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  • Serum-free medium and hydroxyethyl starch supports cell ...

    Background/aims: In a previous study, we observed a deleterious effect of serum-supplemented Minimal Essential Medium (MEM) on human corneal endothelial cell survival in a cell culture model. Consequently, here we studied the effects of conventional, serum-supplemented MEM and a serum-free medium in combination with two different deswelling substances on cell survival in whole corneas in a ...

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    2018-10-15u2002·u2002with 10% heat-inactivated (56°C, 30 min) bovine calf serum (CFS). Cells were maintained at 37°C in a humidified 5% CO 2 atmosphere throughout the experiments. For subculture, the medium was removed, and cells were detached with 0.25% trypsin in a Ca2+-, Mg2+-free phosphate-buffered saline (PBS) containing 0.2 g/L EDTA.

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    2021-5-31u2002·u2002The 'Global Bovine Newborn Calf Serum Market Size, Global Trends, Growth, Opportunities, Market Share and Market Forecast – 2021 – 2028' is a comprehensive and detailed report published by Zeal Insider'.The report provides all the latest details regarding impact of covid-19 on the market and its comparison with pre covid-19 situation. Therefore, the report aims to cover statistics of ...

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    Next, fetal calf serum and bovine serum albumin present in the standard medium were replaced by a custom-made and xeno-free B-27. This yielded in a chemically defined and xenogeneic-free 3D culture protocol for differentiation of hPSCs into DE at efficiencies similar to standard 2D conditions.

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    2001-6-1u2002·u2002Caco-2 cells were grown either serum free or with 1% FCS and then cultured in the presence of 5–500 μmol/L tributyrin for 12 h. Total RNA was isolated by RNeasy Mini Kit (Qiagen, Hilden, Germany) according to manufacturer's instructions and quantitated by measuring the absorbance at 260 nm. RT-PCR was performed as described (22).

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    2021-9-9u2002·u2002DMEM high glucose (Gibco, Darmstadt, Germany) containing 10% fetal calf serum (FCS) (PAA, Freiburg, Germany), 1% penicillin streptomycin (Gibco). The medium was changed twice weekly. Microglia cells were harvested by shaking for 1 h at 200 rpm and re-seeded in 24-well plates for stimulation experiments at a density of 1.5 510 cells/ml.

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    2012-1-14u2002·u2002supplemented with 10% fetal calf serum (Hyclone, Logan, UT, USA) and plated (50 μl/well) in 96-well cell culture plate (Corning, Corning, NY, USA) overnight at 37˚C with 5% CO. 2. and 95% humidity. Fifty microliters of serial 2-fold diluted sterile MAb1E4-1C2 or MAb anti-HIV p24 (isotype control) were added to final

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    2019-11-13u2002·u2002AUTOIMMUNE, CHOLESTATIC AND BILIARY DISEASE a 5b 1-Integrins Are Sensors for Tauroursodeoxycholic Acid in Hepatocytes Holger Gohlke,1 Birte Schmitz,1 Annika Sommerfeld,2 Roland Reinehr,2 and Dieter H€aussinger 2 Ursodeoxycholic acid, which in vivo is converted to its taurine conjugate tauroursodeoxy-

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  • Serum-free medium and hydroxyethyl starch supports cell ...

    Background/aims: In a previous study, we observed a deleterious effect of serum-supplemented Minimal Essential Medium (MEM) on human corneal endothelial cell survival in a cell culture model. Consequently, here we studied the effects of conventional, serum-supplemented MEM and a serum-free medium in combination with two different deswelling substances on cell survival in whole corneas in a ...

    Get Price
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  • Potential of Coffee Fruit Extract and Quinic Acid on ...

    2018-10-15u2002·u2002with 10% heat-inactivated (56°C, 30 min) bovine calf serum (CFS). Cells were maintained at 37°C in a humidified 5% CO 2 atmosphere throughout the experiments. For subculture, the medium was removed, and cells were detached with 0.25% trypsin in a Ca2+-, Mg2+-free phosphate-buffered saline (PBS) containing 0.2 g/L EDTA.

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    Albania Algérie Andorra Armenia Argentina Aruba Australia Azerbaijan Bahrain Belgium Беларусь/Belarus Bosnia And Herzegovina Brasil България Bulgaria Canada Chile MAINLAND CHINA 中国大陆 Hong Kong SAR 香港特別行政區 Macau SAR 澳門特別行政區 Taiwan, China 中國台灣 Colombia Costa Rica Cyprus Česká republika Danmark Deutschland Germany Ecuador ...

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    Discover the official dunhill online store. Browse our collection of men's ready-to-wear, suits, shoes, leather goods and accessories.

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    lululemon makes technical athletic clothes for yoga, running, working out, and most other sweaty pursuits. As always, shipping is free.

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