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  • Bovine Calf Serum USA origin, for cell culture, sterile ...

    Animal serum is commonly used to supplement basal media formulations for the optimal growth of many cell types in vitro. Bovine Calf Serum (BCS) is collected from calves that are less than 12 months old. The serum contains approximately double the protein of fetal bovine serum (FBS). BCS will promote growth in a variety of mammalian cell lines ...

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  • Bovine Serum (Calf Serum)

    Bovine Serum (Calf Serum) Gibco® bovine (aka calf serum) serum is collected from prime cattle with an age range of 12-36 months, but typically less than 24 months. All lots are processed and manufactured in New Zealand. Each lot of bovine serum is tested for its ability to support the growth of VERO cells over three subcultures.

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  • Calf Bovine Serum, Iron Fortified

    Iron supplemented and triple filtered through 0.1 μm filters. Each lot of calf bovine serum is tested for sterility and for the ability to support the growth of cells using sequential growth curves. Calf bovine serum is manufactured from bovine blood collected in USDA-inspected abattoirs located in the United States. Serum has not come from cattle born, raised, shipped through, or slaughtered ...

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  • Cell Culture and Uses for Fetal Bovine Serum – Innovative ...

    Also known as ' fetal calf serum ' FBS is widely used in cell culture applications because of its numerous growth factors, and low level of antibodies. At Innovative Research, we provide only the highest quality, US Origin, fetal bovine serum and other biomaterials used for in-vitro research purposes today. Innovative Research was established ...

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  • Long-term growth comparison studies of FBS and FBS ...

    2017-6-7u2002·u2002Many experiments require plating cells at low density so that individual colonies can develop. Therefore, the plating efficiency assay is used by cell culture scientists to test the ability of serum to promote cell growth. The head and neck cell lines cultured in six …

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  • Thermo Scientific HyClone FetalClone I, II and III: Serum ...

    2013-10-22u2002·u2002bovine calf serum on 6 cell types. Data demonstrates that these alternatives match or outperform FBS in cell culture growth promotion in many applications. As these products are made from raw materials more readily available than FBS, they are recommended as cost effective alternatives to standard FBS. Introduction

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  • Phospho-eEF2k (Ser366) Antibody - Cell Signaling

    IP. Western blot analysis of extracts from SW-13 cells (starved for 18 hours) treated with calf intestinal alkaline phosphatase (CIP) or 20% fetal bovine serum for 30 minutes, and extracts from HeLa cells (starved for 18 hours) treated with 200 nM TPA for 30 minutes, using Phospho-eEF2k (Ser366) Antibody (upper) or eEF2k Antibody #3692 (lower).

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  • Biomaterials that promote cell-cell interactions enhance ...

    2017-9-1u2002·u2002MSCs isolated from the tibiae of Sprague Dawley rats via bone marrow biopsies were used in this study. Cell culture was carried out in full culture media i.e. low glucose DMEM containing fetal calf serum (10%), penicillin/streptomycin (1%), and Glutamax™ (1%). Cells were passaged at approximately 70% confluency, and were not used beyond ...

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  • Mechanical loading promotes Lewis lung cancer cell

    2009-6-16u2002·u2002Mechanical loading is known to trigger proliferation of tumor cells. Periostin is a new molecule found to increase in many cancers. To determine how mechanical strain modulates tumor growth and its possible mediation by periostin through TGF-β1, Lewis lung cancer cells were cultured on flexible-bottomed culture plates and cyclically strained using Flexercell Strain Unit. Real-time RT-PCR …

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  • Tumor-associated macrophages promote ovarian cancer

    2020-4-20u2002·u2002Steitz, A.M., Steffes, A., Finkernagel, F. et al. Tumor-associated macrophages promote ovarian cancer cell migration by secreting transforming growth factor beta induced (TGFBI) and …

    Get Price
  • Cell Culture Serum Products

    Serum is added to what's called basal media to promote growth and maintenance of eukaryotic cells, including mammalian and insect cell lines. This common cell culture supplement provides nutrients to cells. Among these are hormones, growth factors, and minerals. Serum is typically added to basal media to a final concentration of 5% of 10%.

    Get Price
  • Animal‐cell culture media: History, characteristics, and ...

    2017-3-21u2002·u2002Other kinds of serum are used in certain situations, including calf serum (CS) and horse serum. Researchers can select an appropriate serum type on the basis of its characteristics. Fetal bovine serum generally is rich in growth factors and contains low levels of γ‐globulins (which have a cell growth‐inhibitory activity).

    Get Price
  • Nano‑hydroxyapatite/collagen film as a favorable substrate ...

    the intention to preserve cell phenotype and promote cell growth to ensure sufficient and functional cells, this study may provide novel insights for cell-based therapy. Materials and methods Materials and chemicals. colia 1 of calf origin and nano -Ha 2 powder were …

    Get Price
  • Biomaterials that promote cell-cell interactions enhance ...

    2017-9-1u2002·u2002MSCs isolated from the tibiae of Sprague Dawley rats via bone marrow biopsies were used in this study. Cell culture was carried out in full culture media i.e. low glucose DMEM containing fetal calf serum (10%), penicillin/streptomycin (1%), and Glutamax™ (1%). Cells were passaged at approximately 70% confluency, and were not used beyond ...

    Get Price
  • Research Article Artificial Extracellular Matrices with ...

    2019-7-31u2002·u2002via LGC Standards GmbH, Wesel, Germany) and cultured according to supplier s recommendations. MLO-Y cell line was described in detail by Kato et al. [ ] and was a kind gi from Lynda Bonewald (Kansas City, USA). Cell characteristics, plating density, and media used for cell expansion are given in Table . In order to have the di erent cell (line ...

    Get Price
  • Allergan: A Bold, Global Pharmaceutical Company

    Allergan plc (NYSE: AGN), headquartered in Dublin, Ireland, is a global pharmaceutical leader focused on developing, manufacturing and commercializing branded pharmaceutical, device, biologic, surgical and regenerative medicine products for patients around the world.

    Get Price
  • Research Article Endothelial Progenitor Cell Fraction ...

    2019-7-31u2002·u2002medium containing autologous growth factors (PL) that was showntopromoteMSCsdi erentiation[,]. 2. Materials and Methods.. Cell Culture Media. Iscove s Modi ed Dulbecco s Medium (IMDM), Fetal Calf Serum (FSC), Nonessential Amino Acids (NEAA), and antibiotics (PenStrep, PS) were purchased from Gibco/Invitrogen Life Technologies (Zug, Switzerland).

    Get Price
  • Cell Communication in a Coculture System Consisting of ...

    2014-4-22u2002·u2002It is thus possible to promote the metabolic activities of these two cell types within a coculture system. 100,000 primary osteoblasts were seeded at the lower surface and 66,000 OECs were seeded at the upper surface of a polycarbonate transmembrane filter in a Transwell filter system in a 24-well plate (pore size 0.4 μm; 0.588 cm 2 /filter ...

    Get Price
  • Tumor-associated macrophages promote ovarian cancer cell ...

    2020-4-20u2002·u2002Steitz, A.M., Steffes, A., Finkernagel, F. et al. Tumor-associated macrophages promote ovarian cancer cell migration by secreting transforming growth factor beta induced (TGFBI) and …

    Get Price
  • Sildenafil Potentiates a cGMP-Dependent Pathway to Promote ...

    Use of the phosphodiesterase 5 inhibitor sildenafil (Viagra) has been linked to an increased risk of melanoma. Dhayade et al. explore the underlying mechanism and identify a growth-promoting cGMP-MAPK pathway in melanoma cells that is potentiated by sildenafil treatment.

    Get Price
  • Bovine Serum (Calf Serum)

    Bovine Serum (Calf Serum) Gibco® bovine (aka calf serum) serum is collected from prime cattle with an age range of 12-36 months, but typically less than 24 months. All lots are processed and manufactured in New Zealand. Each lot of bovine serum is tested for its ability to support the growth of VERO cells over three subcultures.

    Get Price
  • Polygonum multiflorum extract support hair growth by ...

    2020-5-12u2002·u2002Human Dermal Papilla cells (DPCs) were purchased from Promocell (Promocell, Heidelberg, Germany). DPCs were cultured in a basal medium supplemented with Supple-ment Mix which contains 4% fetal calf serum, 0.4% bovine pituitary extract, 1ng/ml basic fibroblast growth factor, and 5μg/ml insulin. Cells were maintained in humidified incubatorat37 ...

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  • The role of fibrinolysis inhibition in engineered vascular ...

    2018-9-26u2002·u2002vested from the same individual. Furthermore, fetal calf serum (FCS) can be substituted with human platelet lys- ... the close contact of both cell types is re-quired for microvascular tube formation [9]. Scaffold de-sign is crucial to promote cell growth and regeneration. In general, the scaffold used should match the mechan- ... Germany) or ...

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  • Thermo Fisher Scientific - US

    Thermo Fisher Scientific enables our customers to make the world healthier, cleaner and safer.

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  • Accelerated osteoblast mineralization on a conductive ...

    2011-2-17u2002·u2002One of the major benefits of a conductive PPy-based substrate is that the mediated electrical stimulation (ES) can be a stimulating factor to promote tissue regeneration. We cultured osteoblast-like Saos-2 cells on a conductive substrate made of biodegradable polylactide (95 wt%) and electrically conducting polypyrrole bioactivated with heparin (PPy/HE) (5 wt%). Using multi-well electrical ...

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  • Mesenchymal stem cells promote endothelial progenitor

    2017-6-9u2002·u2002Bone marrow mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) interact with each other. EPCs are able to promote the self‑renewal of MSCs as niche cells in murine bone marrow, and MSCs are able to promote EPC proliferation in vitro in a co‑culture system. It has previously been reported that MSCs can secrete insulin‑like growth factor‑1 (IGF‑1), which serves ...

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  • In Vitro Endothelialization Test of Biomaterials Using ...

    2016-6-27u2002·u2002Functionalizing biomaterials with peptides or polymers that enhance recruitment of endothelial cells (ECs) can reduce blood coagulation and thrombosis. To assess endothelialization of materials in vitro, primary ECs are generally used, although the characteristics of these cells vary among the donors and change with time in culture. Recently, primary cell lines immortalized by transduction of ...

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  • Feeding calves: milk replacer or whole milk?

    2020-3-9u2002·u2002Feeding whole milk can result is calves being infected with diseases, such as Johne's, that afflicted cows from which the milk was obtained. Waste milk feeding can also lead to the potential spread of E.coli from cow to calf. Both of these scenarios can result in significant long- and short-term harm to calf health, growth rates, and performance.

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  • Research Article Endothelial Progenitor Cell Fraction ...

    2019-7-31u2002·u2002medium containing autologous growth factors (PL) that was showntopromoteMSCsdi erentiation[,]. 2. Materials and Methods.. Cell Culture Media. Iscove s Modi ed Dulbecco s Medium (IMDM), Fetal Calf Serum (FSC), Nonessential Amino Acids (NEAA), and antibiotics (PenStrep, PS) were purchased from Gibco/Invitrogen Life Technologies (Zug, Switzerland).

    Get Price
  • Sildenafil Potentiates a cGMP-Dependent Pathway to

    Use of the phosphodiesterase 5 inhibitor sildenafil (Viagra) has been linked to an increased risk of melanoma. Dhayade et al. explore the underlying mechanism and identify a growth-promoting cGMP-MAPK pathway in melanoma cells that is potentiated by sildenafil treatment.

    Get Price
  • Cell Culture and Uses for Fetal Bovine Serum – Innovative ...

    Also known as ' fetal calf serum ' FBS is widely used in cell culture applications because of its numerous growth factors, and low level of antibodies. At Innovative Research, we provide only the highest quality, US Origin, fetal bovine serum and other biomaterials used for in-vitro research purposes today. Innovative Research was established ...

    Get Price
  • Serum in the medium - The unknown component -

    2019-8-22u2002·u2002August 2019. Serum is a common supplement in media used for cultivating animal cells. Fetal bovine serum (FBS), also known as fetal calf serum (FCS), is the most widely used. It contains vital nutrients, hormones and growth factors which stimulate cell growth. Other serum components serve as binding proteins which promote cell adhesion in vitro.

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  • Mesenchymal stem cells promote endothelial progenitor

    2017-6-9u2002·u2002Bone marrow mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) interact with each other. EPCs are able to promote the self‑renewal of MSCs as niche cells in murine bone marrow, and MSCs are able to promote EPC proliferation in vitro in a co‑culture system. It has previously been reported that MSCs can secrete insulin‑like growth factor‑1 (IGF‑1), which serves ...

    Get Price
  • Human KGF ELISA Kit (ab183362)

    Human Keratinocyte Growth Factor (KGF) is a 28 kDa glycoprotein and the seventh discovered member of the FGF family (FGF-7). It is primarily secreted by cells of mesenchymal origin and it acts in a paracrine fashion on cells expressing the tyrosine kinase receptor FGFR2b.

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  • BJNANO - Mammalian cell growth on gold nanoparticle ...

    2014-12-24u2002·u2002System. We investigate the growth of mammalian epithelial kidney (MDCK II) cells seeded on glass substrates decorated with gold nanorods (38 × 17 nm, Supporting Information of ) and compare the results to cell growth on bare substrates.The study utilized three different surface coatings because the particle-bound molecules (stabilizing agents) are expected to promote diverse interactions with ...

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  • Invitro effect of direct current electrical stimulation on ...

    2017-1-12u2002·u20022:5 104 cell/cm2 until 80% confluency and then expanded over five passages. Cells from passage 6 were seeded in 6-well cell culture plates (TPP, Trasadingen, Switzerland) at a density of 104 cell/cm2 in cell growth medium consisting of Dulbecco's Modified Eagle Medium (DMEM) CGlutaMAX C1 g/L D-Glucose C10% Fetal Calf Serum (FCS) and

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  • Cell Communication in a Coculture System Consisting of ...

    2014-4-22u2002·u2002It is thus possible to promote the metabolic activities of these two cell types within a coculture system. 100,000 primary osteoblasts were seeded at the lower surface and 66,000 OECs were seeded at the upper surface of a polycarbonate transmembrane filter in a Transwell filter system in a 24-well plate (pore size 0.4 μm; 0.588 cm 2 /filter ...

    Get Price
  • Tumor-associated fibroblasts promote the proliferation

    2016-4-11u2002·u2002The reasons for the distinct chemoresistance of liposarcomas and their high risk of local recurrence still remain unclear. Depending on the histological subtype of liposarcoma, first-line therapy with the cytostatic agent, doxorubicin, only achieves response rates of approximately 36%. Approximatley 70% of all local recurrences develop in spite of complete surgical resection of the primary ...

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  • Cell Fate Determination Factor DACH1 Inhibits c-Jun ...

    2006-12-20u2002·u2002Rat1a-J4 cells (4.0 × 10 3) were plated in triplicate in 3 ml of 0.3% agarose (sea plaque) in complete growth medium in the presence or absence of 2 μg/ml doxycycline overlaid on a 0.5% agarose base, also in complete growth medium. Two weeks after incubation, colonies >50 μm in diameter were counted using an Omnicon 3600 image analysis system.

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  • Membrane potential (Vmem) measurements during ... - PeerJ

    2021-10-30u2002·u2002Background Electrochemical signals play an important role in cell communication and behavior. Electrically charged ions transported across cell membranes maintain an electrochemical imbalance that gives rise to bioelectric signaling, called membrane potential or Vmem. Vmem plays a key role in numerous inter- and intracellular functions that regulate cell behaviors like proliferation ...

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  • Cell Culture Serum Products

    Serum is added to what's called basal media to promote growth and maintenance of eukaryotic cells, including mammalian and insect cell lines. This common cell culture supplement provides nutrients to cells. Among these are hormones, growth factors, and minerals. Serum is typically added to basal media to a final concentration of 5% of 10%.

    Get Price
  • Animal‐cell culture media: History, characteristics, and ...

    2017-3-21u2002·u2002Other kinds of serum are used in certain situations, including calf serum (CS) and horse serum. Researchers can select an appropriate serum type on the basis of its characteristics. Fetal bovine serum generally is rich in growth factors and contains low levels of γ‐globulins (which have a cell growth‐inhibitory activity).

    Get Price
  • Nano‑hydroxyapatite/collagen film as a favorable

    the intention to preserve cell phenotype and promote cell growth to ensure sufficient and functional cells, this study may provide novel insights for cell-based therapy. Materials and methods Materials and chemicals. colia 1 of calf origin and nano -Ha 2 powder were purchased from the ngineering esearch e r

    Get Price
  • Biomaterials that promote cell-cell interactions enhance ...

    2017-9-1u2002·u2002MSCs isolated from the tibiae of Sprague Dawley rats via bone marrow biopsies were used in this study. Cell culture was carried out in full culture media i.e. low glucose DMEM containing fetal calf serum (10%), penicillin/streptomycin (1%), and Glutamax™ (1%). Cells were passaged at approximately 70% confluency, and were not used beyond ...

    Get Price
  • Research Article Artificial Extracellular Matrices with ...

    2019-7-31u2002·u2002via LGC Standards GmbH, Wesel, Germany) and cultured according to supplier s recommendations. MLO-Y cell line was described in detail by Kato et al. [ ] and was a kind gi from Lynda Bonewald (Kansas City, USA). Cell characteristics, plating density, and media used for cell expansion are given in Table . In order to have the di erent cell (line ...

    Get Price
  • Allergan: A Bold, Global Pharmaceutical Company

    Allergan plc (NYSE: AGN), headquartered in Dublin, Ireland, is a global pharmaceutical leader focused on developing, manufacturing and commercializing branded pharmaceutical, device, biologic, surgical and regenerative medicine products for patients around the world.

    Get Price
  • Research Article Endothelial Progenitor Cell Fraction ...

    2019-7-31u2002·u2002medium containing autologous growth factors (PL) that was showntopromoteMSCsdi erentiation[,]. 2. Materials and Methods.. Cell Culture Media. Iscove s Modi ed Dulbecco s Medium (IMDM), Fetal Calf Serum (FSC), Nonessential Amino Acids (NEAA), and antibiotics (PenStrep, PS) were purchased from Gibco/Invitrogen Life Technologies (Zug, Switzerland).

    Get Price
  • Cell Communication in a Coculture System Consisting of ...

    2014-4-22u2002·u2002It is thus possible to promote the metabolic activities of these two cell types within a coculture system. 100,000 primary osteoblasts were seeded at the lower surface and 66,000 OECs were seeded at the upper surface of a polycarbonate transmembrane filter in a Transwell filter system in a 24-well plate (pore size 0.4 μm; 0.588 cm 2 /filter ...

    Get Price
  • Tumor-associated macrophages promote ovarian cancer

    2020-4-20u2002·u2002Steitz, A.M., Steffes, A., Finkernagel, F. et al. Tumor-associated macrophages promote ovarian cancer cell migration by secreting transforming growth factor beta induced (TGFBI) and …

    Get Price
  • Sildenafil Potentiates a cGMP-Dependent Pathway to

    Use of the phosphodiesterase 5 inhibitor sildenafil (Viagra) has been linked to an increased risk of melanoma. Dhayade et al. explore the underlying mechanism and identify a growth-promoting cGMP-MAPK pathway in melanoma cells that is potentiated by sildenafil treatment.

    Get Price
  • Cell Culture Serum Products

    Serum is added to what's called basal media to promote growth and maintenance of eukaryotic cells, including mammalian and insect cell lines. This common cell culture supplement provides nutrients to cells. Among these are hormones, growth factors, and minerals. Serum is typically added to basal media to a final concentration of 5% of 10%.

    Get Price
  • Animal‐cell culture media: History, characteristics, and ...

    2017-3-21u2002·u2002Other kinds of serum are used in certain situations, including calf serum (CS) and horse serum. Researchers can select an appropriate serum type on the basis of its characteristics. Fetal bovine serum generally is rich in growth factors and contains low levels of γ‐globulins (which have a cell growth‐inhibitory activity).

    Get Price
  • Nano‑hydroxyapatite/collagen film as a favorable

    the intention to preserve cell phenotype and promote cell growth to ensure sufficient and functional cells, this study may provide novel insights for cell-based therapy. Materials and methods Materials and chemicals. colia 1 of calf origin and nano -Ha 2 powder were purchased from the ngineering esearch e r

    Get Price
  • Biomaterials that promote cell-cell interactions enhance ...

    2017-9-1u2002·u2002MSCs isolated from the tibiae of Sprague Dawley rats via bone marrow biopsies were used in this study. Cell culture was carried out in full culture media i.e. low glucose DMEM containing fetal calf serum (10%), penicillin/streptomycin (1%), and Glutamax™ (1%). Cells were passaged at approximately 70% confluency, and were not used beyond ...

    Get Price
  • Research Article Artificial Extracellular Matrices with ...

    2019-7-31u2002·u2002via LGC Standards GmbH, Wesel, Germany) and cultured according to supplier s recommendations. MLO-Y cell line was described in detail by Kato et al. [ ] and was a kind gi from Lynda Bonewald (Kansas City, USA). Cell characteristics, plating density, and media used for cell expansion are given in Table . In order to have the di erent cell (line ...

    Get Price
  • Allergan: A Bold, Global Pharmaceutical Company

    Allergan plc (NYSE: AGN), headquartered in Dublin, Ireland, is a global pharmaceutical leader focused on developing, manufacturing and commercializing branded pharmaceutical, device, biologic, surgical and regenerative medicine products for patients around the world.

    Get Price
  • Research Article Endothelial Progenitor Cell Fraction ...

    2019-7-31u2002·u2002medium containing autologous growth factors (PL) that was showntopromoteMSCsdi erentiation[,]. 2. Materials and Methods.. Cell Culture Media. Iscove s Modi ed Dulbecco s Medium (IMDM), Fetal Calf Serum (FSC), Nonessential Amino Acids (NEAA), and antibiotics (PenStrep, PS) were purchased from Gibco/Invitrogen Life Technologies (Zug, Switzerland).

    Get Price
  • Cell Communication in a Coculture System Consisting of ...

    2014-4-22u2002·u2002It is thus possible to promote the metabolic activities of these two cell types within a coculture system. 100,000 primary osteoblasts were seeded at the lower surface and 66,000 OECs were seeded at the upper surface of a polycarbonate transmembrane filter in a Transwell filter system in a 24-well plate (pore size 0.4 μm; 0.588 cm 2 /filter ...

    Get Price
  • Tumor-associated macrophages promote ovarian cancer

    2020-4-20u2002·u2002Steitz, A.M., Steffes, A., Finkernagel, F. et al. Tumor-associated macrophages promote ovarian cancer cell migration by secreting transforming growth factor beta induced (TGFBI) and …

    Get Price
  • Sildenafil Potentiates a cGMP-Dependent Pathway to

    Use of the phosphodiesterase 5 inhibitor sildenafil (Viagra) has been linked to an increased risk of melanoma. Dhayade et al. explore the underlying mechanism and identify a growth-promoting cGMP-MAPK pathway in melanoma cells that is potentiated by sildenafil treatment.

    Get Price