • Sensitization of epithelial growth factor receptors by ...

    2018-8-17u2002·u2002Sensitization of Epithelial Growth Factor Receptors by Nicotine Exposure to Promote Breast Cancer Cell Growth The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Nishioka, Takashi, Hyun-Seok Kim, Ling-Yu Luo, Yi Huang, Jinjin Guo, and Chang Yan Chen. 2011.

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  • Tumor-associated macrophages promote ovarian cancer

    2020-4-20u2002·u2002Steitz, A.M., Steffes, A., Finkernagel, F. et al. Tumor-associated macrophages promote ovarian cancer cell migration by secreting transforming growth factor beta induced (TGFBI) and …

    Get Price
  • Bioprocessing strategies for the large-scale production of ...

    2015-11-23u2002·u2002Human mesenchymal stem cells (hMSCs), also called mesenchymal stromal cells, have been of great interest in regenerative medicine applications because of not only their differentiation potential but also their ability to secrete bioactive factors that can modulate the immune system and promote tissue repair. This potential has initiated many early-phase clinical studies for the treatment of ...

    Get Price
  • Thermo Scientific HyClone FetalClone I, II and III: Serum ...

    2013-10-22u2002·u2002bovine calf serum on 6 cell types. Data demonstrates that these alternatives match or outperform FBS in cell culture growth promotion in many applications. As these products are made from raw materials more readily available than FBS, they are recommended as cost effective alternatives to standard FBS. Introduction

    Get Price
  • Bovine Calf Serum USA origin, for cell culture, sterile ...

    Animal serum is commonly used to supplement basal media formulations for the optimal growth of many cell types in vitro. Bovine Calf Serum (BCS) is collected from calves that are less than 12 months old. The serum contains approximately double the protein of fetal bovine serum (FBS). BCS will promote growth in a variety of mammalian cell lines ...

    Get Price
  • Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

    As a putative autologous replacement for animal-derived supplements such as fetal calf serum (FCS), PRP has been applied as cell culture supplement for the expansion of stem and progenitor cells for tissue engineering applications and cell therapies. Attributed to the high content of growth factors …

    Get Price
  • Cell Culture Serum Products

    Serum is added to what's called basal media to promote growth and maintenance of eukaryotic cells, including mammalian and insect cell lines. This common cell culture supplement provides nutrients to cells. Among these are hormones, growth factors, and minerals. Serum is typically added to basal media to a final concentration of 5% of 10%.

    Get Price
  • Animal‐cell culture media: History, characteristics, and ...

    2017-3-21u2002·u20021. Introduction. The influence of cell culture technology on human society has been immeasurable. Progress in biology in recent years, for example, has depended heavily on cell culture technology. 1 In addition, cell culture‐based practical technologies have been developed in various areas, including the assessment of the efficacy and toxicity of new drugs, manufacture of vaccines and ...

    Get Price
  • Sensitization of epithelial growth factor receptors by ...

    2011-11-15u2002·u2002In comparison, KP372-1 had no negative effect on nicotine-mediated growth promotion. Again, concurrent treatment with KP372-1 and PD168393 completely blocked the nicotine-mediated effect on the growth of MCF10A and MDA-MB-231 cells. The data further supported the notion that nicotine may sensitize EGFR/ERK1/2/E2F1 signaling to promote cell growth.

    Get Price
  • Boehringer Ingelheim - Value Through Innovation

    2 days agou2002·u2002Boehringer Ingelheim is one of the world's leading research-driven pharmaceutical companies with 130 years of experience.

    Get Price
  • Sensitization of epithelial growth factor receptors by ...

    2018-8-17u2002·u2002Sensitization of Epithelial Growth Factor Receptors by Nicotine Exposure to Promote Breast Cancer Cell Growth The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Nishioka, Takashi, Hyun-Seok Kim, Ling-Yu Luo, Yi Huang, Jinjin Guo, and Chang Yan Chen. 2011.

    Get Price
  • Tumor-associated macrophages promote ovarian cancer

    2020-4-20u2002·u2002Steitz, A.M., Steffes, A., Finkernagel, F. et al. Tumor-associated macrophages promote ovarian cancer cell migration by secreting transforming growth factor beta induced (TGFBI) and …

    Get Price
  • Bioprocessing strategies for the large-scale production of ...

    2015-11-23u2002·u2002Human mesenchymal stem cells (hMSCs), also called mesenchymal stromal cells, have been of great interest in regenerative medicine applications because of not only their differentiation potential but also their ability to secrete bioactive factors that can modulate the immune system and promote tissue repair. This potential has initiated many early-phase clinical studies for the treatment of ...

    Get Price
  • FRMD8 promotes inflammatory and growth factor signalling ...

    2018-6-13u2002·u2002Cells in the human body communicate with one another for many different reasons, including to help organs develop correctly and to produce a healthy reponse to injury and infection. Signalling proteins, such as growth factors and cytokines, form the main language of this communication. Initially, many growth factors and cytokines remain attached to the surface of the cell …

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  • Research Article Artificial Extracellular Matrices with ...

    2019-7-31u2002·u2002aECM will depend on the maturation state of the cell (line)s. TNAP activity and calcium deposition were determined as markers for osteogenic di erentiation. 2. Materials and Methods Unless otherwise mentioned, cell culture reagents were from Biochrom KG (Berlin Germany); fetal calf serum was from BioWest (via .Geyer, Hamburg, Germany); cell culture

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  • Complement Component C3 and Complement Factor B Promote ...

    2017-5-1u2002·u2002cSCC cell cultures were transfected with 75 nmol/L C3 siRNAs, CFB siRNAs, or control siRNA and incubated in complete growth medium for 24 hours to reach confluency. Cell division was inhibited with 1 mmol/L hydroxyurea (Sigma Aldrich) treatment in Dulbecco's modified Eagle's medium with 10% fetal calf serum for 16 hours.

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  • Effects of thalidomide on growth and VEGF‑A expression in ...

    essential for tumor growth and metastasis. Vascular endothelial growth factor (VEGF) particularly VEGF-A is important in the process of angiogenesis. The current research has indicated that thalidomide (THD) may be able to inhibit angiogenesis, stimulate the activity of the immune system and inhibit the adherence of cancer cells to stromal cells.

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  • Mesenchymal stem cells promote endothelial progenitor cell ...

    2017-6-9u2002·u2002Bone marrow mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) interact with each other. EPCs are able to promote the self‑renewal of MSCs as niche cells in murine bone marrow, and MSCs are able to promote EPC proliferation in vitro in a co‑culture system. It has previously been reported that MSCs can secrete insulin‑like growth factor‑1 (IGF‑1), which …

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  • The role of fibrinolysis inhibition in engineered vascular ...

    2018-9-26u2002·u2002sign is crucial to promote cell growth and regeneration. In general, the scaffold used should match the mechan-ical properties of the target tissue and its rate of degrad-ation should be fine-tuned with the respective tissue growth rate [10]. In this context, fibrin is a suitable ma-terial for soft tissue engineering, since its mechanical

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  • Inhibiting lysine 353 oxidation of GRP78 by a hypochlorous ...

    2019-11-12u2002·u2002To explore the effect of ZBM-H on cancer cell growth, we firstly examined the morphological changes in A549 cells after treatment with ZBM-H at 1, 2, 5, and 10 μM for 3, 6, 12, 24 and 48 h using ...

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  • Article Distinct Effect of CD40 and TNF-Signaling on the ...

    2013-4-25u2002·u2002signaling can promote a protective CD8 T cell mediated immunity that is independent of CD4 T cell help (17). We previously observed that CD40/CD40L signal could facilitate DC growth and maturation, antagonize the negative effect of IL-10, and reinforce DC ability to accelerate T cell activation and expansion (18).

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  • Milk Facts: What about Humanely Raised Milk and Dairy ...

    Cows produce an average of 729 days of milk, which corresponds to 2.4 lactations, before they are considered 'spent.' 6. Dairy cows are marketed and sold to slaughterhouses. While cows can live up to 25 years, dairy cows are typically removed from the dairy herd at age 2-5 when their milk production weans, their lives cut drastically short ...

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  • Osteopontin regulates proliferation, migration, and ...

    2021-9-12u2002·u2002Osteopontin regulates cell growth, cell adhesion, homeostasis, migration, and survival of various cell types. ... abundant cells in the CNS and play significant roles in health and disease. Astrocytes are involved in homeostasis, promote neuroprotection, and regulate synaptic plasticity. ... with the addition of 10% fetal calf serum (FCS), 1% ...

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  • Blood vessel maturation in a 3‐dimensional spheroidal ...

    2001-2-1u2002·u2002Cell culture. Endothelial cell growth medium (ECGM), endothelial cell growth supplement (human umbilical vein endothelial cell culture), and human smooth muscle cell growth medium (HSMCGM) were purchased from Promocell (Heidelberg, Germany). Fetal calf serum (FCS) was obtained from Biochrom (Berlin, Germany).

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  • Strontium ions promote in vitro human bone marrow

    2018-12-25u2002·u2002The effect of solely strontium ions—with only calcium in the media resulting from fetal calf serum—was investigated. Commercially available calcium‐free medium (modified α‐MEM) was tested in comparison with media with varied calcium ion concentrations (0.9, 1.8, and 3.6 mM), or strontium ion concentration (0.4, 0.9, 1.8, and 3.6 mM).

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  • Synthetic osteogenic growth peptide promotes ...

    2011-7-8u2002·u2002The osteogenic growth peptide (OGP) is a naturally occurring tetradecapeptide identical to the C-terminal amino acid sequence 89–102 of histone H4 (H4) [].OGP increases bone formation and trabecular bone density [].In cultures of osteoblastic and other bone marrow stromal cells derived from human and other mammalian species, OGP stimulates proliferation, alkaline phosphatase activity, and ...

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  • Mesenchymal stem cells promote endothelial progenitor

    2017-6-9u2002·u2002Bone marrow mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) interact with each other. EPCs are able to promote the self‑renewal of MSCs as niche cells in murine bone marrow, and MSCs are able to promote EPC proliferation in vitro in a co‑culture system. It has previously been reported that MSCs can secrete insulin‑like growth factor‑1 (IGF‑1), which serves ...

    Get Price
  • Dynamic flow priming programs allow tuning up the cell ...

    2021-7-19u2002·u2002Frozen stock of each types of the cells was thawed and scaled up in appropriate media: D-MEM (High Glucose with Phenol Red and Sodium Pyruvate, StemSure, Wako) supplemented with 10% of fetal calf ...

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  • Osteocyte-derived exosomes induced by mechanical strain ...

    2020-7-17u2002·u2002The oral cavity is a complex environment in which periodontal tissue is constantly stimulated by external microorganisms and mechanical forces. Proper mechanical force helps maintain periodontal tissue homeostasis, and improper inflammatory response can break the balance. Periodontal ligament (PDL) cells play crucial roles in responding to these challenges and maintaining the homeostasis of ...

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  • A User's Guide to Cell-Free Protein Synthesis

    2019-3-12u2002·u20021. Introduction. Cell-free protein synthesis (CFPS) emerged about 60 years ago as a platform used by Nirenberg and Matthaei to decipher the genetic code and discover the link between mRNA and protein synthesis [].Since this discovery, the CFPS platform has grown to enable a variety of applications, from functional genomics to large-scale antibody production [2,3].

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  • Inner nuclear protein Matrin-3 coordinates cell ...

    2021-10-29u2002·u2002During MEL cell differentiation, cell size is normally reduced (p = 0.002, by t test); the effect is more pronounced in Matr3 KO cells (p = 0.0004, by two-sided t test).

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  • Sildenafil Potentiates a cGMP-Dependent Pathway to

    Use of the phosphodiesterase 5 inhibitor sildenafil (Viagra) has been linked to an increased risk of melanoma. Dhayade et al. explore the underlying mechanism and identify a growth-promoting cGMP-MAPK pathway in melanoma cells that is potentiated by sildenafil treatment.

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  • Thermo Scientific HyClone FetalClone I, II and III: Serum ...

    2013-10-22u2002·u2002bovine calf serum on 6 cell types. Data demonstrates that these alternatives match or outperform FBS in cell culture growth promotion in many applications. As these products are made from raw materials more readily available than FBS, they are recommended as cost effective alternatives to standard FBS. Introduction

    Get Price
  • Bovine Calf Serum USA origin, for cell culture, sterile ...

    Animal serum is commonly used to supplement basal media formulations for the optimal growth of many cell types in vitro. Bovine Calf Serum (BCS) is collected from calves that are less than 12 months old. The serum contains approximately double the protein of fetal bovine serum (FBS). BCS will promote growth in a variety of mammalian cell lines ...

    Get Price
  • Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

    As a putative autologous replacement for animal-derived supplements such as fetal calf serum (FCS), PRP has been applied as cell culture supplement for the expansion of stem and progenitor cells for tissue engineering applications and cell therapies. Attributed to the high content of growth …

    Get Price
  • Cell Culture Serum Products

    Serum is added to what's called basal media to promote growth and maintenance of eukaryotic cells, including mammalian and insect cell lines. This common cell culture supplement provides nutrients to cells. Among these are hormones, growth factors, and minerals. Serum is typically added to basal media to a final concentration of 5% of 10%.

    Get Price
  • Animal‐cell culture media: History, characteristics, and ...

    2017-3-21u2002·u20021. Introduction. The influence of cell culture technology on human society has been immeasurable. Progress in biology in recent years, for example, has depended heavily on cell culture technology. 1 In addition, cell culture‐based practical technologies have been developed in various areas, including the assessment of the efficacy and toxicity of new drugs, manufacture of …

    Get Price
  • Sensitization of epithelial growth factor receptors by ...

    2011-11-15u2002·u2002In comparison, KP372-1 had no negative effect on nicotine-mediated growth promotion. Again, concurrent treatment with KP372-1 and PD168393 completely blocked the nicotine-mediated effect on the growth of MCF10A and MDA-MB-231 cells. The data further supported the notion that nicotine may sensitize EGFR/ERK1/2/E2F1 signaling to promote cell growth.

    Get Price
  • Boehringer Ingelheim - Value Through Innovation

    2 days agou2002·u2002Boehringer Ingelheim is one of the world's leading research-driven pharmaceutical companies with 130 years of experience.

    Get Price
  • Sensitization of epithelial growth factor receptors by ...

    2018-8-17u2002·u2002Sensitization of Epithelial Growth Factor Receptors by Nicotine Exposure to Promote Breast Cancer Cell Growth The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Nishioka, Takashi, Hyun-Seok Kim, Ling-Yu Luo, Yi Huang, Jinjin Guo, and Chang Yan Chen. 2011.

    Get Price
  • Tumor-associated macrophages promote ovarian cancer cell ...

    2020-4-20u2002·u2002Steitz, A.M., Steffes, A., Finkernagel, F. et al. Tumor-associated macrophages promote ovarian cancer cell migration by secreting transforming growth factor beta induced (TGFBI) and …

    Get Price
  • Bioprocessing strategies for the large-scale production of ...

    2015-11-23u2002·u2002Human mesenchymal stem cells (hMSCs), also called mesenchymal stromal cells, have been of great interest in regenerative medicine applications because of not only their differentiation potential but also their ability to secrete bioactive factors that can modulate the immune system and promote tissue repair. This potential has initiated many early-phase clinical studies for the …

    Get Price
  • Human Platelet Lysate Can Replace Fetal Calf Serum as a ...

    Fetal calf serum (FCS) is frequently used as a growth factor and protein source in bone-marrow-derived mesenchymal stromal cell (BMSC) culture media, although it is a xenogenic product presenting multiple disadvantages including but not limited to ethical concerns. A promising alternative for FCS is …

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  • Cell Culture and Uses for Fetal Bovine Serum – Innovative ...

    Also known as ' fetal calf serum ' FBS is widely used in cell culture applications because of its numerous growth factors, and low level of antibodies. At Innovative Research, we provide only the highest quality, US Origin, fetal bovine serum and other biomaterials used for in-vitro research purposes today. Innovative Research was established ...

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  • Media and Supplements in Cell Culture - Sigma-Aldrich

    Serum is a complex mix of albumins, growth factors and growth inhibitors and is probably one of the most important components of cell culture medium. The most commonly used serum is fetal bovine serum (FBS). Other types of serum are available including newborn calf serum and horse serum.

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  • Glial Cell Line-Derived Neurotrophic Factor Requires ...

    1998-11-11u2002·u2002Glial Cell Line-Derived Neurotrophic Factor Requires Transforming Growth Factor-bfor Exerting Its Full Neurotrophic Potential onPeripheral and CNS Neurons Kerstin Krieglstein,1 Prisca Henheik,1 Lilla Farkas,1 Jozsef Jaszai,1 Dagmar Galter,1 Knut Krohn2, and Klaus Unsicker1 1Department of Neuroanatomy, University of Heidelberg, D-69120 Heidelberg, Germany and …

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  • Activation of Vascular Endothelial Growth Factor Receptor ...

    2003-11-5u2002·u2002cells (BRECs) were isolated from freshly isolated calf eyes by homogenization and a series of filtration steps, as described previously (19). Primary BRECs were cultured in microvascular endothelial cell basal medium (MECBM) with growth supplement (TCS CellWork, Buckingham, U.K.) in fibronectin-coated dishes.

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  • Leaf extracts from Nitraria retusa promote cell population ...

    2011-10-31u2002·u2002Cells were cultivated in RPMI-1640 medium supplemented with 10% (v/v) foetal calf serum, 0.1 mg/ml gentamicin and 2 mM L-glutamine as a complete growth medium and were incubated at 37)°C in an incubator with 5% CO 2 in a humidified atmosphere. Every two days the cells were subcultured by splitting the culture with fresh medium.

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  • Improving the in vivo Persistence, Distribution and ...

    intensity and cell cycle of cytotoxic T lymphocytes distributed in various organs, in comparison with tumor growth. We found down-regulating Tregs in vivo can simultaneously reduce the levels of interleukin-10 and transforming growth factor-b. Migration and distribution of cytotoxic T lymphocytes in vivo was found to vary with time.

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  • Feeding calves: milk replacer or whole milk?

    2020-3-9u2002·u2002Feeding whole milk can result is calves being infected with diseases, such as Johne's, that afflicted cows from which the milk was obtained. Waste milk feeding can also lead to the potential spread of E.coli from cow to calf. Both of these scenarios can result in significant long- and short-term harm to calf health, growth rates, and performance.

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  • The effect of culture media on large-scale expansion and ...

    2019-8-5u2002·u2002Mesenchymal stromal cells (MSC) have been isolated from various tissues and organs throughout the human body [].The exceptional ease of their isolation and ex vivo expansion additional to their ascribed regeneration potential led to the extremely broad application of MSC in a clinical trial [] and subsequently led them to clinical medicine as advanced therapy medicinal …

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  • Type IV collagen stimulates pancreatic cancer cell ...

    2013-3-26u2002·u2002Pancreatic cancer cells were grown on both type I or type IV collagen matrices, and both types of collagen promote cell growth when compared to a control protein matrix (Figure 4B). Furthermore, there was a tendency of having reduced induction of apoptosis when the pancreatic cancer cells were grown on collagen matrices (Figure 4 C).

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